γ-氨基丁酸代谢旁路阻断对苏云金芽胞杆菌芽胞形成和晶体蛋白产量的影响

γ-氨基丁酸旁路(GABA shunt)普遍存在于原核和真核生物体中,对于动物、植物和微生物具有不同的生理功能.苏云金芽胞杆菌gab基因簇中gabT和gabD基因所编码的γ-氨基丁酸转氨酶和琥珀酸半醛脱氢酶参与GABA shunt.在前期研究获得的苏云金芽胞杆菌HD-73菌株gabT、gabR(gab基因簇中的调控基因)和gabD基因缺失突变菌株的基础上,从菌株生长、芽胞产量和晶体蛋白产量等方面研究GABA shunt阻断对Bt芽胞和晶体形成的影响.结果表明,GABA shunt阻断对芽胞产量有一定影响,但对Bt生长和晶体蛋白产量无明显影响     

Imaging diagnosis--Ventriculo-peritoneal shunt associated infection in a dog

Ventriculo-peritoneal shunting is a surgical treatment for hydrocephalus. Complications of this procedure are not well described in dogs. The most common complication in humans is infection, which can be fatal if not diagnosed and treated quickly. We describe the magnetic resonance (MR) imaging characteristics of a shunt-associated cerebral infection in a dog. The MR features of the infection included hyperintensity of the lining of the ventricular system visible on a T2-weighted FLAIR sequence and marked linear contrast enhancement of the ependymal layer on T1-weighted sequences, similar to that described in people.     

自动卫生筷子机成品分流器的改进

针对日本进口的卫生筷子自动生产机床出料端没有成品筷子大小头分流器而只能人工分选这一问题，在机床后端一侧设计了一套齿轮传动的偏心轮摇杆机构，实现了成品筷子大小头的自动分选。     

Comparison of single-breath continuous positive airway pressure manoeuvre with inhaled salbutamol to improve oxygenation in horses anaesthetized for laparotomy

ObjectiveTo compare the efficacy of single-breath continuous positive airway pressure manoeuvre (CPAP-M) with inhaled salbutamol, and a combination of both.Study designRandomized, clinical study.AnimalsA total of 62 client-owned horses (American Society of Anesthesiologists status III–V) anaesthetized for laparotomy.MethodsHorses were premedicated with intravenous (IV) xylazine (0.4–0.6 mg kg^–1), anaesthesia was induced with midazolam (0.06 mg kg^–1 IV) and ketamine (2.2 mg kg^–1 IV) and maintained with isoflurane in oxygen using volume-controlled ventilation without positive end-expiratory pressure. If PaO₂ was < 100 mmHg (13.3 kPa), either a CPAP-M (50 cmH₂O for 45 seconds) or salbutamol (0.002 mg kg^–1) was administered. The intervention was considered successful if PaO₂ reached 100 mmHg (13.3 kPa). If PaO₂ remained < 100 mmHg (13.3 kPa), treatments were switched. PaO₂/FiO₂ ratio and estimated shunt fraction (F-shunt) were derived from data obtained from arterial blood gas measurements. Dynamic compliance (C_dyn) was calculated from variables recorded at the moment of arterial blood analysis. Fisher’s exact tests compared success rates between treatments, and linear models were performed to test whether the treatment modified the values of the measurements; p < 0.05.ResultsSalbutamol was the first intervention in 28 horses and was effective in 22 horses. CPAP-M was the first intervention in 34 horses and was effective in 26 horses. CPAP-M after salbutamol was performed in six horses, with four responders, and salbutamol after CPAP-M was administered to eight horses, with one responder. Salbutamol, but not CPAP-M, significantly decreased F-shunt. Both salbutamol and CPAP-M significantly increased C_dyn.Conclusions and clinical relevanceSalbutamol and CPAP-M were comparably effective in improving oxygenation and C_dyn in anaesthetized horses with PaO₂ < 100 mmHg (13.3 kPa). Whether combining both treatments might be beneficial needs to be confirmed on a larger number of horses.     

IMAGING DIAGNOSIS—PORTAL VEIN APLASIA AND INTERRUPTION OF THE CAUDAL VENA CAVA IN THREE DOGS

Severe portal vascular anomalies have been reported previously accompanying azygos continuation of the caudal vena cava, polysplenia, and situs anomalies in dogs and people. Three dogs with portal vascular anomalies were identified by means of CT angiography as having portal vein aplasia with portal insertion into the caudal vena cava, azygos continuation of the caudal vena cava, and interruption of the pre‐hepatic caudal vena cava. This information confirms that complex embryological defects may occur in patients presenting for congenital portosystemic shunt, and that CT angiography is a non‐invasive method of completely evaluating these potentially non‐surgical portal vascular anomalies.     

菜籽油甲酯化及其动力学研究

本文报道菜籽油甲酯化反应的适宜条件为菜籽油/甲醇（摩尔比）为1/6,0.5％NaOCH_3作催化剂,反应温度40～60℃之间,反应时间在1小时内。用NMR—24B型核磁共振仪作为分析检测手段。动力学研究表明,该反应分为两个阶段:开始的四级对峙反应,速度常数的数量级为10~（-4）、表观活化能65.2KJ·mol~（-1）;后面的二级对峙反应,速度常数的数量级为10~（-2）。本研究为菜籽油甲酯化在工业上生产加脂剂提供了理论上的参考数据。