全文获取类型
收费全文 | 486篇 |
免费 | 48篇 |
国内免费 | 38篇 |
专业分类
林业 | 14篇 |
农学 | 26篇 |
基础科学 | 7篇 |
38篇 | |
综合类 | 135篇 |
农作物 | 9篇 |
水产渔业 | 15篇 |
畜牧兽医 | 325篇 |
植物保护 | 3篇 |
出版年
2024年 | 3篇 |
2023年 | 4篇 |
2022年 | 18篇 |
2021年 | 31篇 |
2020年 | 14篇 |
2019年 | 31篇 |
2018年 | 21篇 |
2017年 | 43篇 |
2016年 | 24篇 |
2015年 | 26篇 |
2014年 | 32篇 |
2013年 | 31篇 |
2012年 | 41篇 |
2011年 | 41篇 |
2010年 | 28篇 |
2009年 | 22篇 |
2008年 | 43篇 |
2007年 | 21篇 |
2006年 | 22篇 |
2005年 | 9篇 |
2004年 | 11篇 |
2003年 | 11篇 |
2002年 | 15篇 |
2001年 | 3篇 |
2000年 | 5篇 |
1999年 | 3篇 |
1998年 | 3篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 5篇 |
1991年 | 2篇 |
1990年 | 3篇 |
1988年 | 1篇 |
排序方式: 共有572条查询结果,搜索用时 0 毫秒
111.
【目的】探讨屎肠球菌对瘤胃发酵的影响,以此评价屎肠球菌能否作为直接饲用微生物用于反刍动物的生产。【方法】利用持续动态人工瘤胃装置,采用完全随机试验设计,以不添加屎肠球菌发酵液为对照,研究了添加1.25×106和6.25×106mL-1屎肠球菌发酵液对瘤胃液pH、微生物蛋白(MCP)、氨态氮(NH3-N)、乙酸、丙酸、丁酸和总挥发性脂肪酸(TVFA)浓度的影响。【结果】添加屎肠球菌对瘤胃pH值和MCP无显著影响(P>0.05),但能显著提高瘤胃NH3-N浓度(P<0.01);与对照组相比,1.25×106mL-1屎肠球菌液添加组显著提高了瘤胃液的乙酸与总挥发性脂肪酸浓度(P<0.05),而添加屎肠球菌对瘤胃丙酸和丁酸浓度及乙酸与丙酸的比例没有显著影响(P>0.05)。【结论】添加一定量的屎肠球菌,可在不改变瘤胃发酵类型的情况下促进饲料中碳水化合物的消化。 相似文献
112.
113.
本研究旨在探究饲粮蛋白质水平对藏系绵羊瘤胃真菌菌群结构及功能的影响。试验选取18只12月龄健康、平均体重为(31.71±0.72)kg的藏系绵羊羯羊,随机分为3组,每组6只,分别饲喂代谢能相近而蛋白质含量不同(LP组,10.06%CP;MP组,12.10%CP;HP组,14.12%CP)的饲粮,试验为期120 d,包括15 d的预饲期和105 d的正试期。结果表明:1)LP组藏系绵羊的终末体重、平均日增重和平均日采食量均显著低于MP组和HP组(P<0.05),而料重比显著高于MP组和HP组(P<0.05)。2)3组18个瘤胃液样品共产生1547415条有效序列,聚类后共得到4073个OTUs。饲粮蛋白质水平并没有对藏系绵羊瘤胃真菌多样性指数产生显著性影响(P>0.05)。3)在门分类水平上,藏系绵羊瘤胃真菌优势菌门为子囊菌门、担子菌门、被孢霉亚门和新美鞭菌门等;在属分类水平上,瘤胃真菌优势菌属为青霉属、无茎真菌属、枝孢属、镰孢霉属和链格孢属等。4)采用LEfSe方法对各个分类水平上丰度有显著差异的微生物进行比较分析,共筛选到33个符合生物标记物的真菌菌群。5)基于FUNGuid对藏系绵羊瘤胃真菌群落的营养型进行功能预测,发现腐生营养型是最主要的营养型。以上结果表明,适当提高饲粮中蛋白质水平可以显著提高藏系绵羊的生长性能,但对瘤胃真菌菌群的多样性和结构组成并未产生明显的影响。 相似文献
114.
This research proposes a batch in vitro rumen apparatus modified from Daisy incubator and procedures of measuring feed digestion by using nylon bag to replace the traditional centrifuging and filtration methods, for the purpose of studying ruminal fermentation. The apparatus consists of 8 sets of airtight reaction vessels which rotated at a speed of 1 r/min and located inside a thermostatic cabin. Optimum procedures was proved to be that the volume of incubation fluid was 400 mL; fineness and size of test feed were 0.125-0.25 mm and 0.4 g, respectively; amount of bags in one vessel was 6; fermentation diet in a single incubation was 4-5 g. Comparing experiments showed that fermentation speed attained with the apparatus was about 15-20% greater than that attained with flask-shaking machine method (revolutionary speed is 100 r/min) and more accurate results could be obtained with the new apparatus. In conclusion, this apparatus could provide an efficient mixing action, and the procedure could allow sensitive detection of differences in ruminal fermentation in a time-saving way. 相似文献
115.
Herbage intake and ruminal digestion of dairy cows grazed on perennial ryegrass pasture either in the morning or evening 下载免费PDF全文
This study aimed to clarify diurnal fluctuations of herbage intake, ruminal fermentation of herbage carbohydrates and proteins, and digesta particulate weight in the rumen of grazing dairy cows. Six ruminally cannulated, non‐lactating dairy cows were grazed on perennial ryegrass/white clover pasture either in the morning (04.00 to 08.00 hours) or the evening (16.00 to 20.00 hours). Cows grazed in the evening spent more time (P < 0.01) and consumed more herbage (P < 0.01) compared with cows grazed in the morning. Higher (P < 0.05) daily mean concentrations of total volatile fatty acid, propionate and n‐butyrate in rumen fluid were observed for cows grazed in the evening compared with cows grazed in the morning. Although cows grazed in the evening ingested more crude protein compared with cows grazed in the morning, no significant difference in NH3‐N concentration in rumen fluid was observed between them. The ratio of purine‐derivative concentration to creatinine concentrations was higher (P < 0.01) in the urine of cows grazed in the evening than in cows grazed in the morning. These results clearly indicated that evening grazing was advantageous for dairy cows compared with morning grazing, in terms of ruminal fermentable energy intake and nitrogen utilization efficiency. 相似文献
116.
Influence of harvesting site on chemical composition and potential protein value of Acacia erioloba,A. nilotica and Ziziphus mucronata leaves for ruminants 下载免费PDF全文
This study was designed to evaluate the chemical composition, buffer N solubility, in vitro ruminal N degradation and in vitro ruminal biological activity of condensed tannins in Acacia erioloba, Acacia nilotica and Ziziphus mucronata leaves harvested from two sites (Masuthle communal grazing land and Molelwane private farm). Leaves were harvested, dried at 60 °C and milled. The highest crude protein (CP) content was found in leaves of Z. mucronata (177.7 g/kg DM). Leaves harvested from Masuthle had higher (p < 0.05) soluble phenolics (SPh) (44.6 g TAE/kg DM) compared to those harvested from Molelwane (29.8 g TAE/kg DM). In both Molelwane and Masuthle, leaves of A. nilotica had higher levels of condensed tannins (CT) (0.76 AU550/200 mg and 0.52 AU550/200 mg respectively) followed by A. erioloba and Z. mucronata, which did not differ (p > 0.05). Nitrogen degradability at 24 h was the same (p > 0.05) for all tree species but not at 12 and 36 h. No linear association (p > 0.05) was found between buffer‐soluble N and in vitro ruminal N degradability in leaves with high SPh content. The largest polyethylene glycol (PEG) effect was in leaves of A. nilotica (448%) harvested from Masuthle after 36 h of incubation. Ziziphus mucronata leaves harvested from Molelwane had the least PEG effect at 48 h. There was no linear association (p > 0.05) between PEG effect and SPh for all incubation periods, but a positive relationship was observed between PEG effect and condensed tannins content. There was no linear association between solubility index (SI) and in vitro ruminal N degradability for tannin‐rich leaves. It was concluded that higher browsing pressure in Masuthle communal rangeland resulted in leaves with higher levels of condensed tannins, which had higher in vitro ruminal biological activity, compared to those harvested from Molelwane. 相似文献
117.
微生物学实验是一门非常重要的基础课实验,在微生物学的教学中占有举足轻重的地位。通过合理地设置实验内容,采用科学的教学方法,提高了教学效果,培养了学生观察、思考、分析和解决问题的综合能力,为学生进一步学习有关专业课程打下良好基础。 相似文献
118.
Effects of different forage combinations in total mixed rations on in vitro gas production kinetics,ruminal and milk fatty acid profiles of lactating cows 下载免费PDF全文
Sarvvl Bai Zhi‐Jun Cao Bin‐Bin Cao Hong‐Jian Yang Sheng‐Li Li Jian‐Xin Liu 《Animal Science Journal》2018,89(9):1261-1270
This study aimed to determine the effects of different forage combinations on in vitro gas production (GP) kinetics, ruminal and milk fatty acid profiles. Forty‐five lactating cows were randomly arranged into three groups and fed three total mixed rations (TMRs) with different forage combinations: TMR1, 23% alfalfa hay, 7% Chinese wild ryegrass hay and 15% whole corn silage; TMR2, 30% corn stover plus 15% whole corn silage; TMR3, 30% rice straw plus 15% whole corn silage. In vitro dry matter disappearance ranked: TMR1 > TMR2 > TMR3, and highest cumulative GP and asymptotic GP occurred in TMR1 while no difference occurred between TMR2 and TMR3. The average GP rate ranked: TMR1 > TMR2 > TMR3. TMR1 in comparison with TMR2 and TMR3 presented lower rumen contents of acetate and butyrate and greater rumen contents of propionate, valerate, C13:0, C14:0, C15:0, C18:1cis‐9, C18:2n‐6, C18:3n‐3, C20:0 and C22:0 as well as milk C18:2n‐6 and C18:3n‐3 proportions. Transfer efficiencies of C18:2n‐6 and C18:3n‐3 from diet to milk ranked: TMR1 > TMR2 > TMR3. The findings suggest TMRs containing alfalfa hay and Chinese wild ryegrass hay in comparison with corn stover or rice straw improve rumen fermentation and transfer efficiency of C18:2n‐6 and C18:3n‐3. 相似文献
119.
本试验研究了日粮中不同中性洗涤纤维/非纤维性碳水化合物(NDF/NFC)水平对周岁后荷斯坦奶牛生产性能、营养物质消化率、瘤胃发酵特征及甲烷产量的影响,并在此基础上建立了甲烷排放预测模型,旨在获得我国生产模型下的甲烷排放规律和甲烷转化因子,为提高奶牛能量利用效率、建立国家或区域性温室气体排放清单和探索减排策略提供科学依据和支撑。将45头体况良好,平均为15月龄的荷斯坦后备奶牛随机分为3组,每组15头牛:低日粮NDF/NFC组(NDF/NFC=0.60)、中日粮NDF/NFC组(NDF/NFC=0.75)和高日粮NDF/NFC组(NDF/NFC=0.90),试验期为70 d,包括14 d的预饲期和56 d的正试期。结果表明:1)提高日粮NDF/NFC水平显著降低了奶牛的干物质采食量、有机物采食量、平均日增重、干物质和粗蛋白的表观消化率(P<0.05);2)提高日粮NDF/NFC水平显著增加了瘤胃内总挥发性脂肪酸产量、乙酸的相对含量和乙酸/丙酸比例(P<0.05),显著降低了丙酸的相对含量(P<0.05);3)随着日粮NDF/NFC水平的提高,瘤胃甲烷和甲烷能产量、甲烷/代谢体重、甲烷/干物质采食量、甲烷/有机物采食量、甲烷/中性洗涤纤维采食量显著提高(P<0.05)。甲烷转化因子也随着日粮NDF/NFC水平的增加而显著提高(P<0.05);4)基于体重、采食量、营养物质含量和NDF/NFC分别建立了甲烷预测模型,其中基于干物质采食量和中性洗涤纤维采食量建立的预测模型的决定系数最高(R2=0.77)。因此,提高日粮中NDF/NFC水平可显著降低周岁后荷斯坦奶牛的生产性能、营养物质消化率和瘤胃内丙酸的相对含量,可显著提高瘤胃甲烷产量和甲烷转化因子。 相似文献
120.
试验通过比较不同断奶日龄的湖羊羔羊瘤胃组织结构的差异,特别是瘤胃乳头超微结构变化,旨在为羔羊适宜断奶日龄的确定提供瘤胃生长发育的依据。选择新生湖羊羔羊24只(初生重:2.80 kg±0.08 kg),随机分为3组,每组8只,分别为30日龄早期断奶组(EEW-30)、45日龄早期断奶组(EW-45)和60日龄常规断奶组(NW-60)。在各断奶日龄及75日龄时每组分别屠宰4只羔羊,测定体重及瘤胃空重,计算瘤胃相对重量;采集瘤胃组织进行显微及超微形态学测定。结果显示:①EEW-30组羔羊断奶体重极显著低于NW-60组(P<0.01),而EW-45组与NW-60组断奶体重无显著差异(P>0.05);75日龄时3组羔羊的体重无显著差异(P>0.05)。各组羔羊瘤胃相对重量与其体重变化规律相同。②光镜下可见:断奶时,EEW-30组瘤胃壁厚度极显著低于NW-60组(P<0.01),EW-45组与NW-60组无显著差异(P>0.05);75日龄时,EW-45组羔羊瘤胃壁厚度显著高于NW-60组(P<0.05)。断奶时EEW-30组瘤胃乳头较短小,极显著短于NW-60组(P<0.01),EW-45组也显著短于NW-60组(P<0.05)。断奶时瘤胃乳头宽度和面积在3组间差异均不显著,在75日龄时也无显著组间差异(P>0.05)。③扫描电镜观测结果显示:EEW-30组断奶时单位面积瘤胃微乳头(ruminal micro-papillae)数量极显著低于NW-60组(P<0.01),而EW-45与NW-60组间无显著差异(P>0.05);75日龄时,3组羔羊单位面积瘤胃微乳头数量无显著差异(P>0.05)。上述试验结果显示,断奶时羔羊瘤胃形态结构在EEW-30与NW-60组间差异明显,而EW-45与NW-60组较为接近,瘤胃微乳头状结构也呈相同变化趋势。提示45日龄时断奶较为适宜。 相似文献