比格犬脑垂体远侧部细胞的电镜观察

透射电镜观察表明,比格犬(Beagle)脑垂体远侧部实质中含有6种细胞,即生长激素细胞、催乳激素细胞、促甲状腺激素细胞、促性腺激素细胞、促肾上腺皮质激素细胞和滤泡—星形细胞,它们各有其超微结构特征。生长激素细胞呈圆形;胞核为圆形;分泌颗粒很多,圆形,直径约233～465nm,催乳激素细胞多呈不规则形;胞核为不规则形;分泌颗粒很多,圆形,直径约310～620nm。促甲状腺激素细胞呈圆形或卵圆形;胞核为圆形或卵圆形;分泌颗粒较少,直径约78～232nm。促肾上腺皮质激素细胞呈圆形或卵圆形;胞核为圆形或卵圆形;分泌颗粒较多,圆形,直径约155～310nm,分布于细胞周边。促性腺激素细胞呈圆形或椭圆形;胞核为圆形或椭圆形;分泌颗粒较少,呈圆形、卵圆形、杵状或不规则形,长径约155～434nm。滤泡—星形细胞呈星形或不规则形,有突起伸入相邻细胞之间;胞核为圆形或卵圆形;胞质内无分泌颗粒。     

中麻黄的适应性及其抗逆性栽培

中麻黄具有较强的旱生性和耐盐性 ,在逆境盐碱地栽培其经济效益、缓解天然麻黄资源破坏、减缓土地沙化的生态效益比较显著 ,是干旱区盐碱地农村调整种植结构、退耕还林的优良植物种。     

中间锦鸡儿冠层降雨再分配特征

以荒漠草原人工中间锦鸡儿(Caragana intermedia)灌丛为对象,研究降雨量及降雨强度对两类形态灌丛(健康组和退化组)穿透雨、树干茎流和冠层截留的影响。结果表明:①试验期间共观测到有效降雨33次,以中小降雨事件为主,总降雨量为251mm,次平均降雨量和平均降雨强度分别为7.6mm和1.14mm·h^-1,其中降雨量<2mm,降雨强度<1mm·h^-1的降雨事件出现次数最多。②健康组和退化组中间锦鸡儿平均穿透雨率、树干茎流率和冠层截留率分别为66.7%、8.5%、24.8%和75.4%、8.7%、16.0%,两类形态灌丛之间穿透雨和截留存在显著差异(P<0.05)。③两类形态灌丛的穿透雨量、树干茎流量、截留量与降雨量成线性函数、对数函数和幂函数关系,且其穿透雨率、树干茎流率和截留率与降雨量、降雨强度之间成对数函数和幂函数关系。灌丛形态退化显著改变了冠层降雨再分配格局,冠层截留显著降低,穿透雨显著增加,潜在提高了降水对土壤水分的直接补充,一定程度上有利于人工中间锦鸡儿稳定性的维持。     

Effects of cordycepin on motor and cognition in Parkinson disease mice induced by MPTP

AIM:To investigate the effects of cordycepin on the motor and cognition in Parkinson disease mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). METHODS:C57BL/6 mice were intraperitoneally injected with MPTP at a dose of 30 mg/kg daily for consecutive 8 d to establish the model of Parkinson disease. HE staining was used to observe the cell number in the substantia nigra pars compacta (SNpc) from the mice. Western blot was used to detect the protein level of tyrosine hydroxylase (TH) in substantia nigra (SN). The effects of cordycepin on the motor, emotional change and cognitive behavior of the Parkinson disease mice were examined by open-field test (OFT), sponta-neous alternating behavior (SAB) and water maze test (WMT), respectively. RESULTS:Cordycepin significantly reduced the apoptosis of cells in SNpc and reversed the decrease in the expression of TH in SN induced by MPTP (P<0.05). Furthermore, cordycepin was able to improve the average speed in OFT (P<0.05), and increased the total number of arm entry and the accuracy in SAB (P<0.05), but had no obvious effect on the latency in WMT. CONCLUSION:Cordycepin is capable of attenuating the impairments of motor and explorative ability in the early stage of Parkinson disease mice, but does not alter the cognitive dysfunction.     

比格犬脑垂体结节部细胞的电镜观察

透射电镜观察表明,比格犬(Beagle)脑垂体结节部实质中含有2类细胞,即含分泌颗粒的细胞和不含分泌颗粒的细胞．前者根据分泌颗粒的大小至少可以分为3种,其分泌颗粒直径分别约为310～605、155～388和124～202nm。     

干旱风沙区营造人工柠条林对退化沙地土壤水分运动参数的影响

以宁夏盐池沙区退化沙地为研究对象,探讨其在营造人工柠条灌木林后,对土壤水分运动参数的影响,研究结果显示:退化沙地营造人工柠条林后,0～40 cm土层的土壤水分扩散率呈现增加的趋势;0～40 cm土层的土壤基质势明显低于对照天然草地;0～40 cm土层的土壤比水容量随造林密度的增加测定值降低;0～40 cm土层的土壤比水容量林地明显低于对照天然恢复草地;人工柠条林土壤非饱和导水率显著低于对照天然草地,其大小排序为天然草地>2 490丛.hm-2柠条林>1 665丛.hm-2柠条林>3 330丛.hm-2柠条林。     

CiF3H基因克隆及功能分析

黄烷酮-3-羟化酶(F3H)是类黄酮合成途径的中枢位点,调控代谢途径中黄酮醇和花青素合成。文章根据转录组数据库中F3H基因中间片段设计特异引物,以中间锦鸡儿cDNA为模板,克隆得到CiF3H基因全长,通过序列分析、系统进化分析加以确认。qRT-PCR检测分析发现,中间锦鸡儿CiF3H基因受紫外、NaCl和ABA等胁迫诱导时表达模式具有相同规律。CiF3H基因在叶和根中表达量相当,茎中较少。异源表达CiF3H基因拟南芥的总黄酮含量少量增加,花青素含量降低。