全文获取类型
收费全文 | 170篇 |
免费 | 18篇 |
国内免费 | 9篇 |
专业分类
林业 | 21篇 |
农学 | 3篇 |
综合类 | 30篇 |
农作物 | 11篇 |
畜牧兽医 | 3篇 |
园艺 | 5篇 |
植物保护 | 124篇 |
出版年
2024年 | 2篇 |
2023年 | 2篇 |
2022年 | 5篇 |
2021年 | 8篇 |
2020年 | 7篇 |
2019年 | 5篇 |
2018年 | 4篇 |
2017年 | 2篇 |
2016年 | 7篇 |
2015年 | 7篇 |
2014年 | 18篇 |
2013年 | 11篇 |
2012年 | 6篇 |
2011年 | 14篇 |
2010年 | 20篇 |
2009年 | 7篇 |
2008年 | 3篇 |
2007年 | 10篇 |
2006年 | 7篇 |
2005年 | 13篇 |
2004年 | 3篇 |
2003年 | 6篇 |
2002年 | 10篇 |
2001年 | 5篇 |
2000年 | 7篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 3篇 |
排序方式: 共有197条查询结果,搜索用时 359 毫秒
51.
云南泡桐丛枝病植原体核糖体蛋白基因片段序列分析 总被引:3,自引:0,他引:3
应用植原体核糖体蛋白基因通用引物对rpF1/rpR1,对采自云南省曲靖市的泡桐丛枝病植原体DNA (PaWB-QJ)进行PCR扩增,得到1.3 kb的特异片段,证明此病株中存在植原体。将此片段与pGEM-T Easy载体连接并转化大肠杆菌JM109感受态细胞,进行PCR鉴定、核糖体蛋白基因部分核苷酸序列测定及分析。结果表明,该株系(PaWB-QJ)核糖体蛋白基因片段长1 244 bp,包含rps19、rpl22和rps3基因。对PaWB-QJ株系的核糖体蛋白基因序列的同源性比较结果显示与16S rI-B亚组的翠菊黄化(Aster yellows,AY)、长春花黄化(Periwinkle yellows,PY)和泡桐丛枝德国株系(Paulownia witches'-broom,PaWB-German)的亲缘关系最近,达到99.0%以上,而与其它组中的株系明显低于97.0%,所以认为该植原体株系属于翠菊黄化组B亚组(16SrI-B)。 相似文献
52.
The possible transovarial transmission of two phytoplasmas, ' Candidatus Phytoplasma mali' and ' Candidatus Phytoplasma prunorum', through their respective psyllid vectors Cacopsylla melanoneura and Cacopsylla pruni , was investigated. Different life stages of the progeny of infected female psyllids were analysed by PCR detection of phytoplasma DNA. While ' Ca. Phytoplasma mali' could not be detected in any of the C. melanoneura life stages tested, ' Ca. Phytoplasma prunorum' could be detected in eggs, nymphs and newly emerged adults of C. pruni . Infectivity tests using both nymphs and newly emerged adults of C. pruni showed that ' Ca. Phytoplasma prunorum' inherited from infected females can be transmitted to healthy plum plants. Although further validations are required, these findings open up new perspectives on the study of the epidemiology of diseases associated with European stone fruit yellows. 相似文献
53.
Detection and identification of a phytoplasma from lucerne with Australian lucerne yellows disease 总被引:2,自引:0,他引:2
L. J. Pilkington † K. S. Gibb G. M. Gurr M. J. Fletcher A. Nikandrow E. Elliott R. van de Ven D. M. Y. Read 《Plant pathology》2003,52(6):754-762
Foliar and root symptoms are described for Australian lucerne yellows (ALuY), a disease common in Australian lucerne seed crops. A phytoplasma was detected in plants exhibiting symptoms, but not in symptomless lucerne plants. Oligonucleotide primers specific to the phytoplasma 16S-23S rRNA intergenic spacer region (SR) were used in polymerase chain reaction (PCR) assays on DNA extracted from lucerne plants with and without symptoms. Identical restriction fragment length polymorphism (RFLP) enzyme profiles were obtained for PCR products amplified from 10 yellows-affected lucerne samples. RFLP profiles obtained for four restriction enzymes were different from those of the tomato big bud (TBB) phytoplasma. ALuY phytoplasma PCR products were sequenced to determine phylogeny and were found to fall within the faba bean phyllody phytoplasma group, or phytoplasma group 16srII. Transmission electron microscopy revealed phytoplasmas in the phloem of yellows-affected plant samples, but not in symptomless plant samples. Fungal, bacterial and viral agents in the aetiology of Australian lucerne yellows were ruled out. 相似文献
54.
Manabu KUSUNOKI Toshiki SHIOMI Motowo KOBAYASHI Torao OKUDAIRA Akihiro OHASHI Teruo NOHIRA 《Journal of General Plant Pathology》2002,68(2):147-154
Phytoplasmas causing a severe decline of three tree species, i.e., Rhus javanica, Hovenia tomentella and Zizyphus jujuba, in Japan were examined for their transmissibility by a leafhopper species Hishimonus sellatus, and for their phylogenetic relatedness. By H. sellatus, Rhus yellows (RhY) phytoplasma was transmissible to white clover and periwinkle seedlings, causing typical symptoms in these
plants. Jujube witches' broom (JWB) phytoplasma was also transferred to the host plant, Z. jujuba, by the leafhopper. Because JWB phytoplasma was transmitted to Hovenia tomentella and caused the same symptoms as Hovenia witches' broom (HWB), JWB phytoplasma may be very closely related to HWB phytoplasma.
RFLP analysis of the PCR products of 16S rDNA revealed that RhY phytoplasma belongs to the Aster yellows (AY) group, and JWB
and HWB phytoplasmas belong to a different group (possibly Elm yellows group). Thus, we found that one species of leafhopper
can carry phylogenetically distant phytoplasmas.
Received 23 April 2001/ Accepted in revised form 29 October 2001 相似文献
55.
56.
Hitoshi Nakamura Shigeto Ohgake Norio Sahashi Nobuyuki Yoshikawa Takanori Kubono Tsuyoshi Takahashi 《Journal of Forest Research》1998,3(1):39-42
Seasonal variation of paulownia witches’-broom (PWB) phytoplasma within different organs (leaves, branch and trunk bark and
roots) in paulownia trees was investigated by the amplification of a PWB-specific DNA fragment by the polymerase chain reaction
(PCR). In leaf samples, PWB phytoplasma was first detected in June and the incidence gradually increased. On the other hand,
the PWB was detected at relatively low incidence in branch bark, trunk bark and roots and the incidence did not change among
seasons. A survey of PWB in 27 fields in the Tohoku district of Japan showed that malformed flower buds were observed in paulownia
trees in almost all of the fields. PWB-phytoplasma was also detected by PCR from paulownia trees in almost all of the fields
in Iwate and Fukushima Prefectures. The frequencies of trees in which phytoplasma was detected by PCR were higher than those
in which symptoms were observed. These results indicated that PCR amplification of a PWB-specific DNA fragment is an effective
tool for practical diagnose and that PWB is widely distributed in the Tohoku district of Japan. 相似文献
57.
J. Plavec Ž. Budinšćak I. Križanac D. Škorić X. Foissac M. Šeruga Musić 《Plant pathology》2019,68(1):18-30
Flavescence dorée phytoplasma (FDp) is a quarantine pathogen associated with a severe and epidemic grapevine yellows disease representing a great threat for grapevine cultivation in Europe. An increase in disease spread prompted efforts to identify FDp strains in Croatia. Over 800 samples of grapevine together with presumed reservoir plants and almost 400 samples of Scaphoideus titanus and other potential vectors were collected countrywide and analysed. FDp isolates were characterized by multilocus sequence typing (MLST) of map, secY and uvrB-degV genes in order to determine genetic diversity and structure of FDp populations, and to trace transmission pathways. FD-related phytoplasmas were found in Croatia for the first time in alder, the invasive tree species Ailanthus altissima and leafhopper Phlogotettix cyclops. Phylogenetic analysis revealed the presence of three mapFD strain clusters: mapFD1, mapFD2 and mapFD3, and for the first time in Croatia a case of Palatinate grapevine yellows strain A (PGY-A). In total, 7 different map, 10 secY and 11 uvrB-degV genotypes were detected. The identification of 15 comprehensive FDp genotypes based on MLST suggests separate routes for disease introduction and propagation origins in Croatia. Moreover, high genetic variability of Croatian isolates indicates a complex ecological cycle of FDp involving various hosts. 相似文献
58.
59.
广东枣疯病植原体的鉴定 总被引:1,自引:1,他引:0
Several jujube plants with witches′ broom, little leaf, and big bud symptoms, which were likely infected by jujube witches′ broom (JWB) phytoplasma, were collected in Guangzhou, Guangdong Province. To identify the pathogen, PCR was performed using phytoplasma 16S rDNA universal primer pairs R16mF2/R1 and P1/P7 and SecA gene primer pair SecAfor1/rev3 with total DNA of the symptomatic plants as templates. Specific fragments, 1.4 kb, 1.8 kb, and 0.8 kb in length, were amplified from one of three symptomatic samples. Phylogenetic analysis based on 16S rDNA verified that the pathogen harming jujube plants in Guangzhou was jujube witches′ broom phytoplasma which belonged to 16SrV-B subgroup. Comparison results also showed that the 16S rDNA sequence of Guangzhou JWB phytoplasma shared the highest nucleotide identity (100%) with the reported jujube witches′ broom phytoplasma Japanese strain (AB442218) and JWB strain (AY197661) and shared the nucleotide identity ranging from 99.74% to 99.80% with the other JWB phytoplasma strains. In addition, phylogenetic analysis based on SecA also showed that Guangzhou jujube witches′ broom phytoplasma belonged to 16SrV-B subgroup and shared 99.28%-99.76% similarity with other phytoplasma strains. All these results suggested that jujube witches′ broom phytoplasma has infected jujube plants in Guangdong Province. 相似文献
60.
【目的】揭示小麦蓝矮植原体(WBD phytoplasma)的致病机理及其与寄主的互作关系,为植原体病害防治提供理论依据。【方法】以SWP16为诱饵,将SWP16构建到诱饵载体pBT3-STE和pBT3-SUC上,在小麦酵母双杂交膜系统cDNA文库中筛选与SWP16互作的蛋白,并确定最佳的筛库条件。通过α-半乳糖苷酶活性检测试验对筛选到的互作蛋白进行二次验证,并运用Uniprot对其进行Gene Ontology(GO)注释分析。【结果】以pBT3STE-SWP16作为筛库的诱饵蛋白,确定20 mmol/L 3-AT为筛选文库的最佳浓度,从小麦cDNA文库中筛选到20种互作蛋白,包括Rieske 蛋白、细胞色素b5、CASP蛋白等。经过复筛和α-半乳糖苷酶活性检测试验进一步验证了互作关系,其中14种互作蛋白参与运输、pH调节、光合作用、内质网应激反应、蛋白质泛素化等生理过程;18种互作蛋白存在于线粒体、内质网、细胞膜、细胞质、叶绿体和细胞壁等6种细胞组分中;20种互作蛋白的分子功能主要包括几丁质酶活性、反转运蛋白活性、脱水酶活性、转移酶活性和转录因子活性等。【结论】筛选获得20种与小麦蓝矮植原体效应蛋白SWP16互作的蛋白,有助于推动小麦蓝矮植原体与寄主互作关系的研究。 相似文献