重组截短型ELR1蛋白在昆虫表达系统中的分泌表达与结晶

为获得马传染性贫血病毒单一受体(ELR1)的结晶体,本研究通过PCR方法获得ELR1胞外区基因片段,利用基因重组技术构建包含ELR1胞外区基因的重组杆状病毒表达质粒(Bacmid-ELR1).将其转染至昆虫细胞sf9中进行蛋白表达,western blot检测结果显示ELR1胞外区截短型重组蛋白在昆虫表达系统中获得表达.目的蛋白经亲和层析和凝胶过滤方法纯后化,通过多组结晶条件筛选获得了ELR1胞外区重组蛋白结晶体.本研究为ELR1立体结构和功能的解析提供了重要基础.     

贵州毕节家畜布鲁氏菌病等疫病的监测报告

本文报道了毕节地区近年来布鲁氏菌病、禽流感和马传贫的流行情况调查结果。结论建立在对来自毕节地区各县(市)2183份畜禽血清样品的检测之上。     

鸡传染性贫血病疫苗免疫母鸡后子代雏鸡外周血液T细胞数量及免疫球蛋白含量变化

应用免疫SPA菌体不法和间接ELISE法对鸡传染性贫血病（CIA）疫苗免疫母鸡后，其子代雏鸡外周血液T细胞数量和血清IgG,IgM,IgA含量进行了动态研究。结果发现：CIA疫苗免疫母鸡后，其子代雏鸡外周血液T细胞数量和IgG,IgM,IgA含量均不同和蔼地高于未免疫的相对照雏鸡，表明CIA疫苗免疫母后，其子代雏鸡全身的体液上述各项指标均明显低于疫苗免疫的子代雏鸡，这与CIAV强毒攻击子代雏鸡后，未免疫的子代雏鸡外周血液上述各项指标均明显低于疫苗免疫的子代雏鸡，这与CIAV强毒感染雏鸡后，其免疫器官组织，尤其是胸腺严重损害，淋巴细胞变性坏死子等密切相关，是导致感染鸡免疫功能低下的基础。     

鸡贫血病毒DNA的PCR扩增及其分子克隆

用ＰＣＲ方法扩增了国内分离的鸡贫血病毒（ＣＡＶ）ＳＪ１株的含ＶＰ２、ＶＰ３以及部分ＶＰ１的１５００ｂｐＤＮＡ片段。经限制性内切酶酶切分析，该片段与Ｃｕｘ－１株的酶谱相似。同时，以ｐＵＣ１１９质粒载体克隆了这一ＤＮＡ片段。     

鸡贫血病-法氏囊病联合免疫母鸡后子代雏鸡外周血液T细胞数量及免疫球蛋白含量变化

应用免疫SRA菌体花环法和间接ELISE法对鸡传染性贫血病 (CIA) -传染性法氏囊病 (IBD)联合免疫母鸡后 ,其子代雏鸡外周血液T细胞数量和免疫球蛋白IgG、IgM、IgA含量变化进行了动态研究。结果发现 ,CM -IBD联合免疫母鸡后 ,其子代雏鸡外周血液T细胞数量和IgG、IgM、IgA含量均不同程度地高于未免疫的相应对照雏鸡 ,表明CIA-IBD联合免疫母鸡后 ,其子代雏鸡外周血液体液免疫和细胞免疫功能明显增强。而CIAV、IBDY强毒攻击后 ,未免疫的子代雏鸡 ,其外周血液的上述指标均明显低于疫苗免疫的子代雏鸡 ,表明未免疫的子代雏鸡外周血液的免疫机能降低 ,这与未免疫雏鸡缺乏特异性抗体 ,强毒攻击后 ,雏鸡免疫器官组织广泛损害 ,淋巴细胞变性坏死等有关     

鸡贫血病的最新研究进展

鸡贫血病是由鸡贫血病毒（Chicken anemia virus,CAV)引起的鸡传染性贫血。本病于1979年由日本学者Yuasa等首先报道，并分离到病毒，其后英国、瑞典、德国、加拿大、美国及中国等国家都证实了本病的存在，澳大利亚及其它一些欧洲、非洲、亚洲国家，通过血清学调查，也发现了鸡群广泛感染CAV^[1]。现在已用PCR、核酸探针等多种方法快速诊断该病^[2]。目前，鸡贫血病已被当作重要的世界性禽病。本文就近几年来，在该病的流行病学、致病机理、病原特征、诊断技术及防治方法方面的最新进展作一介绍。     

Anemia and Growth Retardation in Totally Gastrectomized Swine

The effects of total gastrectomy in six young swine were followed and described during an experimental period up to 18 months. Two of the gastrectomized pigs (Nos. 1 and 2) were medicated with cycobemin every second week. Two other gastrectomized pigs (Nos. 11 and 14) were medicated with iron-dextran twice a week. Three non-gastrectomized swine receiving the same feed as the gastrectomized animals were observed as controls for up to eight months.One of the gastrectomized non-medicated pigs (No. 4) did not gain weight, while the other gastrectomized animals had a normal appearance and gained weight but less than the controls. The non-medicated as well as the cycobemin-medicated gastrectomized animals developed a microcytic hypochrome anemia corresponding to the anemia in iron deficiency. Histochemically loss of iron in the depots (liver, spleen, bone marrow and intestine) was demonstrated in the gastrectomized animals except those treated with iron-dextran. Histological and histochemical examinations of the nervous system did not show any abnormalities.     

Procoagulant Microparticles in Dogs with Immune‐Mediated Hemolytic Anemia

Background

Studies of some human prothrombotic diseases suggest that phosphatidylserine‐positive (PS+) and tissue factor‐positive (TF+) microparticles (MPs) might play a role in the pathogenesis of thrombosis or serve as biomarkers of thrombotic risk.

Hypothesis/Objectives

To determine if circulating levels of PS+MP and procoagulant activity (PCA) associated with PS+MPs and TF+ MPs are increased in dogs with IMHA.

Animals

Fifteen dogs with primary or secondary IMHA and 17 clinically healthy dogs.

Methods

Prospective case‐controlled observational study. Circulating PS+MPs were measured by flow cytometry. PCA associated with PS+MPs and TF+MPs was measured by thrombin and Factor Xa generating assays, respectively.

Results

Circulating numbers of PS+MPs were not significantly higher in dogs with IMHA [control median 251,000/μL (36,992–1,141,250/μL); IMHA median 361,990/μL (21,766–47,650,600/μL) P = .30]. However, PS+MP PCA [control median 2.2 (0.0–16.8) nM PS eq; IMHA median 8.596, (0–49.33 nM PS eq) P = .01] and TF+MP PCA [control median 0.0, (0.0–0.0 pg/mL); IMHA median 0.0; (0–22.34 pg/mL], P = .04) were increased. Intravascular hemolysis, which we showed might increase PS+ and TF+MP PCA, was evident in 3 of 5 dogs with PS+MP PCA and 2 of 4 dogs with TF+MP PCA higher than controls. Underlying disease in addition to IMHA was detected in 1 of 5 dogs with PS+PCA and 3 of 4 dogs with TF+MP PCA higher than controls.

Conclusions and Clinical Importance

TF+ and PS+MP PCA is increased in some dogs with IMHA. Further studies that determine if measuring TF+ and PS+ MP PCA can help identify dogs at risk for thrombosis are warranted.     

鸡传染性贫血病毒分子生物学研究进展

对国内外鸡传染性贫血病毒基因组及其编码蛋白等方面的最新研究成果进行了论述。