仙游县农田害鼠发生特点与防治技术研究

研究表明,仙游县为农区鼠害重发区,田间优势种为黄毛鼠,农舍优势种为黄胸鼠,防治适期为4—5月和8—9月两个害鼠活动高峰期;化学药物防治是综合防治的主要措施;准确掌握鼠情动态,选择高效、安全杀鼠剂,采用正确的投饵技术,才能取得理想的灭鼠效果。     

黄葵素对结核分枝杆菌的抑杀作用研究

Objective To evaluate the antituberculous activities of Huangkuisu (HISS). Meth-ods lOStandard human strains of mycobacterium tuberculosis (H37RV) and multi-drug resistant TBstrainswere inoculated in different concentrations of HISS, blank control and positive TB drug culture medium[()observe the growth of mycobacterium tuberculosis and evaluate the antibacterial activity in vitro.②Certain number of standard strains of Mycobacterium tuberculosis H37Rv and drug-resistant strains were injected into the tail vein[()establish a mouse model of mycobacterium tuberculosis infection. The mice were randomly divided into treatment group, rifampicin, HISS treatment group treated lasting for 6 weeks; the control group was given normal saline. The antibacterial activity in vivo were evaluated[()compare the treatment results according[()the pathological changes in lung and spleen tissues (HE staining and acid-fast staining) and charging amount of bacteria. Results lOthe bactreial colonies were grew normally in model group, but were not grew in     

裸鼠作为“活试管”用于兔卵子异体成熟和受精的研究

摘除裸鼠卵巢的同时,在其卵巢囊中正位移人一小块兔的卵巢组织,3周后用激素PMSG和HCG给裸鼠超排,在注射HCG的同时或3h后,用新鲜的兔精液给裸鼠作人工授精,授精后30～80h处死裸鼠,取出输卵管、子宫和植入的兔卵巢块,在6批51只裸鼠中。从17只裸鼠上收集到23个胚胎(1～8细胞阶段)和11个卵母细胞,在植入的兔卵巢块上可见初级、次级和三级卵泡及红体和黄体。     

动物乳腺特异表达载体的构建及其表达特性研究

为了研制动物乳腺生物反应器,用中国奶山羊β-乳球蛋白基因的5′、3′-侧翼区和β-酪蛋白基因的第1内含子构建动物乳腺特异表达载体p205C3.细胞表达试验结果显示,p205C3载体能够指导lacZ基因在SV40 T基因转化的山羊乳腺上皮细胞中表达,不能指导该基因在COS-1细胞和山羊成纤维细胞中表达.将人激肽释放酶(hKLK1) cDNA克隆入p205C3载体,用获得的重组载体p205C3KLK1注射哺乳期小鼠,然后取其心、肝、脾、肺、肾、脑、肌肉、胰腺和乳汁,酶活性测定结果显示,乳汁中的酶活性高达255.65 U·mL-1,其他组织中的酶活性与正常小鼠无差异.结果表明p205C3载体不仅能有效地指导外源基因在山羊乳腺细胞和小鼠乳腺中表达,而且表达具有较好的组织特异性,可以用于动物乳腺生物反应器的研制.     

核酸疫苗SjCA/pc和SjMf1/pc在小鼠体内的表达

为了观察核酸疫苗SjCA／pc和SjMfl／pc在小鼠体内的表达，取昆明鼠，于其后腿外侧肌注免疫SjCA／pc和SjMfl／pc，2d后剖杀动物．取注射部位肌肉作石蜡切片，免疫组化分析，荧光显微镜下镜检。结果表明，SjCA／pc和SjMfl／pc在小鼠注射部位肌肉可见黄绿色荧光，而对照组无此现象。可见，SjCA／pc和SjMfl／pc可在小鼠体内表达。     

过氧化氢对小鼠骨髓微核率和孕鼠胚胎着床及生长的影响

通过体内试验研究了过氧化氢对小鼠骨髓微核率和胚胎着床及孕鼠生长发育的影响。将妊娠的昆明种(KM)小鼠分成5个处理组,分别为环磷酰胺(40.00 mg/kg)、蒸馏水和过氧化氢(500.00,125.00,31.25 mg/kg)组。于妊娠后6~15 d,每天采用灌胃给药,环磷酰胺组于妊娠后11~13 d腹腔一次性注射。每3 d称重1次,于妊娠第18 d称重后剖腹检查。另取雌雄各半KM小鼠分成5个处理组,分别为环磷酰胺、蒸馏水和过氧化氢(500.00,100.00,10.00 mg/kg)组,均采用灌胃染毒,每天1次,连续4 d,第5 d处死小鼠,取股骨髓液常规制片,计算红细胞微核率。结果表明,吸收胎和死胎数蒸馏水组为5.56%,中剂量过氧化氢组增加到19.53%(P<0.01),低剂量组增加到13.14%(P<0.05)。蒸馏水组小鼠嗜多染红细胞微核率为0%~0.002%,过氧化氢组增加到0.017%~0.032%(P<0.01)。在本试验剂量范围内过氧化氢有明显的胚胎毒性和遗传毒性。     

荧光原位杂交(FISH)技术在转基因小鼠检测中的应用

制备转基因小鼠特有的DIG标记探针，应用染色体荧光原位杂交（FISH）技术，对转基因小鼠外周血细胞的遗传性状进行分析，检测其合子类型，以挑选纯合子小鼠用于保种。结果杂合型小鼠61％的细胞有一个荧光信号，而纯合型小鼠约24％的细胞有两个荧光信号，48％的细胞有一个荧光信号，野生型小鼠无荧光信号。因此染色体荧光原位杂交技术可作为检测转基因小鼠遗传特性的一种方法。     

猪流产嗜性衣原体omp-1基因的重组质粒与重组蛋白免疫组合效果

为增强猪流产嗜性衣原体omp-1基因的免疫措施进而筛选出高效新型分子疫苗,本实验将猪流产嗜性衣原体omp-1基因克隆入pcDNA3.1( )载体构建DNA疫苗,初次和二次免疫分别以核酸/重组蛋白(DNA/r-MOMP)、重组蛋白/核酸(r-MOMP/DNA)、重组蛋白 核酸/重组蛋白 核酸(r-MOMP DNA/r-MOMP DNA)、核酸 核酸(DNA/DNA)和重组蛋白 重组蛋白(r-MOMP/r-MOMP)5种组合接种BALB/c小鼠,同时以载体和弱毒为对照。二次免疫后14 d以ELISA和T淋巴细胞增殖实验检测特异性体液免疫和细胞免疫应答水平。结果显示核酸与重组蛋白同时免疫可以诱导产生较高的体液免疫和细胞免疫水平,而DNA/r-MOMP组合的细胞免疫和体液免疫水平高于r-MOMP/DNA组合,单独免疫DNA组或r-MOMP都不能获得好的细胞和体液免疫。     

Further Report on Carcinogenesis or Tumorigenicity of MDCK Canine Kidney Cell(CKC) Lines and Analysis of Their Chromosome Karyotypes

Using Hela cell cultures as positive control and primary canine kidney cell (CKC) or feline kidney cell (FKC) cultures purified in vitro on passage 3 as negative control, the tumorigenicity of Madin-Darby canine kidney (MDCK) cells was tested in ＞273 nude mice, and colony formation in soft agarose and haemagglutination under different concentration of plant lectins of these cells were carried out at the same time. Subsequently, very low tumorigenicity strains of MDCK line were successfully selected; these were evaluated for the production of canine or feline combination viral vaccines, free of infectious agents, and of known cytogenetic and tumorigenic. It is thus evident that MDCK cell of M, JB, JC, WB or H strain can be approved as substrate for the preparation of attenuated viral vaccines, but MDCK cell of YA, YB and KA strains can not be approved as substrate for the preparation of attenuated viral vaccines. The heritable character of these cell sub-lines is comparatively stable, and shows little significant difference between passages.