电感耦合等离子体质谱法测定水产品中砷含量的不确定度评定

采用电感耦合等离子体质谱法测定了FAPAS质控样品T07279QC中砷含量,对其测定方法的不确定度进行了评估,分析了不确定度的主要来源,并计算了各不确定度分量,结果表明,不确定度主要来源于标准曲线拟合、测量结果的重复性、方法回收率、标准溶液配制,当砷含量为16.43mg/kg时,扩展不确定度为0.70mg/kg(k=2)。该方法适用于以线性回归得出检测结果的不确定度评定。     

From monsoons to mantas: seasonal distribution of Manta alfredi in the Maldives

The Republic of Maldives in the central Indian Ocean is home to large numbers of manta rays, Manta alfredi. They are known to undertake seasonal migrations within the Maldives, but these movements have not been well documented. The aims of this study were to map the seasonal distribution of manta rays within the Maldives, and to provide some indications of the physical and biological oceanographic processes affecting their distribution. The seasonal distribution of mantas was determined from a national survey of fishermen, interviews with experienced divers and personal observations. The data demonstrate that the distribution of mantas is strongly influenced by the seasonally reversing monsoon currents. Mantas occur on the downstream sides of the atolls, and are rare on the upstream sides, switching sides biannually as the monsoon currents change direction. These seasonally alternating currents are driven by monsoon winds which also alternate according to the season, and bring clear oceanic water to the upstream sides of the atolls. As the currents pass over the Maldives ridge, nutrient‐rich waters are lifted to the surface, promoting phytoplankton blooms (as demonstrated by the distribution of chlorophyll‐a) on the downstream sides of the atolls. This manifestation of the island mass effect supports an abundance of zooplankton, which in turn supports the manta rays.     

海藻中铝形态分析技术

建立了高效液相色谱-电感耦合等离子体质谱联用技术（HPLC-ICP-MS）分析海藻及其制品中三价铝的方法,用提取液0.040 mol/L氢氧化锂＋0.006 mol/L吡啶-2,6-二羧酸＋-0.090 mol/L冰醋酸在70℃恒温水浴摇床中浸提2h,阳离子交换色谱柱分离,ICP-MS检测.样品加标量在5.0 mg/kg和50.0 mg/kg时的平均回收率在90.8％以上,相对标准偏差均＜4％（n=5）.本研究证明,该方法测定海藻及其制品中的三价铝较为准确、可靠,可为制定农业行业标准提供技术支撑.     

Antibody screening identifies 78 putative host proteins involved in Cyprinid herpesvirus 3 infection or propagation in common carp,Cyprinus carpio L

Cyprinid herpesvirus 3 (CyHV‐3) is the aetiological agent of a serious and notifiable disease afflicting common and koi carp, Cyprinus carpio L., termed koi herpesvirus disease (KHVD). Significant progress has been achieved in the last 15 years, since the initial reports surfaced from Germany, USA and Israel of the CyHV‐3 virus, in terms of pathology and detection. However, relatively few studies have been carried out in understanding viral replication and propagation. Antibody‐based affinity has been used for detection of CyHV‐3 in enzyme‐linked immunosorbent assay and PCR‐based techniques, and immunohistological assays have been used to describe a CyHV‐3 membrane protein, termed ORF81. In this study, monoclonal antibodies linked to N‐hydroxysuccinimide (NHS)‐activated spin columns were used to purify CyHV‐3 and host proteins from tissue samples originating in either CyHV‐3 symptomatic or asymptomatic fish. The samples were next analysed either by polyacrylamide gel electrophoresis (PAGE) and subsequently by electrospray ionization coupled to mass spectrometry (ESI‐MS) or by ESI‐MS analysis directly after purification. A total of 78 host proteins and five CyHV‐3 proteins were identified in the two analyses. These data can be used to develop novel control methods for CyHV‐3, based on pathways or proteins identified in this study.     

泥鳅混合选育F_2代生长性能分析

以泥鳅太湖群体为基础群体,采用混合选育方法进行了人工选育,对产生的混合选育F2代进行生长性能分析。结果表明：泥鳅混合选育F2代的日增重率和日增长率分别达到0.022g/d和0.036cm/d,比对照组提高了4.8%和2.9%。混合选育群体体重变异系数和体长变异系数分别比对照群体降低了9.8%和8.1%,经过一代人工选育,泥鳅生长性能进一步提高,且群体规格趋于整齐。     

成熟番茄的图像识别及其位姿的获取研究

针对自然生长状态下成熟番茄图像的识别问题,提出选用YIQ颜色空间,采用Otsu最大类间方差法分割图像,然后用面积阈值的方法消除噪声,来获取成熟番茄图像的目标区域;然后用矩法来求目标区域的质心位置和惯性主轴,用惯性主轴的角度来标识果实果轴的方向,进而获取果实的生长姿态。实验结果表明,用这种方法可以取得较好的效果。     

外源乙烯对不同品种辣椒种子萌发的影响

以一年生栽培辣椒(Capsicum annuum)的4个变种为材料,使用不同质量浓度的乙烯(0~250 mg/L)处理辣椒种子,研究乙烯对辣椒种子萌发的影响.结果表明:乙烯对辣椒种子的发芽势和发芽率没有显著影响;低质量浓度乙烯(50 mg/L)对发芽指数没有显著影响,而高质量浓度乙烯(100~250 mg/L)显著抑制发芽指数;辣椒种子下胚轴的长度、弯曲度和胚根的长度均被乙烯显著抑制,乙烯质量浓度越高,抑制作用越强;下胚轴的粗度随着乙烯质量浓度的提高,表现为先增加后降低的趋势,但是最终粗度仍大于对照.因此,可得出结论,乙烯对辣椒种子萌发的影响随乙烯质量浓度的不同而有所不同.     

大批量数据自动移植方案的设计与开发——在不同版本、不同编码格式的Oracle数据库之间

通过在同一进程中灵活变换系统变量NLS_LANG的值,融合数据导入导出技术,从而实现大批量数据的在不同版本、不同编码格式的Oracle数据库之间的自动移植.     

长吻鮠形态性状对体质量的影响

为探究长吻鮠(Leiocassis longirostris)形态性状对体质量的影响,随机选取115尾14月龄的长吻鮠,测定其体质量(Y)、全长(X1)、体长(X2)、体高(X3)、体宽(X4)、头长(X5)、头高(X6)、头宽(X7)、吻长(X8)、尾柄长(X9)、尾柄高(X10)和躯干长(X11)共12个性状,进行相关分析、通径分析和多元回归分析。结果显示,11个形态性状与体质量的相关性均达到极显著水平(P<0.01);全长、头宽、体长、吻长和尾柄高5个性状的通径系数达到显著水平(P<0.05),其中,全长对体质量的直接作用最大;决定系数分析结果与通径分析结果一致,全长、头宽、尾柄高、体长和吻长的共同决定系数总和为0.911,说明这5个性状是影响长吻鮠体质量的主要因素;运用多元回归分析建立以体质量(Y)为因变量,全长(X1)、体长(X2)、头宽(X7)、吻长(X8)和尾柄高(X10)为自变量的线性回归方程：Y=–301.368+5.888X1+5.988X2+21.482X7+16.408X8+34.878X10。本研究可为长吻鮠生长相关性状选育提供理论依据,在长吻鮠优良个体选育中具有重要意义。     

水产品中氯霉素、甲砜霉素和氟甲砜霉素残留量高效液相色谱-串联质谱内标测定方法的研究

建立了水产品中3种氯霉素类抗生素残留的高效液相色谱-串联质谱(HPLC-MS/MS )测定法.以氘代氯霉素(d5-CAP)为内标,样品在碱性条件下用乙酸乙酯提取,提取液氮气吹干,正己烷液液分配脱脂后,采用配有ESI源的LC-MS/MS选择反应监测(SRM)负离子模式测定,可同时对水产品中的氯霉素、甲砜霉素和氟甲砜霉素进行定性和定量测定.方法简化了样品前处理过程,省去固相萃取步骤,具有操作简便、有机试剂消耗量少、测定周期短和定量更准确等优点.方法的检出限:氯霉素为0.01 μg/kg,甲砜霉素和氟甲砜霉素为0.03 μg/kg.