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以款冬叶片为试材,采用微波辅助法提取总黄酮,在单因素试验的基础上,采用正交实验法优化款冬叶片总黄酮提取工艺,同时以分光光度法测定提取物对1,1-二苯基-2-三硝基苯肼自由基(1,1-diphenyl-2-picrylhydrazyl,DPPH·)、羟基自由基(·OH)和超氧阴离子自由基(O-·2)的清除作用。结果表明:款冬叶片总黄酮最佳提取条件为乙醇体积分数70%,微波提取时间3 min,微波功率500 W,料液比1∶15 g·mL^-1,在该条件下,总黄酮的提取率为0.91%。款冬叶片总黄酮的质量浓度与抗氧化活性呈一定的线性关系,当总黄酮质量浓度为0.069 8 mg·mL^-1时,对DPPH·、·OH和O-·2的清除率分别达84.21%、84.10%和68.32%,表明款冬叶片具有良好的抗氧化能力,可作为天然的抗氧化剂进一步的开发和利用。 相似文献
66.
内毒素对仔鸡血浆SOD活性及MDA水平的影响 总被引:1,自引:1,他引:0
为了探讨大肠埃希菌O55∶B5的内毒素(LPS)对仔鸡的损伤机理,将168只仔鸡随机分成4组(n=42),即对照组,100 mg/kgLPS组,200 mg/kg LPS组,300 mg/kg LPS组。灌服内毒素后,分别于2,4,8,12,24,48 h和72 h处死鸡(各时间点均为6只),测定血浆SOD活性和MDA浓度。结果表明,与对照组相比,各剂量组鸡血浆SOD活性均明显降低,MDA含量均明显升高(P<0.05)。且各剂量组之间SOD活性随攻毒剂量的增加而降低,随时间的延长先降低后升高;MDA含量随攻毒剂量的增加而升高,随时间的延长先升高后降低。表明一定量内毒素能诱发鸡体内氧自由基的产生,显著降低机体中SOD的活性和提高MDA的含量。 相似文献
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为探讨耕作方式对黑土区农田土壤物理特性的影响,2016年6—9月在内蒙古自治区呼伦贝尔市阿荣旗试验区设置3种耕作方式深松(SS)、免耕(MG)及常规耕作(CK),分别采集0-10 cm、10-20 cm和20-30 cm土层的土样,对土壤容重、含水量、土壤渗透速率及土壤孔隙度进行调查。结果表明:3种耕作方式土壤容重及含水量均表现为SS>CK>MG;土壤入渗特征表现为初始入渗率>平均渗透率>稳定入渗率;土壤入渗速率及累计入渗量表现为SS>CK>MG;土壤孔隙度以SS处理下最高且随着月份的增加土壤孔隙度降低。主成分分析表明,初始入渗速率和渗透总量对不同耕作方式最为敏感,可以作为考察东北黑土区农田耕作效应与缓解黑土地退化的评价指标。由综合得分可知,不同耕作方式下土壤性能优劣表现为SS>MG>CK。综上,深松耕作可以有效的降低土壤容重,提高土壤含水量,促进土壤渗透能力;免耕更有利于水分的保持,降低土壤的无效蒸发。 相似文献
68.
QU Yi-ming SHAO Gao-hai ZHANG Ming-hua LI Bo ZHU Feng-chen HE Chao CAO Chun-feng ZHAO Bo 《园艺学报》2020,36(6):1089-1095
AIM To explore the effect of platelet-rich plasma (PRP) on rabbit osteoarthritis and its possible mechanism. METHODS The rabbits with knee osteoarthritis were prepared and then divided into model group, sodium hyaluronate (SH) group and PRP group, and another sham operation group was set up, with 6 rabbits in each group. The gross morphological changes of rabbit cartilage were observed. HE staining was used to evaluate the pathomorphological changes of the cartilage. TUNEL staining was used to detect the apoptosis of chondrocytes. The expression of nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3)/interleukin-1β (IL-1β) signaling pathway-related molecules was observed by immunohistochemical staining, and the protein levels of caspase-3, Bcl-2 and Bax were determined by Western blot. Chondrocytes were isolated and processed according to grouping, and the NLRP3 and IL-1β levels of the cells were measured by ELISA. RESULTS Compared with sham operation group, Pelletier score, Mankin score, chondrocyte apoptotic rate, the positive protein expression rates of NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), caspase-1 and IL-1β, and the protein levels of caspase-3 and Bax in model group were increased significantly (P <0.05), while the protein expression of Bcl-2 was decreased significantly (P <0.05). Compared with model group, Pelletier score, Mankin score, the apoptotic rate of chondrocytes, the positive protein expression rates of NLRP3, ASC, caspase-1 and IL-1β, and the protein levels of caspase-3 and Bax in SH group and PRP group were decreased significantly (P <0.05), while the protein expression of Bcl-2 was increased significantly (P <0.05). In PRP group, Pelletier score, Mankin score, the apoptotic rate of chondrocytes, the positive protein expression rates of NLRP3, ASC, caspase-1 and IL-1β, and the protein levels of caspase-3 and Bax were lower than those in SH group, while the protein expression of Bcl-2 was higher than that in SH group (P <0.05). Compared with control group, the expression of NL?RP3 and IL-1β in MCC950 (NLRP3 ihibitor) group were significantly reduced (P <0.05), the expression of NLRP3 in eucalyptol (IL-1β inhibitor) group was not significantly changed (P >0.05), and the expression of IL-1β was significantly reduced (P <0.05). CONCLUSION Platelet-rich plasma promotes the repair of cartilage in osteoarthritis rabbits, which has better effect than SH. The mechanism may be related to the inhibition of NLRP3/IL-1β pathway and the reduction of chondrocyte apoptosis. 相似文献
69.
In coping with increasing energy consumption and the consequential environmental pollution, green development is becoming an important part of social development. Since the inauguration of China’s Silk Road Economic Belt Initiative (BRI), economic cooperation between China and other countries along BRI has seen much growth. Therefore, it is of great significance to study the levels of green total factor productivity (GTFP) of the countries in the Silk Roads Economic Belt (B&R) and examine how these levels vary spatially and evolve temporally. In this paper, a panel regression analysis with DEA windows is used to study the spatiotemporal trends of the levels of GTFP. The results are: (1) The B&R countries have seen an increase in their overall levels of GTFP over time; (2) There are regional differences in the levels of GTFP, with higher efficiency in Western Asia and Central and Eastern Europe, and lower efficiency in Southeast and Central Asia; (3) The analysis of GTFP for countries in these regions shows that the efficiencies of most countries have been improving, with that of only a few countries have been decreasing; (4) By using regression analysis, we find that variables such as import, export, industrial structure, and urban affect the B&R countries with growing GTFP. 相似文献
70.
为探究阻燃剂对沙柳液化产物/异氰酸酯泡沫材料导热行为的影响和开发具有阻燃特性的聚合物复合材料,以沙柳多元醇液化产物为原料、辛酸亚锡为催化剂、水为发泡剂,与异氰酸酯进行发泡制备沙柳液化产物/异氰酸酯硬质泡沫,利用正交试验法研究工艺因子对硬质泡沫材料导热性能的影响规律,使用锥形量热仪(CONE)对该材料燃烧行为进行分析。结果表明:1)沙柳液化产物/异氰酸酯泡沫材料制备工艺对导热性能的影响关系为阻燃剂添加量>沙柳液化产物与异氰酸酯比例>阻燃剂种类,方差分析表明工艺因子对导热性能的影响均不显著;2)最优工艺:阻燃剂添加量8%,沙柳液化产物/异氰酸酯比例1.0∶1.0,阻燃剂为有机蒙脱土,制备的硬质泡沫材料密度0.168 g·cm-3,抗压强度0.83 MPa,导热系数0.118 3 W·m-1·K-1,此工艺制备的聚氨酯泡沫是一种优良的保温材料;3)不同有机蒙脱土添加量的沙柳液化产物/异氰酸酯泡沫燃烧行为属于双阶段燃烧过程,阻燃剂有机蒙脱土添加量8%,燃烧开始时间推迟至120 s且总热释放量降低。 相似文献