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21.
在简化牛卵母细胞体外成熟的基础上,观察了保护剂、平衡温度、预平衡方法、解冻方法以及冷冻方法对牛卵泡卵母细胞冷冻的影响。结果表明:在体外成熟培养液中添加26.2 mmol/L的NaHCO3和对屠宰场卵巢进行选择更能促进牛卵母细胞的体外成熟;无论是程序冷冻还是玻璃化冷冻,乙二醇(EG)与甘油(GLY)相比更能促进牛卵泡卵母细胞的冷冻效果;在程序冷冻中,冷冻液中添加0.1 mol/L蔗糖比添加0.3mol/L的蔗糖更能促进牛卵泡卵母细胞的冷冻;在玻璃化冷冻中,37℃和25℃的平衡温度比4℃更适合牛卵泡卵母细胞的冷冻;在10%、5%、1%的预平衡浓度之间,5%的预平衡浓度即可达到预平衡的效果;在解冻时,多步脱除保护剂更能保护冷冻的卵母细胞;比较了几种最小样本量(minimum size sample,MSS)玻璃化冷冻方法,解冻成熟培养后的成熟率,拉细毛细玻璃管冷冻法为(41.67±3.19)%,极显著高于未拉细毛细玻璃管冷冻法(glassmicropipette,GMP)的(30.19±1.93)%和固体表面冷冻法(solid surface vitrification,SSV)的(28.33±2.89)%(P<0.01)。 相似文献
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本试验选用MRS、MRS+10%家兔硬粪浸出液和MRS+10%泡菜汁3种选择培养基,对健康家兔肠道的乳酸菌群分别进行需氧和厌氧培养,根据可培养细菌菌落特征、染色特性、显微镜形态观测,共分离出7株乳酸菌株,其中需氧菌2株,厌氧菌5株。结合生化试验初步鉴定,需氧菌株分别为乳酸乳球菌和短乳杆菌,厌氧菌株分别为嗜酸乳杆菌,嗜粪乳杆菌,肠乳杆菌,乳酸乳杆菌和弯曲乳杆菌。本实验为弄清健康家兔肠道的有益菌群,以便下一步制作更易于在畜禽的肠道中定植存活的复合动物微生态制剂做准备。 相似文献
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Szalo IM Lassence C Licois D Coudert P Poulipoulis A Vindevogel H Marlier D 《Veterinary journal (London, England : 1997)》2007,173(3):652-657
Epizootic rabbit enteropathy (ERE) is a major cause of economic loss in intensive rabbit production. Since its first recognition in 1997, much work has been done to determine the pathogenic mechanisms of the disease and to identify the aetiological agent(s). Unfortunately, the quest for aetiology has only met with limited success despite the ability to reproduce the syndrome by inoculation of intestinal contents from field cases. These intestinal inocula contain a huge number of microorganisms which could all be involved in the aetiology of ERE. To decrease the number of putative agents, the French reference inoculum TEC3 was fractionated on a discontinuous sucrose gradient so that seven fractions (supernatant, 10%, 20%, 30%, 40%, 50% and pellet) were obtained. Specific-pathogen-free rabbits were inoculated with three out of these seven fractions (supernatant, 30%, and pellet). The objectives were: (1) to characterise the seven fractions by bacteriological examination; (2) to verify whether the aetiological agent was present in the fractions by inoculation of rabbits; (3) to assign the aetiological agent of ERE to a morphological group of pathogens; (4) to identify a fraction which could replace the reference inoculum TEC3 in applications such as cell cultures or egg inoculation. The results strongly suggest that ERE is a bacterial disease and does not have a viral or parasitic aetiology. 相似文献
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比较了水牛卵母细胞体外成熟时间对孤雌激活胚发育能力的影响,以及不同化学激活方法对水牛卵母细胞孤雌激活效果的影响,并在相同条件下,对孤雌激活胚与体外受精胚的发育能力进行了比较.结果表明,水牛卵母细胞体外成熟27 h或30 h的囊胚发育率(19.0%、17.7%)明显高于体外成熟21 h或24h的囊胚发育率(12.3%、13.8%);Ion联合6-DMAP激活水牛卵母细胞的效果优于其他几组激活方法;在相同条件下,孤雌激活胚与体外受精胚的发育能力存在着差异,其中卵裂率差异不显著,但孤雌激活胚的囊胚发育率显著高于体外受精胚. 相似文献
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XIAO Chao-neng WENG Ji-mei LI Wei JIANG Zhao-hua LIN Jia-dong LIU Ruo-yu LIN Hong-lin ZU Pan-yu ZHANG Fu-ping 《中国畜牧兽医》2017,44(8):2378-2385
This study was aimed to analyze the association of insulin-like growth factor 1 (IGF1) gene polymorphisms with growth traits in rabbit. The technology of DNA pooling, PCR-SSCP and direct sequencing were used to detect the SNP of IGF1 gene. The results showed that T365C was detected in IGF1 gene exon 3 of rabbit. The SNP (C/T) was devised into three genotypes:TT, TC and CC. The relationships between genotypes and growth traits revealed that the birth weight and the weight at 28 days of age in New Zealand rabbit of TT genotype of T365C locus were significantly higher than TC and CC genotypes (P<0.05), the birth weight, the weight at 90 days of age and average daily gain form birth to 90 days of age in Belgian rabbit of TT genotype of T365C locus were significantly higher than TC and CC genotypes (P<0.05), there were no significant difference of the growth traits in other rabbit breeds (P>0.05).The consequence indicated that IGF1 gene was primarily deduced to be a potential major gene or linked to major gene effecting the growth traits of rabbit, and this SNP (T365C) might be a candidate molecular genetic markers to improve the growth traits of rabbit. 相似文献
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为建立新西兰兔前体脂肪细胞的体外培养模型,比较新西兰兔肌内和皮下前体脂肪细胞分化过程中相关基因的差异表达,实验采集 1 日龄新西兰兔背最长肌和皮下脂肪组织,采用胶原酶消化方法,分别从 2 种组织中分离前体脂肪细胞,进行细胞原代培养,绘制细胞生长曲线,并对肌内和皮下前体脂肪细胞诱导分化,利用 RT-PCR 检测相关基因的表达变化。结果表明:细胞在分离后 2 h 已经贴壁,24 h 时呈现出短梭形的细胞形态,第 2 天细胞变成长梭形,第 3 天进入对数生长期。肌内和皮下前体脂肪细胞在诱导分化后均被油红O 染色,皮下前体脂肪细胞的脂质积累在诱导分化的第 2 天显著高于肌内前体脂肪细胞(P<0.05);荧光定量结果表明,肌内和皮下前体脂肪细胞 CCAAT 增强子结合蛋白(C/EBPα)基因的表达趋势在诱导分化过程中相同,肌内前体脂肪细胞过氧化物酶体增殖激活受体(PPARγ)第 4 天的表达量显著高于第 6 天,而皮下前体脂肪细胞 PPARγ表达量差异不显著;肌内前体脂肪细胞脂蛋白脂肪酶(LPL)表达量在第 0天和第 2天差异不显著,而皮下前体脂肪细胞第 2 天显著高于第 0 天(P<0.05);肌内前体脂肪细胞脂肪酸合酶(FAS)基因第 0、2、4 天的表达量差异不显著,而皮下前体脂肪细胞第 2、4 天显著高于第 0 天(P<0.05)。本研究成功构建了新西兰兔肌内和皮下前体脂肪细胞的体外培养和诱导分化模型,并发现皮下前体脂肪细胞分化早于肌内前体脂肪细胞,为进一步研究新西兰兔前体脂肪细胞的分化机制和脂肪沉积奠定基础。 相似文献
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F. Bovera S. D’Urso C. Di Meo R. Tudisco A. Nizza 《Journal of animal physiology and animal nutrition》2009,93(2):147-156
An in vitro gas production trial was conducted using 10 Hyla rabbits to evaluate the use of caecal and faecal inocula in fermentability studies. Caecal content (CI) and hard faeces (FI) were used as inocula. Six legume and six cereal concentrates were used as substrates. Gas production was recorded 19 times at 2–24 h intervals throughout fermentation (120 h). The fermentation characteristics (degraded organic matter, OMd; potential gas production, A; volatile fatty acid, VFA; ammonia, NH3) were measured by testing the inoculum and substrate (legumes vs. cereals). The inocula were different (p < 0.01) in OMd (818.2 vs. 799.4 g/kg OM for CI and FI respectively), A (255.0 vs. 267.1 ml/g OM), total VFA (67.3 vs. 53.2 mmol/g OM) and NH3 content (21.2 vs. 19.8 mmol/l). Moreover, the significant differences in the (acetate + propionate)/butyrate ratio (5.02 vs. 4.09 for CI and FI respectively, p < 0.01) and in the branched chain proportion (isobutyrate + isovaleriate)/total VFA (0.044 vs. 0.031, p < 0.01) indicate that the inocula differed in fermenting legumes or cereals, but the equations for estimating caecal fermentation characteristics from those of faeces showed R2 values from 0.673 to 0.975 (p ≤ 0.01). Our results confirm that in vitro fermentation characteristics of faeces were highly related to those of caecal content. 相似文献
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对猪体细胞克隆技术中关键步骤之一的去核方法作了较深入的研究。结果表明,由本实验室改进的Willadsen去核法——二步挤压去核法,无论在去核操作成功率(73.9%)上还是在去核效率(81.2%)上都明显好于McGrath-Solter去核法(42.5%、67.4%,P〈0.05);同时,本试验还发现,卵母细胞成熟培养36h后去核组去核效率(89.1%)显著地高于成熟培养44h后去核组(55.8%,P〈0.05),而核移植重构胚的发育率2个组间无显著差异。 相似文献