不同bFF对延边黄牛卵母细胞成熟及发育的影响

在成熟液中添加不同处理及来源的牛卵泡液(bFF),以研究其对延边黄牛卵母细胞体外成熟及重组胚发育的影响。结果表明:未抽滤组与抽滤组间成熟率差异不显著(P>0.05),但未抽滤组显著提高囊胚率(P<0.05);卵泡直径2~8 mm组、>8 mm组和混合组(2~8 mm组与>8 mm组的混合)之间成熟率、卵裂率和囊胚发育率差异不显著(P>0.05);两试验组(黄体组、卵泡组)成熟率、卵裂率和囊胚率差异不显著(P>0.05),两试验组与血清组相比囊胚率显著提高(P<0.05)。试验结果提示,未抽滤黄体期卵巢表面卵泡内bFF能有效提高卵母细胞体外囊胚发育率。     

不同日粮组合对杂交肉牛生长性能的影响

对肉牛饲料进行营养价值评定,并选择55头西门达尔杂交肉牛分5组进行试验,研究不同日粮组合对杂交肉牛生产性能的影响。结果显示,肉牛代谢体重干物质采食量与日粮粗蛋白含量之间存在极显著正相关关系,日增重随代谢体重采食量的增加呈直线增加,经济效益随肉牛日增重的增加呈直线增加。以秸秆 野草 0.7 kg玉米面、秸秆 野草 0.5 kg肉牛浓缩料、秸秆 牧草 0.5 kg肉牛浓缩料、秸秆 野草 2 kg肉牛精料补充料和秸秆 牧草 2 kg肉牛精料补充料5种日粮组合分别饲喂西杂肉牛63~75 d,日增重分别为0.14,0.32,0.63,0.90和1.08 kg,体况评分分别增加0.10,0.17,0.33,0.48和0.77分,日盈利分别为-0.36,0.76,3.07,5.74和6.13元。结果表明,在传统饲养模式下,改善粗饲料品质、补充含蛋白质和矿物质的精料可显著提高肉牛生产性能和经济效益。     

奶牛皱胃变位的诊治

近年来,随着我国奶牛业的迅速发展和牛奶产量的不断提高,皱胃变位作为一种生产性疾病其发病数量也随之增多。奶牛皱胃变位多发生于产后不久的高产奶牛,直接影响了奶牛的生产性能,对畜牧业生产造成的损失较大。笔者就奶牛皱胃变位的病因、诊断及治疗进行了探讨。     

湖北省短轮伐期用材林基地建设探讨

笔者简要分析了短周期工业原料林建设的现状,概述了其基地建设的布局原则,并按其原则对湖北省适合短轮伐期用材林发展区域进行了重点布局;针对性地提出了相应的技术措施,为短周期原料林基地建设提供参考。     

The expected utility of genetic information in beef cattle production

Scientific inquiry is increasing our knowledge of plant and animal genomics. The ability to specify heterogeneous production processes, to sort agricultural inputs by genotype, or to guide breeding programs to satisfy specific markets based on genetic expression may increase producer and consumer benefits. This research develops a decision analysis framework to assess the expected value of genetic information under risk aversion. Expected utilities are evaluated both with and without genetic trait information. Potential gains in the value of information can be quantified as research unravels the linkages between genetics and crop and animal performance and quality. An application to cattle feeding indicates potential gains to developing markets for specific animal genetic characteristics based on the amino acid sequence of the leptin gene.     

10kV、630kvA及以下配电变压器保护配置方式的合理选择

10kV配电变压器的保护配置主要有断路器、负荷开关或负荷开关加熔断器等。对10kV环网供电单元和终端用户10 kV配电变压器采用断路器、负荷开关加熔断器组合的保护配置方式进行技术、经济比较,供配电网的设计和运行管理部门参考。     

衣滞康散对胎衣不下奶牛血液流变学指标的影响

1材料与方法 1．1药物衣滞康散主要成分为血竭、桃仁、红花、当归、木鳖子、土鳖虫、益母草等,由石河子大学科技处研制并保存。     

The Structure and Sequence Analysis of TLR4 Gene in Cattle

Toll-like receptor 4 （TLR4） is essential for initiating the innate response to lipopolysaccharide （LPS） from Gram-negative bacteria by acting as a signal transducting receptor. In order to help in investigating TLR4 as a candidate disease-resistance gene in cows, we isolated the cDNA （GenBank accession no. DQ839566） by RT-PCR and rapid amplification of cDNA ends （RACE） experiments and analyzed the sequence characters by bioinformatics. The results showed that cattle TLR4 gene about 3 739 bp contains an open reading frame of 2 526 bp encoded 841 amino acids （aa）, 470 bp 5′ untranslated region （UTR）, and 743 bp 3′ UTR. Tissue expression profile by RT-PCR indicated that TLR4 gene expresses in mammary glands, liver, muscle, duodenum, fats, uterus, kidneys, hearts, lungs, pancreas, and ovary. TLR4 protein domain predicted by bioinformatics consists of signal peptide, transmembrane helices domain, 3 sorts of leucine-rich repeat domains （LRR, LRR-TYP, and LRRCT）, and a toll-interleukinl-resistance domain （TIR）. Leucine-rich repeat domains were related with recognizing a broad of pathogen-associated molecular patterns （PAMP） from pathogen, and TIR domain for downstream signaling transduction was most conservative （98% identify） than other domains after alignment of protein from ovine, porcine, human, and mouse. In addition, a 470 bp 5′-flanking region sequence was amplified by PCR, and 15 putative DNA binding sites were predicted, but this sequence lacks TATA box, CCAAT character, and GC-rich regions.     

日粮粗蛋白质及能量水平对架子牛肥育效果影响的研究

试验采用2～3岁,体重在305～350kg的架子牛,按高蛋白高能量(精料1号)、高蛋白低能量(精料2号)、低蛋白高能量(精料3号)、低蛋白低能量(精料4号)四组日粮进行为期90d的肥育。试验结果表明,当架子牛体重在320～420kg的情况下,高蛋白高能量日粮组日增重明显高于其他三组(P<0.01)。当体重超过420kg以上时,高蛋白高能量组日增重与低蛋白高能量组比较差异显著(P<0.05),与其他两组比较差异极显著(P<0.01)。从经济效益上分析,由高到低,依次为高蛋白高能量、低蛋白高能量、高蛋白低能量、低蛋白低能量。结论:架子牛肥育前期日粮(按风干计)的粗蛋白质水平应达到13.5%～14.0%,消化能在11.60～11.70MJ/kg;肥育后期粗蛋白质水平应达到12%,消化能要在11.80MJ/kg以上。     

我国肉牛的竞争力分析与财政支持政策

本文介绍了我国肉牛生产现状、国际和国内牛肉市场与我国肉牛出口和进口情况,分析了制约我国肉牛竞争力的因素,提出了在国际市场增加肉牛竞争力的财政支持政策。