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591.
Kayoko Kudo Toru Hoshiya Tomomi Nakazawa Tsubasa Saito Natsumi Shimoyama Isamu Suzuki Kazutoshi Tamura John Curtis Seely 《Journal of toxicologic pathology》2012,25(4):277-280
Spontaneous renal tubule tumors (RTTs), with a distinctive morphological phenotype, were
present in three Sprague-Dawley rats, 1 male and 2 females, out a total of 120 animals of each
sex from untreated and placebo control groups in a 2-year carcinogenicity study. One female had
one carcinoma, adenoma and hyperplasia, and the other female had five adenomas and many
hyperplastic lesions; the male case had one carcinoma. From these cases, a biological continuum
of hyperplasia, adenoma and carcinoma could be recognized. The tumors were present in the renal
cortex and appeared as solid lobulated growths with occasional central necrosis. The lobules
were divided by a small amount of fibrovascular tissue. Occasionally the larger tumors
contained a cystic area. Tumor cells appeared distinctive and exhibited variable amounts of
eosinophilic/amphophilic and vacuolated cytoplasm. Nuclei were round to oval with a prominent
nucleolus. Mitotic figures were uncommon, and no distant metastasis was noted. The tumors were
seen as multiple and bilateral lesions in two animals and had no apparent relationship to
chronic progressive nephropathy (CPN). Foci of tubule hyperplasia were also noted to contain
the same type of cellular morphology. The morphological and biological features of these 3
cases resembled the amphophilic-vacuolar (AV) variant of RTT that has been posited to be of
familial origin. This is a report of spontaneous familial renal tumors in Sprague-Dawley rats
from Japan. 相似文献
592.
Canine bicavitary carcinomatosis with transient needle tract metastasis diagnosed by multiplex immunocytochemistry 下载免费PDF全文
A Russell Moore Emily Coffey Sarah E. Leavell Greta Krafsur Colleen Duncan Kristy Dowers Kelly S. Santangelo 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(3):495-500
A 6‐year‐old, male castrated, mixed‐breed dog was referred to the James L. Voss Veterinary Teaching Hospital at Colorado State University for bicavitary effusion. On examination, the dog was tachycardic and tachypneic with bilaterally decreased lung sounds. Thoracic and abdominal ultrasonic examination revealed pleural and peritoneal effusions, which were aspirated and submitted for fluid analysis and cytology. Both cavity fluids were classified as exudates with a large population of vacuolated mononuclear cells. Multiplex immunocytochemistry (ICC) for cytokeratin and vimentin demonstrated exclusively cytokeratin expression, indicating these cells were of epithelial origin. A full diagnostic evaluation was performed, including CBC, clinical chemistry, a pet‐side test for heartworm disease, ehrlichiosis, Lyme disease, and anaplasmosis, imaging modalities of thorax, abdomen, and heart, urinalysis, and fine‐needle aspirations of spleen, liver, and popliteal lymph nodes. The dog was diagnosed with pleural and peritoneal carcinoma with presumed carcinomatosis. A single dose of intracavitary carboplatin was administered before discharge, and over a period of 2 weeks, 5 thoracocenteses were performed. A subcutaneous mass was noted at a thoracocentesis site one week after initial presentation. Cytology of the mass was consistent with carcinoma, and neoplastic seeding of the tumor cells from the thoracocentesis was suspected. The dog was euthanized 15 days after the first visit, and a necropsy was performed. Findings were consistent with carcinomatosis secondary to anaplastic pulmonary carcinoma with transient subcutaneous seeding of neoplastic cells during routine thoracocentesis. This case demonstrates the utility of multiplex ICC in the clinical setting. 相似文献
593.
Treatment of corneal squamous cell carcinoma using topical 1% 5‐fluorouracil as monotherapy 下载免费PDF全文
Daniel M. Dorbandt Elizabeth A. Driskell Ralph E. Hamor 《Veterinary ophthalmology》2016,19(3):256-261
The purpose of this report is to discuss the use of topical 1% 5‐fluorouracil as a sole therapy for canine corneal squamous cell carcinoma (SCC). A 12‐year‐old castrated male pug was evaluated for a well‐demarcated, central, 3 mm in diameter, pale pink, raised, right corneal mass. An incisional biopsy was obtained using a #64 beaver blade after topical anesthesia and without sedation. A definitive diagnosis of corneal SCC was obtained after histopathologic evaluation of the biopsy. Topical 1% 5‐fluorouracil ointment was applied to the right eye four times daily for 2 weeks followed by no treatment for 2 weeks, then treatment again twice daily for 2 weeks. The cornea remained free of recurrence 10 months after cessation of treatment. In dogs affected with corneal SCC, topical 1% 5‐fluorouracil monotherapy may be a viable and cost‐effective treatment option with minimal side effects. This chemotherapy agent may also have an effect on corneal pigmentation. Chronic cyclosporine therapy did not contribute to the pathogenesis of corneal SCC in the case described. 相似文献
594.
ZHANG Tong ZENG Xian-cheng LI Hua CHEN Wen-jie FANG Tian-ling NIE Chang-fu CHEN Gui-hua 《园艺学报》2012,28(10):1884-1886
AIM: To investigate the human leukcyte antigen E (HLA-E) expression in hepatocellular carcinoma cell lines. METHODS: The techniques of real-time PCR and Western blotting were used to study the HLA-E expression in the 5 cell lines of hepatocellular carcinoma and a fetal liver cell line at mRNA and protein levels. RESULTS: The results of real-time PCR showed that no statistical difference of HLA-E mRNA level between fetal liver cell L02 and other 4 cell lines of hepatocellular carcinoma (HepG2, Bel7402, PLC and MHCC97) was observed, and almost absence of HLA-E mRNA expression in Hep3B2.1-7 cells was detected. However, the results of Western blotting showed that there was a significant statistical difference of HLA-E protein levels between L02 cells and the 5 cell lines of hepatocellular carcinoma (HepG2, Bel7402, PLC, MHCC97 and Hep3B2.1-7), and no HLA-E protein in Hep3B2.1-7 cells was detectable. CONCLUSION: Asynchronization of HLA-E expression between mRNA and protein levels was found in hepatocellular carcinoma cell lines. 相似文献
595.
596.
597.
M.R. Broome M.E. Peterson J.R. Walker 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(5):1560-1568
Background
Thyroid neoplasia is common in dogs, but there are few reports of dogs with ectopic, sublingual thyroid tumors.Objectives
To describe clinical features and outcomes of dogs with ectopic, sublingual thyroid neoplasia.Animals
Five hundred and forty‐four dogs with thyroid neoplasia.Methods
This retrospective study reviewed the medical records of dogs referred for thyroid neoplasia between 1995 and 2013. Data extracted included signalment, extent of thyroid disease (eutopic or ectopic; metastasis), serum thyroxine (T4) concentration, treatment, and survival.Results
Of 544 dogs with thyroid neoplasia, 41 (7.5%) dogs had ectopic sublingual thyroid tumors. The clinical features of these 41 dogs were similar to the cohort group of 503 dogs with eutopic or ectopic mediastinal thyroid tumors, but dogs with sublingual tumors were younger and less likely to have metastatic disease (15% versus 30%, P < .05). Of the 41 dogs, 28 received treatment: 21 with surgery (which included partial hyoidectomy in 13), 7 with radioiodine alone, and 13 with surgery followed by administration of radioiodine. Overall median survival was 562 days (range, 1‐1,850 days).Conclusions and Clinical Importance
When compared with eutopic thyroid carcinomas, ectopic sublingual thyroid tumors generally have a less aggressive biologic behavior. Many dogs have prolonged survival, even without treatment, although death because of local tumor invasiveness or metastasis can develop in some dogs. Surgical thyroidectomy, including partial hyoidectomy, is generally effective for control of local disease. Administration of radioiodine, alone or in combination with surgical treatment, is recommended for multifocal disease or metastasis. 相似文献598.
WANG Si-si LIAO Xiao-min SHAO Zhong-ming YUAN Jian-ling FENG Mu-yin HA Yan-ping LI Ru-jia SHEN Zhi-hua JIE Wei 《园艺学报》2018,34(12):2172-2179
AIM:To establish SETD2 gene knockout nasopharyngeal carcinoma (NPC) cell strains based on CRIPSR/Cas9 technique and to analyze their proliferation characteristics. METHODS:Sub-quantitative RT-PCR and Western blot were used to detect the expression of SETD2 in immortalized nasopharyngeal epithelial cell line NP-69, well differentiated NPC cell line CNE1, poorly-differentiated NPC cell line CNE2Z and undifferentiated NPC cell line C666-1, and the SETD2 high expression cell line CNE1 was screened. The proliferation ability of CNE1 cells before and after the SETD2 gene knockout was analyzed by CCK-8 and colony formation assay. The cell cycle distribution was detected by flow cytometry, and the expression of cell cycle-related proteins was detected by Western blot. RESULTS:Compared with NP-69 cells, the expression of SETD2 was decreased gradually in CNE1, CNE2Z and C666-1 cells (P<0.01). Based on the CRISPR/Cas9 technique, 2 monoclonal cell strains with SETD2 gene stable knockout, named CNE1-SETD2-KO-#5 and #9, were successfully screened from total 15 monoclones. The results of CCK-8 and plate colony formation assay confirmed that the proliferation ability of CNE1-SETD2-KO-#5 and #9 cells was significantly enhanced compared with CNE1-WT cells (P<0.05). The results of flow cytometry analysis showed that the G1 phase of CNE1-SETD2-KO-#5 and #9 cells was decreased, while the G2/M and S phases were increased significantly (P<0.05). The results of Western blot confirmed the increases in the protein levels of proliferating cell nuclear antigen (PCNA), cyclin D1, cyclin B1, cyclin A2, cyclin E1, cyclin-dependent kinases 2 (CDK2) and CDK4, and the decrease in the protein level of p21 after SETD2 gene knockout (P<0.05). CONCLUSION:The NPC cell strains with SETD2 gene knockout were successfully constructed based on CRISPR/Cas9 technique. SETD2 expression correlates with cell differentiation status in the NPC cells. SETD2 gene knockout promotes NPC cell proliferation by up-regulating cyclin D1, cyclin B1, cyclin A2, cyclin E1, CDK2 and CDK4, and down-regulating p21 expression. 相似文献
599.
Abstract This report describes clinical, histological and post-mortem findings in a cat, that are similar to necrolytic migratory erythema in humans and to metabolic epidermal necrosis in dogs.
Resumen Este articulo describe los hallazgos clinicos, histopatológicos y post mortem en un gato, similares a Eritema Necrolitico Migratorio en la especie humana y a Necrosis Epidérmica Metabólica en el perro. [Patel, A., Whitbread, J., McNeil. P. A case of metabolic epidermal necrosis in a cat. (Un caso de necrosis epidérmica en un gato.) Veterinary Dermatology 1996; 7 : 221–226.]
Résumé Ce cas décrit les lésions cliniques, histopathologiques et nécropsiques chez un chat, évoquant l'érythème nécrolytique migrant de l'homme et la nécrose épidermique métabolique du chien. [Patel, A., Whitbread, J., McNeil. P. A case of metabolic epidermal necrosis in a cat. (Un cas de nécrose épidermique métabolique chez un chat.) Veterinary Dermatology 1996; 7 : 221–226.]
Zusammenfassung Dieser Bericht beschreibt klinische, histopathologische und Sektionsbefunde bei einer Katze, die dem hekrolytischen migratorischen Erythem beim Menschen und der stoffwechselbedingten epidermalen Nekrose beim Hund ähneln. [Patel, A., Whitbread, J., McNeil. P. A case of metabolic epidermal necrosis in a cat. (Ein Fall von stoffwechselbedingter epidermaler nekrose bei der Katze) Veterinary Dermatology 1996; 7 : 221–226.] 相似文献
Resumen Este articulo describe los hallazgos clinicos, histopatológicos y post mortem en un gato, similares a Eritema Necrolitico Migratorio en la especie humana y a Necrosis Epidérmica Metabólica en el perro. [Patel, A., Whitbread, J., McNeil. P. A case of metabolic epidermal necrosis in a cat. (Un caso de necrosis epidérmica en un gato.) Veterinary Dermatology 1996; 7 : 221–226.]
Résumé Ce cas décrit les lésions cliniques, histopathologiques et nécropsiques chez un chat, évoquant l'érythème nécrolytique migrant de l'homme et la nécrose épidermique métabolique du chien. [Patel, A., Whitbread, J., McNeil. P. A case of metabolic epidermal necrosis in a cat. (Un cas de nécrose épidermique métabolique chez un chat.) Veterinary Dermatology 1996; 7 : 221–226.]
Zusammenfassung Dieser Bericht beschreibt klinische, histopathologische und Sektionsbefunde bei einer Katze, die dem hekrolytischen migratorischen Erythem beim Menschen und der stoffwechselbedingten epidermalen Nekrose beim Hund ähneln. [Patel, A., Whitbread, J., McNeil. P. A case of metabolic epidermal necrosis in a cat. (Ein Fall von stoffwechselbedingter epidermaler nekrose bei der Katze) Veterinary Dermatology 1996; 7 : 221–226.] 相似文献
600.
Calponin expression and myoepithelial cell differentiation in canine, feline and human mammary simple carcinomas 总被引:1,自引:0,他引:1
J. Martín de las Mulas C. Reymundo A. Espinosa de los Monteros Y. Millán J. Ordás 《Veterinary and comparative oncology》2004,2(1):24-35
Calponin is a 34‐kDa smooth muscle‐specific protein that has been shown to be a highly sensitive marker of myoepithelial cells in canine, feline and human mammary tissue and tumours. The expression of calponin was studied in 15 canine, 32 feline and 28 human simple mammary carcinomas using a monoclonal mouse antihuman calponin antibody and the avidin–biotin peroxidase complex (ABC) immunohistochemical technique. Calponin expression was compared with the expression of cytokeratin 14, a marker of normal mammary myoepithelial cells in the three species. Four different types of calponin‐positive cells were identified: (1) Type 1: cytokeratin‐14‐positive pre‐existing myoepithelial cells forming a continuous layer with images of focal disruptions; (2) Type 2: cytokeratin‐14‐positive isolated nests of fusiform, polygonal or round cells without atypia; (3) Type 3: cytokeratin‐14‐positive atypical cells indistinguishable from non‐reactive atypical cells, which should have never been detected in haematoxylin and eosin‐stained sections and (4) Type 4: cytokeratin‐14‐negative stromal fusiform cells around the neoplastic growth or cell nests, identified as myofibroblasts. Calponin‐negative and cytokeratin‐14‐positive atypical neoplastic cells were observed in three canine, 28 feline and two human carcinomas. The latter were indicative of altered expression of high‐molecular‐weight cytokeratins in luminal epithelial‐type simple carcinomas. Our findings show that calponin is a good marker of myoepithelial cell differentiation in feline, human and, particularly, canine simple carcinomas. The high number (six out of 15) of canine tumours with type 3 cells points to the need of both introducing calponin examination in the routine diagnostic schedule and performing further studies on its prognostic significance. 相似文献