Variation of the genomic proportion of the recurrent parent in BC1 and its relation to yield performance in sorghum (Sorghum bicolor) breeding for low-input conditions

In order to define the variation of the genomic proportion of the recurrent parent [G(RP)] and its relation to yield, G(RP) of individual BC₁ plants of two sorghum populations composed of a high‐yielding cultivar as recurrent parent (RP) and a donor with superior drought resistance or grain quality, respectively, was estimated using AFLPs and SSRs. G(RP) in BC₁ ranged from 0.53 to 0.95 and averaged to 0.76 in the population (NP4453 × ‘SV‐2’) × ‘SV‐2’. G(RP) varied between 0.60 and 0.86 and averaged to 0.74 in the BC₁ of (ICV‐219 × ‘SV‐2’) × ‘SV‐2’. Results show that plants with a G(RP) equivalent to BC₂ (0.875) or BC₃ (0.938), respectively, can be selected from BC₁. Yield performance of BC₁S₁ families was tested in field trials carried out in South Africa. The correlation between yield and G(RP) in BC₁ was low. Selection according to G(RP) did not result in an effective preselection for yield.     

p38MAPK expression and apoptosis in the rat unilateral ureteral obstruction model

AIM: To investigate the expression of p38 mitogen-activated protein kinase (p38MAPK) in the kidney after unilateral ureteral obstruction (UUO) in rats and the functional role of it on apoptosis and fibrosis.METHODS: Eighteen Wistar rats underwent UUO were killed at 3, 7, 14 days. Additional 7 rats were sham operated. Histological changes were observed by HE and Masson staining. Immunohistochemistry study was performed on renal tissue for proliferating cell nuclear antigen (PCNA). Apoptotic cells were determined by terminal deoxynucleotidyl transferase- mediated dUTP nick end labeling (TUNEL) and the electrophoresis analysis of genomic DNA. Western blotting of cysteinyl aspartate specific proteinase-3 (caspase-3), p38MAPK and p-p38MAPK were measured.RESULTS: UUO induced a significant increase in renal tubular and interstitial cell apoptosis, immunohistochemistry of PCNA and Western blotting of caspase-3, p-p38MAPK as well as severe morphology changes. However, there was no significant difference between UUO and the control in Western blotting of p38MAPK.CONCLUSION: An in vivo model of renal fibrosis after UUO demonstrates that activated or phosphorylated p38MAPK plays a role in apoptosis of renal tubulointerstitial cells.     

朱顶红无菌苗叶片高效再生体系

以朱顶红（Hippeastrum vittatum）叶片为外植体进行离体培养,具有取材方便、试材充足、成本低等优势,但叶片诱导再生率极低,是朱顶红离体培养的一大难题。本试验中分别以‘花孔雀’和‘黑天鹅’朱顶红无菌苗叶片为外植体,探究了不同植物生长调节剂和不同取材部位对不定芽诱导和继代增殖的影响。结果表明：最佳外植体为 MS 培养基中培养 10 d 形成的幼嫩叶片基部（0.5 cm）,在光照 16 h · d-1（光照强度 36 μmol · m-2 · s-1）下,不定芽诱导的最适培养基为 MS + 2 mg · L-1 6-BA + 1 mg · L-1 NAA + 2 mg · L-1 TDZ,两个品种的不定芽均以间接途径发生,其中‘花孔雀’在培养 40 d 后形成愈伤组织,55 d形成不定芽,诱导率可达 69.44%;‘黑天鹅’在培养 45 d 后形成愈伤组织,65 d 形成不定芽,诱导率达到 66.67%;最适体细胞胚诱导培养基为 MS + 2 mg · L-1 6-BA + 1 mg · L-1 PIC,‘花孔雀’和‘黑天鹅’的诱导率分别达到 66.67%和 63.89%;最佳不定芽增殖培养基为 MS + 2 mg · L-1 6-BA + 1 mg · L-1 NAA + 1 mg · L-1 TDZ,‘花孔雀’和‘黑天鹅’的增殖系数分别达到 4.67 和 3.46;在不添加植物生长调节剂的 MS培养基中进行生根培养,30 d 后两个品种的生根率均达到 100%;将生根培养 30 d 的小植株转移至室温条件下放置 3 d,摘去封口膜再驯化 3 d 后,移栽至经高温消毒的草炭︰蛭石（体积比）为 1︰1 的基质中,成活率达到 100%     

时滞recurrent神经网络模型的全局渐近稳定性

讨论了时滞recurrent神经网络模型的全局渐近稳定性,通过构造适当的Lyapuov函数,利用线性矩阵不等式,给出了一类常时滞recurrent神经网络的新的充分条件,所获的稳定性条件是时滞相关的,稳定性判别条件更宽松.最后通过一个实例说明方法的可行性.     

Genetic characterization of 2 novel feline caliciviruses isolated from cats with idiopathic lower urinary tract disease

Feline caliciviruses (FCVs) are potential etiologic agents in feline idiopathic lower urinary tract disease (I-LUTD). By means of a modified virus isolation method, we examined urine obtained from 28 male and female cats with nonobstructive I-LUTD, 12 male cats with obstructive I-LUTD, and 18 clinically healthy male and female cats. All cats had been routinely vaccinated for FCV. Two FCVs were isolated; I (FCV-U1) from a female cat with nonobstructive I-LUTD, and another (FCV-U2) from a male cat with obstructive I-LUTD. To determine the genetic relationship of FCV-U1 and FCV-U2 to other FCVs. capsid protein gene RNA was reverse transcribed into cDNA, amplified, and sequenced. Multiple amino acid sequence alignments and phylogenetic trees were constructed for the entire capsid protein, hypervariable region E, and the more conserved (nonhypervariable) regions A, B, D, and F. When compared to 23 other FCV isolates with known biotypes, the overall amino acid sequence identity of the capsid protein of FCV-U1 and FCV-U2 ranged from 83 to 96%; identity of hypervariable regions C and E ranged from 58 to 85%. Phylogenetically, FCV-U1 clearly separated from other FCV strains in phenograms based on nonhypervariable regions. In contrast, FCV-U2 consistently segregated with the Urbana strain in all phenograms. Clustering of isolates by geographic origin was most apparent in phenograms based on nonhypervariable regions. No clustering of isolates by biotype was apparent in any phenograms. Our results indicate that FCV-UI and FCV-U2 are genetically distinct from other known vaccine and field strains of FCV.