Effect of tea-polyphenols on the activation of NF-κB and the expression of TGF-β1 mRNA in THP-1 cells incubated with VLDL,LDL or ox-LDL

AIM:To investigate the effect of tea-polyphenols (TP) on the activation of NF-κB and the expression of TGF-β₁ mRNA in THP-1 cells (a human acute monocytic leukemia cell line). METHODS:THP-1 cells were incubated with the different concentrations of TP, VLDL, LDL or ox-LDL. In the THP-1 cellls, the nuclear malposition rate of NF-κB was detected with immunohistochemistry technique, the positive index of the TGF-β₁ mRNA expression was detected by hybridization in situ, and accumulation of total cholesterol (TC) in cells incubated with 0.4-40 μg/L TP was determined with oxidase assay. RESULTS:The nuclear malposition rate of NF-κB, the positive index of the TGF-β₁ mRNA expression and TC in THP-1 cells incubated with 0.4-40 μg/L of TP were lower than those with 0 μg/L of TP in TP-V group, TP-L group and TP-O (P＜0.05). The differences of these markers in THP-1 cells incubated with more than 40 μg/L TP in TP-V group, TP-L group and TP-O were not statistically significant, compared with TP-C group (P＞0.05). CONCLUSION:TP inhibited the activation of NF-κB, the expression of TGF-β₁ mRNA and the foam cell formation in the mono-macrophage.     

额尔齐斯河流域生态系统格局及变化

本文首先对额尔齐斯河流域气候，地貌，水文等环境因子特征进行了评述，进而阐述了流域生态系统的特征和格局以及在自然和人为干扰作用下的变化。分析结果表明在自然干扰作用下流域生态系统的格局与流域水文情势变化相适应，处于准稳定变化状态，而在人为对流域水文情势扰动下，流域生态系统的格局间接地受到影响。从而产生新的格局，其作用的强度，持久性和生态系统的格局变化程度过错大于大于自然干扰作用下的变化。引额调水工程对额尔齐斯河流域河谷生态系统内部单元格局变化具有重要影响。     

Effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts

AIM: To investigate the effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts. METHODS: In the present experiment, the human vascular fibroblasts were cultured and effects of platelet-derived growth factor-BB on DNA and collagen protein synthesis in human vascular fibroblasts were observed by using [³H]-TdR incorporation and [³H]-proline incorporation in vitro. RESULTS: Platelet-derived growth factor-BB significantly promoted NDA synthesis and collagen protein synthesis of quiescent human vascular fibroblasts, with a maximal response at a concentration of 30μg·L^-1at 24 h and 36 h, respectively. CONCLUSION: Platelet-derived growth factor-BB promotes DNA and collagen protein synthesis in cultured human vascular fibroblasts.     

影响微孢子虫对棉铃虫幼虫致病力的因子

1997年田间调查时，发现一种寄生于棉铃虫的微孢子虫，对棉铃虫具有很强的致病力。为明确环境因子对该微孢子虫及其致病力的影响，测定了温度与紫外线对该微孢子虫及其杀虫效果的影响。结果表明，该微孢子虫孢子耐受温度范围较宽，最高温度上限为55℃。温度对其致病力有显著影响，在一定温度范围内，随着生境温度的升高，微孢子虫对棉铃虫幼虫致病力增强。该微孢子虫对紫外线较敏感，在紫外线照射下易失活而丧失致病力。     

猪胚胎死亡的影响因素及其预防措施

猪胚胎死亡直接影响着母猪的产仔数，并进而影响着养猪生产的经济效益。因此，在深入研究猪胚胎死亡规律及其影响因素的基础上，通过有效的措施降低胚胎死亡率，对提高母猪窝产仔数和经济效益具有十分重要的意义。本文综述了影响猪胚胎死亡的遗传、环境、营养和疾病等因素，并对生产实践中如何通过综合预防措施降低猪胚胎死亡率进行了初步探讨。     

营养元素对木霉菌防治甜瓜枯萎病效果的影响

为明确各种营养元素在木霉菌防治甜瓜枯萎病生防体系中的作用,研究了9种营养元素铜、锌、铁、硼、钼、钙、锰、镁及钾对甜瓜枯萎病菌菌丝生长、木霉菌T23菌丝生长及产孢量、甜瓜种子萌发及根系生长的影响.结果表明:10～1000μg/mL的硫酸铜、硫酸锌、硫酸亚铁及硼酸对镰孢菌菌丝生长有较强的抑制作用,而对木霉菌的抑制作用远小于镰孢菌.适量加入钼酸铵、硫酸亚铁、硫酸钙及磷酸二氢钾有利于木霉菌菌丝生长及孢子形成,对甜瓜种子萌发及胚根生长也有较强的刺激作用.木霉菌结合钼酸铵、硫酸锰和硫酸钙处理土壤能显著降低甜瓜枯萎病的病情指数,相对防效分别为73.95%、73.77%和71.36%.     

陇东黄土高原冬小麦生产农业气象要素分析

对陇东黄土高原半湿润半干旱气候区旱地冬小麦生长期光、热、水三要素与产量进行了相关分析,得出对冬小麦产量起决定作用的农业气象要素为:孕穗—成熟期光照时数,≥0℃积温,开春土壤含水量 返青—孕穗期降水量(R2 3H)。建立了逐步回归方程,Y=-317.138 0.6317X1 0.4647X2。     

Upregulation of neurokinin A levels by nerve growth factor in the experimental asthmatic guinea pig

AIM: To explore the regulatory mechanism of nerve growth factor (NGF) on neurokinin A in the experimental asthmatic guinea pig. METHODS: Radioimmunoassay was used to determine the alteration of neurokinin A levels in the lower respiratory tract and visceral sensory afferent sites while NGF was absent (inhalation of NGF antibody through nasal cavity) in the asthmatic guinea pig. RESULTS: The contents of neurokinin A in the trachea, bronchus, lung, C₇-T₅ spinal ganglia and the correspondent spinal dorsal horn, nodose ganglia and solitary nucleus area in the experimental asthmatic guinea pig with the absent of NGF in the respiratory tract were much lower than those in the asthmatic and control groups (P＜0.01). CONCLUSION: NGF upregulated the contents of neurokinin A in the lower respiratory tract and visceral sensory sites of the experimental asthmatic guinea pig, and both might be involved in the pathogenesis of asthma.