Canine retinal surgery

Retinal detachment is common in dogs and presents major challenges for the veterinary ophthalmologist. This article reviews recent advances in both technology and technique that have greatly increased the success rate of retinal reattachment surgery. Internal and external techniques for the repair of retinal detachments are discussed, as is the etiopathogenesis of vitreoretinal disease in the dog.     

长穗偃麦草与中间偃麦草杂种成熟胚离体培养与再生体系的建立

以长穗偃麦草(Elytrigia elongata)和中间偃麦草杂交种(Elytrigia hybrid;E. elongata×E. intermedia)的成熟胚为外植体,进行组培再生体系的研究。结果表明:诱导愈伤组织的适宜培养基为MS+CH(500 mg/L)+2,4-D(1.0 mg/L)+6-BA(0.2 mg/L),其诱导率为73%,适宜分化培养基为MS+6-BA(4.0 mg/L)+NAA(0.5 mg/L),分化率最高,为37.8%。本研究建立了一套有效的以成熟胚为外植体偃麦草杂种组织培养再生体系。     

滇丁香非试管智能化快繁技术研究

滇丁香是一种具有较高开发价值的园林观赏树种,利用植物非试管智能化快繁技术进行其扦插苗的规模化快繁,具有生根快、生根率高、移栽成活率好的优势。试验结果证明,滇丁香扩繁最佳的扦插方法是采用其半木质化枝条作为插条,用200 mg/kg的ABT2处理2 h后扦插;其次是用200 mg/kg的NAA或国光生根处理2 h后扦插。在规模化生产中,采用国光生根2 000 mg/kg快速沾根2~5 m in的方法,也可达到生根率90%左右的效果;同时电磁场处理苗床具有杀菌及提高生根率的作用。用非试管扦插快繁方法将未生根植株进行回插,1个月后仍可获得90%以上的生根效果,该特点与其他树种扦插苗的表现显著不同,有利于提高滇丁香扦插苗的生产效率。     

Histopathological and immunohistochemical features of proliferative lesions in the pituitary pars distalis of rats

Pituitary proliferative lesions derived from the endocrine cells of the pars distalis are frequently encountered and adenomas/carcinomas are a common cause of death in standard 2-year carcinogenicity studies using various rat strains, especially Sprague-Dawley. This report describes the immunohistochemical characteristics of pituitary tumors derived from the pars distalis in rats. Prolactin (PRL)-containing tumors are the most common, with PRL/growth hormone (GH) dual positive tumor masses (PRL/GH co-positive tumor masses) being more prevalent than only PRL-positive tumor masses (PRL single-positive tumor masses). GH-containing tumors are relatively numerous and many of these are also PRL/GH co-positive tumor masses. TSH-containing tumors are common in females. PRL-containing tumors have been shown to increase the incidence of hyperlactation in males and mammary adenomas/adenocarcinomas in females, suggesting that these masses are functional tumors.     

中麻黄新疆产地生态气候适宜性分析

以实地调查采样点、标本查阅和文献研究为基础,结合《中药材产地适宜性分析地理信息系统-I》进行综合分析中麻黄在新疆的适宜生长区域,为中麻黄规范化种植、科学选址及合理规划生产布局提供依据。结果表明,中麻黄全疆广为分布,适宜区为吐鲁番盆地一带及准噶尔盆地北部的库车、轮台、柯坪、阿图什、阿合奇及阿克陶县。     

不同保存方法对中麻黄基因组DNA提取效果的影响

通过对不同方法采集和保存的中麻黄样品基因组DNA提取效果进行比较,确定中麻黄的最佳采集和保存方法。应用改良CTAB法提取中麻黄基因组DNA,并采用琼脂糖凝胶电泳检测其完整性,利用紫外分光光度计法测定其浓度和纯度。结果表明,新鲜样品采集后立即冷冻保存或置于液氮和硅胶后冷冻保存,均能提取出高质量的基因组DNA;鲜样阴干几天后冷冻保存会导致基因组DNA降解;阴干后在室温下保存则降解更严重。采集的中麻黄鲜样采取不同方法带回实验室均不影响基因组DNA的提取效果,但应在低温环境中保存才能有效防止基因组DNA的降解。