甘兰夜蛾核型多角体82—17病毒株对裸鼠感染性的研究

甘兰夜蛾核型多角体NPV82—17病毒株,经腹腔、静脉、口服感染裸鼠.被感染裸鼠9—37天的脏器经电镜观察,其肝、脾的枯否细胞和网状细胞的胞浆内都存有1至数个多角体.另将其脏器悬液回接靶昆虫棉铃虫,腹腔和静脉感染率为83—100%,口服感染率为7—77%.     

Introduced house mice Mus musculus: a significant predator of threatened and endemic birds on Gough Island, South Atlantic Ocean?

House mice Mus musculus have successfully colonized many temperate and sub-Antarctic islands that are the location for breeding colonies of millions of seabirds. Unlike other introduced mammals, the impact of house mice on seabirds and endemic birds is believed to have been negligible. The breeding ecology of seabirds breeding on Gough Island, central South Atlantic Ocean, was studied for the first time during September 2000 to September 2001. Breeding success of the endangered Tristan albatross Diomedea (exulans) dabbenena and endangered Atlantic Petrel Pterodroma incerta were 27.3 and 19.9% respectively. Mortality of large Tristan albatross and Atlantic petrel chicks was observed, and the pattern of wounds and observations of feeding indicate that introduced mice were responsible for this predation. Breeding numbers of the endemic Gough bunting Rowettia goughensis are mostly found in upland areas of Gough Island where mice are scarce and are restricted to inaccessible cliffs in the lowlands where mice are abundant. This pattern, together with the high predation rates of artificial-eggs in lowland habitats in comparison to the uplands, strongly suggests that mice constrain the distribution of Gough buntings. The results of this study provide the first evidence for the role of house mice as a significant predator of endangered and endemic birds. Further research is required to determine if the observed levels of mice predation are a regular occurrence.     

转人MCP和CD59双基因小鼠的制备

采用显微混合注射的方法制备转人MCP(膜辅因子蛋白)和CD59(膜反应性溶解抑制物)双基因小鼠,共注射478枚受精卵,移植于21只受体,其中14只受孕,8只受孕小鼠中途流产,从6只受孕小鼠获得18只仔鼠,经检测,其中9只仔鼠单基因阳性(50％),6只仔鼠双基因阳性(33．3％)。结果表明：通过显微混合注射的方法可以获得转人MCP和CD59双基因小鼠。     

新疆荷斯坦牛β防御素5基因的克隆、表达及表达产物的抑菌活性

【目的】克隆和表达新疆荷斯坦牛中性粒细胞防御素5(BNBD5)基因,对BNBD5蛋白进行纯化并分析其抗菌活性。【方法】利用RT-PCR方法扩增新疆荷斯坦奶牛BNBD5的cDNA序列,将BNBD5 cDNA克隆到原核表达载体pGEX-4T-2,测序分析后转化大肠埃希菌BL21(DE3)。经IPTG诱导后,对表达产物进行SDS-PAGE和Western blotting分析;利用GST亲和柱纯化BNBD5蛋白,并进行体外抑菌试验。【结果】通过PCR扩增获得了218bp的牛BNBD5全长cDNA序列,经IPTG诱导,获得了约33 ku的牛BNBD5蛋白。牛BNBD5蛋白在15~25℃培养时的可溶性较37℃时多,且大多以包涵体形式存在,经纯化后最终获得了少量的目的蛋白。纯化的BNBD5蛋白对标准金黄色葡萄球菌和标准大肠埃希菌均具有明显的抑菌活性。【结论】克隆、表达了牛BNBD5基因,表达的BNBD5蛋白对大肠埃希菌和金黄色葡萄球菌都有抑菌活性。     

Cellular immune responses of BALB/c mice induced by intramuscular injection of PRRSV-ORF5 DNA vaccine with different doses

BALB/c mice were immunized with 50 μg, 100 μg, 200 μg of pcDNA-PRRSV-ORF5 DNA vaccine respectively by intramuscular injection, with PBS and pcDNA3.1(+) as controls. Fluorescence activated cell Sorter (FACS) was used to detect the number of CD₄ ⁺ and CD₈ ⁺ T-lymphocytes. T-lymphocyte proliferation test was used to detect proliferation of the T-lymphocyte cells in peripheral blood lymphocytes of mice vaccinated with pcDNA-PRRSV-ORF5 DNA vaccine. The results showed that the difference in ConA response to T-lymphocytes in blood was highly significant between all experimental groups and the control group (P < 0.01). The number of CD₄ ⁺ T-lymphocytes in experimental groups was significantly higher than that of the control group 7 d after vaccination. The number of CD₈ ⁺ T-lymphocytes in the experimental groups was higher than that of the control group 28 d after vaccination. Mice immunized with a higher dose (200 μg) of DNA vaccine demonstrated higher cellular immune response than those immunized with a lower dose (100 μg, 50 μg) of DNA vaccine. The results demonstrated that pcDNA-PRRSV-ORF5 DNA vaccine could induce a good cellular immune response which may be dose-dependent. __________ Translated from China J Vet Sci Mar, 2006, 26(2): 111–114 [译自: 中国兽医学报] The first three authors contribute equally to this work.     

初生仔猪肠管数据的相关回归分析

取32例初生仔猪,分离其小肠、大肠,并将血管用乳胶灌注,然后测量其体长、小肠、大肠长度及口径以及分布到小肠、大肠上的动脉分枝数目。利用计算机处理得出:小肠长度与直径之间,体长与小肠长度之间,体长与小肠直径之间,小肠的1级分枝与终末枝之间,小肠长度与1级分枝之间,小肠长度与终末分枝之间有相关性;但是小肠直径与动脉分枝间无相关性。大肠长度与直径之间,体长与大肠长度之间,体长与大肠直径之间、大肠的长度与终末枝之间、大肠的直径与终末枝之间有相关性。     

黄葵素对结核分枝杆菌的抑杀作用研究

Objective To evaluate the antituberculous activities of Huangkuisu (HISS). Meth-ods lOStandard human strains of mycobacterium tuberculosis (H37RV) and multi-drug resistant TBstrainswere inoculated in different concentrations of HISS, blank control and positive TB drug culture medium[()observe the growth of mycobacterium tuberculosis and evaluate the antibacterial activity in vitro.②Certain number of standard strains of Mycobacterium tuberculosis H37Rv and drug-resistant strains were injected into the tail vein[()establish a mouse model of mycobacterium tuberculosis infection. The mice were randomly divided into treatment group, rifampicin, HISS treatment group treated lasting for 6 weeks; the control group was given normal saline. The antibacterial activity in vivo were evaluated[()compare the treatment results according[()the pathological changes in lung and spleen tissues (HE staining and acid-fast staining) and charging amount of bacteria. Results lOthe bactreial colonies were grew normally in model group, but were not grew in     

核酸疫苗SjCA/pc和SjMf1/pc在小鼠体内的表达

为了观察核酸疫苗SjCA／pc和SjMfl／pc在小鼠体内的表达，取昆明鼠，于其后腿外侧肌注免疫SjCA／pc和SjMfl／pc，2d后剖杀动物．取注射部位肌肉作石蜡切片，免疫组化分析，荧光显微镜下镜检。结果表明，SjCA／pc和SjMfl／pc在小鼠注射部位肌肉可见黄绿色荧光，而对照组无此现象。可见，SjCA／pc和SjMfl／pc可在小鼠体内表达。     

荧光原位杂交(FISH)技术在转基因小鼠检测中的应用

制备转基因小鼠特有的DIG标记探针，应用染色体荧光原位杂交（FISH）技术，对转基因小鼠外周血细胞的遗传性状进行分析，检测其合子类型，以挑选纯合子小鼠用于保种。结果杂合型小鼠61％的细胞有一个荧光信号，而纯合型小鼠约24％的细胞有两个荧光信号，48％的细胞有一个荧光信号，野生型小鼠无荧光信号。因此染色体荧光原位杂交技术可作为检测转基因小鼠遗传特性的一种方法。     

Further Report on Carcinogenesis or Tumorigenicity of MDCK Canine Kidney Cell(CKC) Lines and Analysis of Their Chromosome Karyotypes

Using Hela cell cultures as positive control and primary canine kidney cell (CKC) or feline kidney cell (FKC) cultures purified in vitro on passage 3 as negative control, the tumorigenicity of Madin-Darby canine kidney (MDCK) cells was tested in ＞273 nude mice, and colony formation in soft agarose and haemagglutination under different concentration of plant lectins of these cells were carried out at the same time. Subsequently, very low tumorigenicity strains of MDCK line were successfully selected; these were evaluated for the production of canine or feline combination viral vaccines, free of infectious agents, and of known cytogenetic and tumorigenic. It is thus evident that MDCK cell of M, JB, JC, WB or H strain can be approved as substrate for the preparation of attenuated viral vaccines, but MDCK cell of YA, YB and KA strains can not be approved as substrate for the preparation of attenuated viral vaccines. The heritable character of these cell sub-lines is comparatively stable, and shows little significant difference between passages.