ABT1号生根粉在沙棘嫩枝扦插育苗中的应用

用不同浓度ABT1号生根粉处理沙棘嫩枝后进行扦插,结果表明：ABT1号生根粉可提高沙棘嫩枝的生根率并增加根的数量,各处理下生根率较对照高21%～79%,其中以100×10-6浓度处理1 h为最好,根数是对照的2.3倍～3.0倍;ABT1号生根粉对平均根长和最长根长也有极显著的促进作用。同时发现,插穗选择带顶芽或枝刺的半木质化枝条生根率高。     

生根剂促进榕树和小天使生根的试验

为了提高水培观赏植物小天使和榕树的成活率,用"ABT生根粉"配成六种不同浓度生根剂对植物生根影响进行研究。试验发现:草本植物小天使适合的生根剂浓度为2000mg/kg,木本植物榕树适合的生根剂浓度为5000mg/kg。     

生长调节物质对欧洲甜樱桃快速繁殖的影响

以欧洲甜樱桃试管苗为材料,采用正交设计,研究了几种生长调节物质(6-BAI、BA、NAAI、AA、TDZ)对欧洲甜樱桃快速繁殖的影响。结果表明:继代培养中,6-BA和IBA对提高培养效果的影响均极显著,6-BA可诱导丛生芽的形成,IBA配合6-BA可促进丛生芽和叶的生长,而微量的TDZ即可极显著地抑制培养效果。生根培养中,NAA和IBA可极显著地促进生根培养效果,其中NAA主要诱导根的发生并促进根的伸长,IBA刺激根数的增加并促进根和苗的生长,而IAA对试管苗生根无显著作用。试验筛选出适宜的继代培养基为MS+6-BA 0.8 mg/L+IBA 0.3 mg/L+砂糖30 g/L+琼脂6.0 g/L;适宜的生根培养基为1/2MS+NAA 0.8 mg/L+IBA 0.6 mg/L+蔗糖20 g/L+琼脂6.0 g/L。     

"长白山"欧李全光照弥雾扦插技术研究

在全光照弥雾条件下,以"长白山"欧李为试料,以洁净的河沙、细炉灰、田土为基质,以ABT1号生根粉4种浓度为诱导生根药剂,做绿枝扦插试验。结果表明:适宜欧李绿枝扦插的基质为洁净的河沙和细炉灰,适宜的ABT1号生根粉使用浓度为1 000 mg/L。以洁净的河沙基质与ABT1号生根粉1 000 mg/L组合处理欧李绿枝扦插生根率、生根数及根长最高;细炉灰基质与ABT1号生根粉1 000 mg/L组合效果次之。     

强力生根粉在辣椒穴盘育苗上的应用效果研究

以辣椒、强力生根粉为试材,以相对湿度为50%的无土基质为基本原料,按1kg分别添加5、10、15、20mg强力生根粉处理育苗基质,研究强力生根粉对辣椒穴盘育苗效果的影响。结果表明:无土育苗基质中添加一定量的强力生根粉,对辣椒幼苗生长有促进作用,其中以1kg无土育苗基质添加10mg强力生根粉的处理对辣椒幼苗的生长势及根系活力有最好的促进作用,其它处理与对照虽有差异,但不显著。     

9个基因在桑树硬枝扦插生根过程中的表达特性分析

桑树扦插生根涉及复杂的生物学过程,受多种内源激素和环境因素的调节。为发掘桑树扦插生根相关基因,为桑树扦插不定根的形成和调节提供分子理论依据,采用桑品种育711号1年生硬枝为插穗,分别以传统愈伤组织生根技术和新的可诱导皮部生根的技术进行处理,以清水处理为对照,利用qRT-PCR技术检测PPO1、PPO2、PPO3、HSP83A、ILL5、GH3.1、SAUR2、Cacybp、ANT1 9个基因在愈伤组织生根和皮部生根2种生根形式生根过程中的表达变化。结果显示在扦插生根的不同发育阶段,9个基因在皮部生根处理组的表达量均明显高于愈伤组织生根处理组和对照组,且各个基因表达量在不同形式的生根过程中表现出不同的变化趋势,初步推测这些基因在不定根的形成中均发挥了重要作用。研究结果将丰富桑树甚至其他林木扦插生根机制的内容,并可为进一步开发高效实用的桑树扦插技术奠定良好的理论基础。     

Effect of glucose on in vitro rooting of mature plants of Bambusa nutans

A high-frequency in vitro rooting method was developed for Bambusa nutans, one of the economically important bamboos in India. Two clones of approximately 10-year-old B. nutans were used for axillary bud proliferation. The effect of carbohydrate source (glucose and sucrose) and auxins (IAA, IBA and NAA) on the in vitro rooting response was evaluated. Rooting percent, mean number of roots and root length were recorded after 14 days of treatment. Successful treatment (49.0 μM IBA and 88 mM glucose in the induction phase for 3 days followed by MS salts with 88 mM sucrose) was repeated four times each with 100 shoots to ascertain the practical utility of the protocol. Addition of glucose along with 49.0 μM IBA during the root induction phase gave 85% rooting success. Anatomical studies in the nodal regions were conducted to determine the effect of glucose on root primordial development and elongation. It was observed that the presence of glucose in the root induction medium is required to recruit more number of root initials. A simple protocol was developed for the large-scale production of B. nutans, where rooting of microshoots developed from mature tissues is always difficult. This study showed that glucose as carbon source and auxin type IBA is essential for in vitro root formation in the cultures raised from mature tissues.     

Relationship between Deep Root Distribution and Root Penetration Capacity Estimated by Pot Experiments with a Paraffin and Vaseline Layer for Landraces and Recent Cultivars of Wheat

Root growth into deep soil is an important factor for stable production in wheat under drought conditions. Root penetrating capacity (RP) shown by pot experiments with a paraffin-Vaseline layer (PV layer) may be a useful indicator estimating deep rooting ability of wheat genotypes. Previously, we identified genotypes of durum wheat (Triticum turgidum L. var. durum) and bread wheat (T. aestivum L.) with diverse RP by the pot experiments. In this study, we investigated the root distribution of three Ethiopian landraces of durum wheat with high RP, three recent cultivars of durum wheat with low RP and one Japanese cultivar of bread wheat ‘Haruyutaka’ with low RP using: (1) pots with a PV layer, (2) root boxes, (3) artificial field and (4) a normal field to analyze the relationship between RP estimated by pot experiment and root development in the field. In the pot experiments, RP was evaluated by the number of roots penetrating through the PV layer (NRP). In the root-box and field experiments, the root distribution was evaluated by the number of roots on the vertical surface of soil as the root frequency (RF: root number cm-¹ soil surface). Ethiopian landraces had a significantly larger NRP than recent cultivars in the pot experiment. The root box and field experiments showed that Ethiopian landraces tended to have a higher RF than recent cultivars in deep soil layer. We concluded that RP estimated by pot experiments with a PV layer is a useful indicator of deep rooting ability under field conditions.     

三种外源生长素对伞房决明一年生硬枝扦插生根的效应

试验用不同质量浓度的IBA、IAA和NAA溶液处理伞房决明的1年生硬枝。结果表明,①不同质量浓度的外源生长素促根效果不同。IBA的6种浓度处理的促根效果都比对照好;IAA的各种处理效果不明显;NAA 25 mg.L-1的促根效果比对照好,其它浓度处理不如对照好。②在供试的3种外源生长素中,以吲哚丁酸的促根效果最好,其中1 000 mg.L-1处理5 min,500 mg.L-1处理10 min,250 mg.L-1处理30 min和100 mg.L-1处理120 min的促根效果都很突出。     

巨桉组织培养及工厂化育苗技术的研究

巨桉是造纸和人造板的良好材料,其组织培养微繁殖体系已经研究成功。取优树主干基部萌芽或扦插苗1~1.5 cm长的茎段做为外植体,外植体和增殖培养在附加有BAP和NAA的MW培养基上进行。培养条件为26±2℃,日光灯光照10 h.d-1。增殖培养采用BAP 0.1~0.5 mg.L-1和NAA 0.01~0.1 mg.L-1。离体的茎芽适合在B2附加0.5 mg.L-1IBA生根。小苗移栽红心土上,在简易温室成活率高达93%。