梭梭幼苗根系分泌物提取方法的研究

为了探讨梭梭根系分泌物中的化学成分,本文采用水浸提取和循环灌溉两种方法对梭梭幼苗根系分泌物进行收集和提取,结合气相色谱-质谱联用分析技术,分析梭梭幼苗根系分泌物中组分和含量的差异。实验结果表明:循环灌溉收集的梭梭幼苗根系分泌物中检测到35种化合物,包括酚醌类、醛酮类、醇类、酸类、脂类和胺类化合物;硅烷化处理后,主要增加部分强极性有机酸,如苯甲酸。水浸提取梭梭幼苗根系分泌物的中性相组分共检测出27种化合物,但未检测到酚醌类化合物;碱性相组分共检测出23种化合物,醛酮类化合物种类减少,烷烃类化合物种类增加;酸性相组分中仅检测到10种化合物,以醇类化合物为主,占检出化合物总量的72.62%。收集梭梭幼苗根系分泌物循环灌溉是一种有效的方法。     

Carbon and nitrogen stocks and their mineralization potentials are higher under organic than conventional farming practices in Japanese Andosols

ABSTRACT

This study aimed at understanding whether and how long-term organic rice farming affects soil carbon (C) and nitrogen (N) stocks and their mineralization potentials in submerged rice paddies in Tochigi, Japan. An incubation experiment was carried out to assess the impacts of internal nutrient cycling after organic farming (OF) for 4–5 years (4OF), 8–9 years (8OF), and 12 years (12OF), compared with a conventional rice field (CF). Soil samples were collected at 0–15 cm and 15–20 cm in flooded rice fields after harvest in October 2013. pH and bulk density at 0–15 cm were significantly lower in 12OF fields than in CF fields (by 0.22 unit pH and 17.5%, respectively). Compared with CF, 12OF fields showed significant differences in soil organic carbon (SOC) and total nitrogen (TN), but 4OF and 8OF fields did not. In 8OF fields, the C decomposition (Co) and N mineralization (No) potentials were significantly higher (by 34.0% and 35.6%, respectively, at 0–15 cm, and by 67.1% and 24.5% at 15–20 cm) than in CF fields. Similarly, in 8OF fields at 0–15 cm, the Co:SOC and No:TN ratios were 19.8% and 23.2% higher, respectively, than in CF fields. Co, No, Co:SOC, and No:TN in 12OF fields were higher than those in CF fields, demonstrating the effects of prolonged organic rice farming. Additionally, in 12OF fields, C and N stocks were significantly higher (by 15.5% and 17.2%, respectively, at 0–15 cm, and by 4.8% and 12.1% at 15–20 cm) than in CF fields. Our findings suggest that long-term organic rice farming increases soil C and N stocks as well as C and N mineralization in Japanese Andosols.     

Stable carbon isotope ratios of water-extractable organic carbon affected by application of rice straw and rice straw compost during a long-term rice experiment in Yamagata,Japan

ABSTRACT

Hot-water- and water-extractable organic matter were obtained from soil samples collected from a rice paddy 31 years after the start of a long-term rice experiment in Yamagata, Japan. Specifically, hot-water-extractable organic carbon and nitrogen (HWEOC and HWEON) were obtained by extraction at 80°C for 16 h, and water-extractable organic carbon and nitrogen (WEOC and WEON) were obtained by extraction at room temperature. The soil samples were collected from surface (0–15 cm) and subsurface (15–25 cm) layers of five plots that had been treated with inorganic fertilizers alone or with inorganic fertilizers plus organic matter, as follows: PK, NPK, NPK plus rice straw (RS), NPK plus rice straw compost (CM1), and NPK plus a high dose of rice straw compost (CM3). The soil/water ratio was 1:10 for both extraction temperatures. We found that the organic carbon and total nitrogen contents of the bulk soils were highly correlated with the extractable organic carbon and nitrogen contents regardless of extraction temperature, and the extractable organic carbon and nitrogen contents were higher in the plots that were treated with inorganic fertilizers plus organic matter than in the PK and NPK plots. The HWEOC and WEOC δ¹³C values ranged from ?28.2% to ?26.4% and were similar to the values for the applied rice straw and rice straw compost. There were no correlations between the HWEOC or WEOC δ¹³C values and the amounts of HWEOC or WEOC. The δ¹³C values of the bulk soils ranged from ?25.7% to ?23.2% and were lower for the RS and CM plots than for the PK and NPK plots. These results indicate that HWEOC and WEOC originated mainly from rice plants and the applied organic matter rather than from the indigenous soil organic matter. The significant positive correlations between the amounts of HWEOC and HWEON and the amount of available nitrogen (P < 0.001) imply that extractable organic matter can be used as an index for soil fertility in this long-term experiment. We concluded that the applied organic matter decomposed more rapidly than the indigenous soil organic matter and affected WEOC δ¹³C values and amounts.     

Changes in forest soil organic matter pools after a decade of elevated CO2 and O3

The impact of rising atmospheric carbon dioxide (CO₂) may be mitigated, in part, by enhanced rates of net primary production and greater C storage in plant biomass and soil organic matter (SOM). However, C sequestration in forest soils may be offset by other environmental changes such as increasing tropospheric ozone (O₃) or vary based on species-specific growth responses to elevated CO₂. To understand how projected increases in atmospheric CO₂ and O₃ alter SOM formation, we used physical fractionation to characterize soil C and N at the Rhinelander Free Air CO₂-O₃ Enrichment (FACE) experiment. Tracer amounts of ¹⁵NH₄⁺ were applied to the forest floor of Populus tremuloides, P. tremuloides-Betula papyrifera and P. tremuloides-Acer saccharum communities exposed to factorial CO₂ and O₃ treatments. The ¹⁵N tracer and strongly depleted ¹³C-CO₂ were traced into SOM fractions over four years. Over time, C and N increased in coarse particulate organic matter (cPOM) and decreased in mineral-associated organic matter (MAOM) under elevated CO₂ relative to ambient CO₂. As main effects, neither CO₂ nor O₃ significantly altered ¹⁵N recovery in SOM. Elevated CO₂ significantly increased new C in all SOM fractions, and significantly decreased old C in fine POM (fPOM) and MAOM over the duration of our study. Overall, our observations indicate that elevated CO₂ has altered SOM cycling at this site to favor C and N accumulation in less stable pools, with more rapid turnover. Elevated O₃ had the opposite effect, significantly reducing cPOM N by 15% and significantly increasing the C:N ratio by 7%. Our results demonstrate that CO₂ can enhance SOM turnover, potentially limiting long-term C sequestration in terrestrial ecosystems; plant community composition is an important determinant of the magnitude of this response.     

Compartmentalization of the soil animal food web as indicated by dual analysis of stable isotope ratios (N/N and C/C)

The soil animal food web has become a focus of recent ecological research but trophic relationships still remain enigmatic for many taxa. Analysis of stable isotope ratios of N and C provides a powerful tool for disentangling food web structure. In this study, animals, roots, soil and litter material from a temperate deciduous forest were analysed. The combined measurement of δ¹⁵N and δ¹³C provided insights into the compartmentalization of the soil animal food web. Leaf litter feeders were separated from animals relying mainly on recent belowground carbon resources and from animals feeding on older carbon. The trophic pathway of leaf litter-feeding species appears to be a dead end, presumably because leaf litter feeders (mainly diplopods and oribatid mites) are unavailable to predators due to large size and/or strong sclerotization. Endogeic earthworms that rely on older carbon also appear to exist in predator-free space. The data suggest that the largest trophic compartment constitutes of ectomycorrhizal feeders and their predators. Additionally, there is a smaller trophic compartment consisting of predators likely feeding on enchytraeids and potentially nematodes.     

FACE对三系杂交籼稻汕优63根系活性影响的研究

2005、2006年利用我国惟一的农田开放式空气CO2浓度增高（FACE）研究平台,设计施N量为125kg·hm^-2（LN）、250kg·hm^-2（NN）处理,研究大气CO2浓度比对照高200umol·mol^-1的FACE处理对三系杂交籼稻汕优63根系活性的影响。结果表明：（1）FACE处理使汕优63不同生育时期单位干质量根系的总吸收面积、活跃吸收面积、α-萘胺氧化量等根系活性指标均极显著小于对照。由于FACE处理促进汕优63根系发生量的大幅度增加,因此分蘖期、拔节期其单穴根系活性与对照多无明显差异,到抽穗期FACE处理单穴根系活性显著大于对照;（2）拔节期、抽穗期汕优63每穴的不定根数、不定根总长度、根系体积、根干质量与单位干质量根系活性的关系密切,根量越大单位于质量根系活性越低;（3）不同生育时期汕优63植株含氮率与单位干质量的根系活性多呈正相关,植株碳氮比与单位干质量的根系活性多呈负相关;（4）FACE处理汕优63根系生长量大、植株含氮率低、碳氮比高等可能是造成其单位干质量根系活性低于对照的重要原因。     

农药废水对秀丽隐杆线虫毒性效应及其主要有毒组分

应用battery生物测试法,检测研究农药废水对秀丽隐杆线虫的生命周期、繁殖速率、生殖能力、头摆和身体弯曲频率等影响的生物毒性,及其引发生物毒性的主要有机污染物。结果表明,在已经达到国家废水排放标准[GB 8978—1996]情况下,处理出水对受试动物仍然存在致毒效应;线虫的世代周期对进水的毒性最为敏感,产卵数量对出水的毒性最为敏感;进水毒性主要来自易受酸性调节影响的有机污染物,出水毒性主要来自易受碱性调节影响的有机污染物。结果表明,该项生物测试的毒性参数,可用于指示存在于低CODCr废水中的生物毒性;所用的毒性鉴别评价（TIE）方法,可用于鉴别废水中引发致毒效应的关键污染物。废水中悬浮颗粒污染物的生物毒性至关重要,尚未研究,有待继续。     

中国农田表土有机碳含量变化特征——基于国家耕地土壤监测数据

通过对299个国家级耕地土壤监测点20余年数据的统计分析,评价了我国农田表土有机碳含量变化情况和固碳潜力。结果表明,全国约80％试验点有机碳年平均相对增长率（Average relative annual increment,ARAI）在-1．5％～7．5％。中国农田表土有机碳含量整体呈上升趋势。东北、华北等6个地理区域分析得出,华北、华东、西南农田表土有机碳含量显著增加;华东地区有机碳增加的农田面积占全国农田比例最大,东北最小。旱地和水田有机碳含量增加显著;水田有机碳增加的试验点所占比例大于旱地;对ARAI与初始有机碳含量进行相关分析得出,我国旱地和水田有机碳潜在储存能力估计值分别为17．2和27．7g·kg^-1。农田土壤类型中水稻土和褐土有机碳含量增加显著;黑土有机碳含量下降样本所占比例最高。对我国各典型种植制度分析得出,双季稻、麦-稻、麦-玉、单季小麦种植制度下农田有机碳有了显著增加;麦玉轮作较其他种植制度的农田有机碳年平均相对增长率高。     

红壤丘陵双季稻稻田农田生态系统不同施肥下碳汇效应及收益评估

收集江西一个红壤水稻田长期肥料试验中产量、施肥量、土壤有机碳等资料,结合对农田生产中各项生产资料和管理活动的投入进行调查,对不同施肥处理下系统的生产力、碳投入排放与碳收集效应进行分析,并估算系统的碳汇。评价了不同处理下生产力的碳成本与碳汇效益。结果表明,种植双季稻下化肥配施和化肥有机肥配施下的稻田总碳汇分别为-2．2和-3．2t C·hm^-2·a^-1,但在两季生产中并无显著差异;相应地,有机无机肥配施比纯化肥配方施肥产量提高了30％左右,而碳汇量提高了50％左右。证明有机无机配合施肥是一种可以增产增汇的关键农业生产管理途径。     

荒漠草原区固沙人工柠条林生长过程中土壤性质演变规律

开展荒漠草原区固沙人工林生长过程中土壤性质研究,对于沙化草地土壤质量演变规律分析和人工林有效管理均具有重要的指导价值。通过野外调查取样和室内分析,测定6,15,24,36年柠条林地土壤机械组成、土壤养分、pH和电导率以及土壤水分与温度等指标变化,分析荒漠草原区种植固沙柠条人工林对土壤性质的影响。结果表明,从6年到15年柠条林地,土壤粗沙粒和细沙粒成分显著升高(P<0.05),但从24年柠条林地开始土壤粗沙粒和细沙粒含量显著下降(P<0.05),土壤极细沙粒和粘粉粒含量开始显著增加(P<0.05)。从24年柠条林地开始,土壤有机碳、全N和全P含量显著升高,土壤电导率和水分含量呈现增加趋势,而土壤pH和温度显著下降;而且土壤全N积累量高于土壤有机碳,土壤C/N随着柠条林龄的增加呈逐渐下降趋势。在荒漠草原区,人工柠条林发育生长有利于土壤肥力的提高和土壤环境的改善,而且需要经历一个较长的发育过程。