河北省2018年第三季度马铃薯价格走势分析

依据对马铃薯价格的实时监控，以2018年河北省第三季度马铃薯价格为主要研究对象，阐明了2018年河北省马铃薯价格横向水平及纵向变化趋势，并对影响价格的因素进行了数据分析。结果显示：在河北、山东、甘肃3大产区及全国，2018年第三季度河北省马铃薯价格处于最低水平，明显低于全国平均水平，但年同比增幅最大。分析表明：马铃薯库存、气候的变化以及马铃薯收获期是影响第三季度河北省马铃薯价格的主要因素。最后，根据分析结果，提出调整种植结构、适时出售的建议。     

诱导Paracin1.7生成的刺激因子的分离与纯化

旨在分离纯化枯草芽孢杆菌(Bacillus subtilis)中能够诱导细菌素Paracin1.7分泌的刺激因子,并探明其对副干酪乳杆菌(Lactobacillus paracasei) HD1.7细菌素生成量及群体感应相关基因luxS表达的影响。本研究通过60%硫酸铵沉淀、CM Sepharose Fast Flow弱阳离子交换柱层析中度纯化及Superdex 75凝胶层析精度纯化,将获得的层析分离物与L. paracasei HD1.7共培养,检测共培养发酵液的抑菌活性及luxS基因的转录水平,并用SDS-PAGE与Native-PAGE检测分离物质。结果表明L. paracasei HD1.7抑菌活性为126.68%;luxS基因上调表达,为对照菌株的2.43倍;刺激因子的表观分子量约为30 kDa。本研究利用三步法初步分离纯化出诱导Paracin1.7生成的刺激因子,明确该刺激因子可以启动种间群体感应相关基因luxS,为L. paracasei HD1.7的群体感应研究奠定了理论和技术基础。     

6种钝化剂对糙米镉含量及镉在水稻中的转移和富集的影响

旨在为合理使用钝化剂及水稻糙米镉(Cd)含量与Cd在水稻体内富集和转移的关系提供一定的理论依据。从6种钝化剂中筛选出能够抑制糙米Cd积累的材料,通过对不同超标程度水稻组的产量、糙米Cd含量、各个器官对Cd的富集系数和转移系数进行统计分析,探讨不同钝化剂对Cd在水稻体内转移和富集的影响。结果表明:与对照相比,低、中浓度C材料和FA材料能够降低糙米中Cd浓度;FB材料和中、高浓度FD材料会促使糙米中Cd的积累。水稻各器官对Cd富集能力,无超标水稻为根茎米叶糠,超标水稻为根茎米糠叶;Cd向糙米的转移途径中,在不超标水稻组中糠—米的转移能力最强,在超标水稻组中叶—米的转移能力最强。     

Can additional N fertiliser ameliorate the elevated CO2‐induced depression in grain and tissue N concentrations of wheat on a high soil N background?

Elevated CO₂ stimulates crop yields but leads to lower tissue and grain nitrogen concentrations [N], raising concerns about grain quality in cereals. To test whether N fertiliser application above optimum growth requirements can alleviate the decline in tissue [N], wheat was grown in a Free Air CO₂ Enrichment facility in a low‐rainfall cropping system on high soil N. Crops were grown with and without addition of 50–60 kg N/ha in 12 growing environments created by supplemental irrigation and two sowing dates over 3 years. Elevated CO₂ increased yield and biomass (on average by 25%) and decreased biomass [N] (3%–9%) and grain [N] (5%). Nitrogen uptake was greater (20%) in crops grown under elevated CO₂. Additional N supply had no effect on yield and biomass, confirming high soil N. Small increases in [N] with N addition were insufficient to offset declines in grain [N] under elevated CO₂. Instead, N application increased the [N] in straw and decreased N harvest index. The results suggest that conventional addition of N does not mitigate grain [N] depression under elevated CO₂, and lend support to hypotheses that link decreases in crop [N] with biochemical limitations rather than N supply.     

Effects of bFGF on brain edema,nerve function injury and autophagy related protein in rats with traumatic brain injury

AIM:To study the effects of basic fibroblast growth factor (bFGF) on brain edema, nerve function damage and autophagy related proteins in rats with head injury. METHODS:The rat model of craniocerebral injury (CI) was constructed. The rats were divided into control group, CI group, and low-, middle-and high-dose bFGF groups (n=10). The CI model was established in CI group, while the rats in control group were not given epidural impact. The rats in low-dose, middle-dose and high-dose bFGF groups were given bFGF at 2, 4 and 6 μg, respectively, by intraperitoneal injection after 30 min. The neurological function in the rats was evaluated by improved neurological function scoring. The rat brain tissues were taken, and the water content was detected. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β in the brain tissue were measured by ELISA. The malondialdehyde (MDA) content was analyzed by thiobarbituric acid method. The activity of superoxide dismutase (SOD) was examined by WST-8 assay. The glutathine peroxidase (GSH-Px) activity was detected by colorimetric method. The protein levels of autophagy related proteins LC3-Ⅱ and beclin-1 in the brain tissues were determined by Western blot. RESULTS:The neurological function score was increased significantly of the rats in CI group. The rat model of craniocerebral injury was successfully constructed. Neurological function scores in the rats in low-dose, middle-dose and high-dose bFGF groups were reduced, the water content of the brain tissue was also reduced (P<0.05). The levels of TNF-α, IL-6 and IL-1 β were decreased in the brain tissues (P<0.05), the content of MDA was declined (P<0.05), the activities of SOD and GSH-Px were increased (P<0.05), the protein levels of LC3-Ⅱ and beclin-1 were decreased, compared with the untreated rats in CI group (P<0.05). CONCLUSION:bFGF improves the nerve function of the rats with craniocerebral injury, reduces the water content of the brain tissue, reduces the expression of autophagic protein LC3-Ⅱ and beclin-1.The mechanism is related to the inhibition of inflammatory reaction and oxidative damage.     

小麦TaeEF1β基因的克隆、同源性及表达分析

真核翻译延伸因子(eukaryotic translation elongation factor,eEFs)是一种重要的多功能调控蛋白,eEF1β是eEF1的组成部分,在蛋白质生物合成过程中发挥着重要的作用。本文通过RT-PCR扩增克隆小麦(Triticum aestivum L.)的eEF1β基因,并命名为TaeEF1β。氨基酸同源性分析发现,TaeEF1β具有高度保守性,且其保守结构域位于137~226 aa处。qRT-PCR结果表明,中国小麦花叶病毒(Chinese wheat mosaic virus,CWMV)侵染小麦植株后,可以诱导TaeEF1β基因转录水平的上调表达。另外,本文也进一步分析了TaeEF1β基因在小麦根、茎、叶的表达水平和CWMV侵染不同时间点的表达情况。     

湖南省粮食产量波动的影响因素分析及预测

旨在维护国家稳定,为预判粮食生产前景、提高粮食生产效率、保障粮食安全提供理论依据。利用湖南省统计数据,运用灰色关联分析法筛选关联性较强的影响因素,并建立GM(1,N)预测模型预测粮食产量。2008—2017年与湖南省粮食产量关联度最大的影响因素是粮食作物播种面积和农业机械总动力;科技因素是影响2008—2017年湖南省粮食产量的主要因素,其次是自然因素,社会因素;2018—2027年湖南省粮食产量有较小波动,且农业机械总动力和财政农业支出影响较大;农业机械总动力在前后十年对粮食产量都有较重要的影响,越来越占据主导地位。粮食产量受国家政策的影响,受农业机械总动力影响最大,维持产量水平需高度重视农业机械化水平,稳步提高粮食作物播种面积。     

桑叶养猪实用技术

桑叶养猪实用技术分为桑树种植技术和桑叶干粉发酵养猪技术两个部分。利用荒地种植桑树,降低生猪养殖饲养成本,桑叶养猪种养结合,成为生态农业典范。桑叶干粉发酵技术,提高了桑叶干粉的可消化率和饲料功能。桑叶干粉发酵料占配方6%~10%,做成桑叶猪饲料,替代3%~5%鱼粉、豆粕等蛋白质饲料,节约了饲料成本。桑叶喂养猪(简称桑叶猪,下同)的猪肉中含有肌苷酸等风味物质,显著提高猪肉胴体品质和猪肉风味。设计猪尿处理,采用红泥膜沼气池降解工程工艺应用后,降解水兑2倍清水对桑树喷灌工艺,减少人工处理劳力和成本费用。设计10%抗非洲猪瘟中药饲料添加剂+桑叶饲料,预期防治非洲猪瘟总效果85%以上。桑叶养猪实用技术可行,供同行参考。