武定鸡红细胞免疫功能研究及其意义

应用红细胞Ｃ３ｂ受体花环（Ｃ３ｂＲＲ）和免疫复合物花环（ＩＣＲ）试验,证实武定鸡红细胞表面存在补体Ｃ３ｂ受体（Ｃ３ｂｒｃｃｃｐｔｏｒ,Ｃ３ｂＲ）,表明红细胞免疫系统（Ｒｃｄｃｃｌｉｍｍｕｎｅｓｙｓｔｅｍ,ＲＣＩＳ）的概念亦适用于这种动物。武定鸡的ＲＣＩＳ有其独特之处,文中对武定鸡红细胞Ｃ３ｂＲＲ和ＩＣＲ分别代表红细胞表面Ｃ３ｂ“空位”和“占位”状态的含义做出了新的解释。     

基于C2C网站的农产品网络营销实现

本文首先介绍了网络营销的含义,并说明采用网络营销的方法推销农产品的意义,然后对当前重要的网络营销网站淘宝网、易趣网及拍拍网上农产品的销售情况进行说明,最后说明采用C2C网站进行网络营销时如何解决安全问题.     

口蹄疫病毒非结构蛋白2C基因在昆虫细胞中的表达及应用

研究口蹄疫病毒(FMDV)非结构蛋白(NSP) 2C在区分灭活疫苗免疫动物与自然感染动物方面的意义.本研究将FMDV NSP 2C基因,克隆到穿梭载体pFast-bac- HT-B,将其转入含骨架载体Bacmid的DH10Bac,经蓝白斑筛选得到重组骨架质粒Bacmid-2C.将Bacmid-2C转染昆虫细胞Sf9,鉴定正确后,经3次增殖获得高滴度的P-3代病毒后,在High Five细胞中进行目的蛋白的表达.SDS-PAGE结果显示在High Five细胞中得到了相对分子质量约为38.93 ku的目的蛋白2C,Western blotting及Dot-ELISA结果显示,该表达产物对FMDV感染动物阳性血清有良好的反应性.以电泳纯化的2C蛋白为抗原建立间接ELISA,检测健康非免疫动物、免疫动物及FM-DV试验感染动物血清,结果表明2C-ELISA不但能区分免疫动物和感染动物的血清,而且还能检测FMDV感染早期动物血清中的2C抗体.说明昆虫细胞表达的2C蛋白可作为FMDV疫苗免疫动物与自然感染动物鉴别诊断的良好抗原.2C基因在Bac-to-Bac系统中的成功表达为建立通过检测几种NSP抗体,筛查感染及隐性带毒动物,净化畜群的方法奠定了基础.     

Establishment of Detection Methods for Dexamethasone Sodium Phosphate in Injections by UPLC-PDA

A detection method of dexamethasone sodium phosphate in houttuynia injection,bupleurum injection,vitamin C injection,lincomycin hydrochloride injection,and asragulus polysacharin injection was established by UPLC-PDA.The Waters ACQUITY UPLC^® BEH C18 column(2.1 mm×50 mm,1.7 μm) was used,the mobile phase consisted of 0.75% triethylamine phosphatic liquor/solution (pH 3.0)-methyl alcohol-acetonitrile(50:45:5),the flow rate was 0.3 mL/min,the column temperature had remained 25℃,photodiode array detector wavelength range was 190 to 400 nm,the recorded wavelength was 242 nm,the injection volume was 10 μL.The linear range of dexamethasone sodium phosphatel was 10.0 to 200.0 mg/L (R²=0.9999),the mean recovery and RSD for dexamethasone sodium phosphate in houttuynia injection,bupleurum injection,vitamin C injection,lincomycin hydrochloride injection and asragulus polysacharin injection were 97.54% and 1.14%,101.59% and 0.19%,100.92% and 0.92%,99.82% and 0.73%,100.08% and 0.62%,respectively.This method was accurate,easy and fast,it was suitable for detection of dexamethasone sodium phosphatein houttuynia injection and other four kinds of injections.     

Bovine Cells Infected in Vivo with Theileria Annulata Express CD11b,the C3bi Complement Receptor

Forsyth, L.M.G., Jackson, L.A., Wilkie, G., Sanderson, A., Brown, C.G.D. and Preston, P.M., 1997. Bovine cells infected in vivo with Theileria annulata express CD11b, the C3bi complement receptor. Veterinary Research Communications, 21 (4), 249-263Bovine cells from cattle infected with Theileria annulata were phenotyped with monoclonal antibodies recognizing bovine leukocyte antigens. Macroschizont-infected, transformed cell lines prepared from peripheral blood mononuclear cells of cattle, infected with sporozoites, were assessed by flow cytometry; parasitized cells in tissues from infected cattle were examined by immunocytochemical techniques. Co-expression of markers for different cell lineages by the cell lines precluded a definite conclusion as to their phenotypic origins. For, while the pattern of leukocyte antigens expressed by these in vivo-derived schizont-infected cells, which included CD11b, was indicative of a myeloid origin, the possibility that they were NK cells could not be excluded. The monoclonal antibody (MAb) IL-A15, which recognizes CD11b, reacted with a high proportion of parasitized cells in sections of tissues from infected cattle at all stages of acute disease. Mononuclear cells infected with parasites at all stages of differentiation, from macroschizont to microschizont, expressed CD11b. Such parasitized cells occurred throughout the lymphoid tissues, being found in the thymus, spleen and lymph nodes, particularly the prescapular node draining the site of infection, the hepatic, mesenteric and precrural nodes, as well as in the reticulo-endothelial tissue of the liver, kidney, lung, abomasum, adrenal and pituitary glands. These observations provided the first evidence for a myeloid origin for the parasitized T. annulata cells found in infected bovine tissues and blood and suggested a mechanism whereby schizonts could transfer from cell to cell during mechanical infection with schizont-infected cells.     

中苜一号紫花苜蓿碳同化分配与转移速率的研究

对紫花苜蓿(Medicago sativa L.)(中苜一号)的生长条件进行控制,并在分枝期对其进行¹³C脉冲标记,收集标记后第6,12,18,24,30 d的叶、茎、根以及土壤样品,测定各样品中¹³C含量,以此来探讨苜蓿光合碳的吸收与转移。结果表明:苜蓿各组分光合产物的分配顺序为茎>叶>根;¹³C转移速率为叶>茎>根,随着时间的延长,苜蓿各组分¹³C含量逐渐减少,最终达到稳定状态;渗漏到土壤中的¹³C含量在0~10 cm土壤层显著高于10~20 cm,但10~20 cm的¹³C含量比较稳定。     

我国西藏四种植物染色体数目观察

本文对西藏四种植物进行染色体观察，这四种植物的染色体数目均为国内外首次报道。     

细菌鞭毛蛋白作为佐剂的研究进展

细菌鞭毛蛋白作为Toll样受体5(TLR5)或NOD样受体C4(NLRC4)的配体,其结构决定了其既有抗原性又具有佐剂效应。将细菌鞭毛蛋白与外源抗原混合或融合表达,已获得多种有效的候选疫苗。细菌鞭毛蛋白佐剂效应主要是通过TLR5和NLRC4信号途径协同实现的。TLR5位于细胞表面,可触发炎性因子、趋化因子和Ⅰ型干扰素的分泌,启动天然免疫应答。NLRC4是细胞质中的模式识别受体,可识别细胞质中的多种配体并诱导相应免疫应答。另外,细菌鞭毛蛋白能募集T淋巴细胞和B淋巴细胞至次级淋巴器官,促进DCs和T淋巴细胞的活化。细菌鞭毛蛋白的可塑性将会使得以细菌鞭毛蛋白为基础的疫苗成为当前和未来研究的热点。     

马蹄金与匍茎翦股颖光合生理生态的比较研究

研究表明，１．通过光饱和点、光补偿点、ＣＯ＿２补偿点及叶绿素ａ／ｂ比值等测定，马蹄金与匍茎剪股颖均属喜光、耐荫的Ｃ＿３植物。马蹄金对强光适应性大于匍茎翦股颖。因而在芜湖地区作为大田草坪种植，则马蹄金优于匍茎葛股颖。２．ＮａＨＳＯ＿３均有增强光合速率、加速光合产物输出、增加于物质积累及提高生物产量的效应。光呼吸抑制剂在草坪生产上大有可为。     

维生素C对热应激状态下肉仔鸡生产性能及维生素C合成能力的影响

选用21日龄AA肉仔鸡240只,研究了热应激（室温32－35℃,相对湿度80％－825）和非应激（室温20－25℃,相对湿度65％－70％）状态下日粮添加维生素C对肉仔鸡生产性能和体内维生素C合成能力的影响。试验结果表明,肉仔鸡在应激状态下血浆维生素C含量降低,日粮补充200mg/kg维生素C可以明显提高血浆维生素C含量。热应激导致肉仔鸡肝脏维生素C合成关键酶－古洛糖酸内酯氧化酶活性明显降低,使维生素C含量。热应激导致肉仔鸡肝脏维生素C合成关键酶－古洛糖酸内酯氧化酶活性明显降低,使维生素C的合成能力减弱,日粮汪厍维生素C可提高应激状态下肉仔鸡肝脏古洛糖酸内酯氧化酶活性,随肉仔鸡日龄的增加,肉仔鸡肝脏古洛糖酸内酯氧化酶活性有上升趋势,表明维生素C合成能力随日龄的增加逐渐提高。