Fusarium head blight and mycotoxin contamination of wheat,a review

Summary An infection of bread wheat by fusarium head blight contaminates the crop with mycotoxins, particularly deoxynivalenol (DON) and nivalenol (NIV). The toxicity and natural occurrence of these mycotoxins in wheat are reviewed. Based on 8 years data of fusarium head blight epidemics of wheat in the Netherlands, DON contamination of the grain was estimated. Fusarium head blight ratings averaged an infection of 1.7% of all spikelets; estimates for DON contamination averaged 0.9 mg kg^–1. Taking a guideline level for DON in uncleaned bread wheat of 2 mg kg^–1, in 1979 and 1982 a wheat crop was produced with estimated DON concentrations above the limit of tolerance. Human and animal exposure to mycotoxins in the Netherlands appears to be small but chronic. The information presented in this paper illustrates the need for an annual evaluation of the crop for fusarium head blight incidence and mycotoxin content, and the necessity of fusarium head blight resistant wheat cultivars.Samenvatting Aaraantasting van tarwe doorFusarium culmorum enFusarium graminearum leidt tot vorming van mycotoxinen in het graan, waarvan deoxynivalenol (DON) en nivalenol (NIV) de belangrijkste toxinen zijn. In dit artikel wordt een overzicht gegeven van de toxicologische aspecten, en het voorkomen van deze toxinen in tarwe. Informatie over DON en NIV in tarwe in West-Europa is schaars. Gebaseerd op gegevens vanFusarium epidemieën in de jaren 1979–1986 wordt een schatting gegeven van de concentratie DON in Nederlandse tarwe. Rekening houdend met de herkomst en verwerking van tarwe, blijken zowel in dierlijk als menselijk voedsel lage concentraties DON chronisch voor te komen. Op basis van een maximaal toelaatbare dagelijkse dosis DON van 3 g kg^–1 lichaamsgewicht is de schatting van de dagelijkse opname van DON in het jaar volgend op de oogst van 1982 net op de grens. Zowel een jaarlijkse inventarisatie vanFusarium aantasting en DON besmetting van het graan, als de ontwikkeling vanFusarium-resistente rassen zijn noodzakelijk.     

杀菌剂对玉米穗腐病菌的毒力及毒素产生的影响

为筛选防治玉米穗腐病同时减少病原菌毒素积累的化学药剂,采用菌丝生长速率法、涂布平板法分别测定了4种药剂多菌灵、百菌清、吡唑醚菌酯和氰烯菌酯对玉米穗腐病的3种主要致病镰孢菌拟轮生镰孢菌、层出镰孢菌和禾谷镰孢菌菌丝生长和孢子萌发的影响,并采用高效液相色谱法测定了4种药剂对其毒素积累的影响。结果表明:层出镰孢菌菌株127-1和禾谷镰孢菌菌株125-3的最佳产毒时间为第10天。供试4种药剂对3种镰孢菌菌丝生长的EC50值范围为0.18~5.71μg/m L,对孢子萌发的EC50值范围为0.02~8.14μg/m L,均表现出一定的抑制作用。但对3种镰孢菌产伏马毒素、玉米赤霉烯酮和脱氧雪腐镰孢菌烯醇的影响不尽相同,吡唑醚菌酯浓度在1~500μg/m L时能够抑制拟轮生镰孢菌和层出镰孢菌产生伏马毒素,氰烯菌酯能够在0.05~0.8μg/m L浓度范围内显著抑制禾谷镰孢菌产生玉米赤霉烯酮,当多菌灵浓度为0.05~0.5μg/m L时,几乎可以完全抑制禾谷镰孢菌产生脱氧雪腐镰孢菌烯醇。     

霉菌毒素污染日粮对后备母猪生长性能、养分消化及血液指标的影响

文章旨在评估呕吐毒素污染的玉米对后备猪生长性能、养分消化及血液指标的影响。试验选择平均初始体重为（33.77±0.23）kg的二元后备猪320头，随机分为4组，每组4个重复，每个重复20头。对照组饲喂正常玉米～豆粕型日粮，处理组饲喂分别用100、200和300 g/kg呕吐毒素污染的玉米替代正常玉米配制日粮，试验共进行14 d。结果：随着日粮毒素污染玉米添加量的升高，对照组1～14 d后备猪的平均日增重较其他组分别提高7.56%、8.32%和14.46%（P＜0.05），同时对照组后备猪1～14 d采食量较T2和T3组显著提高了2.86%和3.94%（P＜0.05），料重比较T3组显著降低了8.97%（P＜0.05）。对照组14 d后备猪阴户宽度较其他组分别显著提高了9.01%、13.74%和11.44%（P＜0.05）。处理组血清IgM浓度较对照组分别显著提高了25.05%、29.65%和25.85%（P＜0.05），血清尿素氮浓度较对照组分别显著提高了30.91%、21.77%和25.19%（P＜0.05），血清谷丙转氨酶（ALT）活性较对照组分别显著提高了33.50%、27.34%和30.46%（P＜0.05）。结论：在本研究条件下，饲喂呕吐毒素污染玉米对后备猪生长性能的负面影响主要表现为采食量和饲料效率降低，但对养分表观消化率无负面影响。     

Acute hepatic necrosis and death in a subadult southern white rhinoceros (Ceratotherium simum) associated with exposure to sterigmatocystin in forage contaminated with Aspergillus nidulans

A young male southern white rhinoceros (Ceratotherium simum), which was resident in a zoo as part of a multi‐rhinoceros group, died suddenly. Necropsy and histopathological findings supported a diagnosis of death from acute hepatic necrosis. The microscopic distribution of liver lesions was suggestive of hepatotoxicosis. Further investigation revealed potential exposure to a mycotoxin, sterigmatocystin, present in spoiled lucerne hay contaminated with Aspergillus nidulans. It was concluded that mycotoxicosis was the likely cause of the hepatic necrosis and death in this animal.     

霉菌毒素免疫抑制作用的研究进展

霉菌毒素是霉菌的有毒代谢产物,对动物机体最重要的危害作用就是能够抑制动物机体的免疫机能,引起动物生长缓慢,生产性能下降,对疾病的抵抗能力降低,造成长远的经济失。同时霉菌毒素之间还有对免疫系统的协同抑制作用。作者针对饲料中几种主要的霉菌毒素对动物机体免疫抑制作用作一综述。     

Ochratoxin A cytotoxicity on Madin–Darby canine kidney cells in the presence of alpha‐tocopherol: Effects on cell viability and tight junctions

Ochratoxin A (OTA) is a potent nephrotoxic fungi metabolite that affects animal and human health. At the cellular level, OTA is able to alter functions and viability by several mechanisms of action. Several strategies to counteract its toxicity have been studied. We investigated the role of α‐tocopherol in counteracting OTA oxidative damage in Madin–Darby canine kidney (MDCK) cells by pre‐incubating the cells for 3 hr with the antioxidant (1 nm , 10 μm ) and then adding OTA (0–1.2 μg/ml) for the following 24 hr. Cell viability, lactate dehydrogenase (LDH) release, TUNEL staining and occludin and Zo1 localization by immunofluorescence were determined. Here, 1 nm α‐tocopherol was shown to significantly reduce (p < .05) the cytotoxicity, LDH release and apoptotic rate induced by OTA. The presence of the antioxidant at the same concentration maintained the localization of occludin and Zo1 in the rim of the MDCK cells after the 24‐hr OTA exposure. These results indicate that a low concentration of α‐tocopherol could block OTA toxicity, supporting its defensive role in the cellular membrane.     

Mycoflora and Mycotoxicological Quality of Four Freshly Harvested Paddy Rice Cultivars and Relation with Harvest to Industry Reception Timing

Grain quality and safety of four freshly harvested paddy rice (Oryza sativa L.) cultivars (Epagri 109,SCS 114 Andosan,Tio Taka 113 and Epagri 115 CL) grown under irrigation system in the north of Santa Catarina State,Brazil were studied.The quality parameters evaluated were mycoflora,mycotoxins (aflatoxins,ochratoxin A and zearalenone),water distribution (moisture content and water activity) apart from environmental conditions (relative humidity and temperature) and the relation between harvest and the industry reception timing.All cultivars presented an average waiting time of 8.6 h (ranged from 0.3 to 24.9 h),a total fungi load of 8.6×104 cfu/mL (ranged from 1.8×103 to 9.4×105 cfu/mL) with a rather high humidity condition of 19.0% moisture content (varied from 14.9% to 24.5%) and 0.92 water activity (varied from 0.78 to 0.99) under the average environmental conditions of 87.3% relative humidity (varied from 80.0% to 96.8%) and 25.4 ℃ (varied from 22.5 to 27.5 ℃),respectively.The conditions to which the samples were submitted allowed fungal growth,but no mycotoxins were detected.Regarding the rice cultivars fungal genera isolated,Aspergillus and Penicillium were the most often isolated among all the cultivars and variation on fungi distribution among them was not observed.Moreover,it was not found a correlation of the grain loading & unloading time with the humidity conditions and the total fungi load.Furthermore,all results and the importance of monitoring the rice mycotoxicological quality and safety were discussed.     

国内外饲料真菌毒素限量规定及评析

真菌毒素污染饲料可对人类和动物健康造成严重危害。本文对我国及美国、欧盟、加拿大等主要贸易国家和地区发布的饲料真菌毒素法规或卫生标准进行整理和比较分析,针对如何进一步完善我国饲料卫生标准和法规体系以及加强进出口把关进行探讨并提出建议。     

Virulence and mycotoxic effects of Metarhizium anisopliae on Mahogany shoot borer, Hypsipyla robusta (Lepidoptera: Pyralidae)

Developing appropriate control measures for the Mahogany shoot borer,Hypsipyla robusta Moore has become increasingly important due to the severe damaging effect of the pest on the establishment of the saplings of Swietenia mahagoni Jacq(Sapindales:Meliaceae).Existing management methods are largely limited to silvicultural practices and spraying of chemical insecticides.To identify a potential fungal biocontrol agent,we compared the virulence of six native and two standard ARSEF isolates of Metarhizium anisopliae Metsch.against this pest.The average survival time and conidial yield of IWST-Ma7 was higher(6.2 to 7.3 days and 4.9 to 4.7 × 10 5 conidia/ml) than the standards.Significant difference in sporulation on the cadavers between isolates,doses and incubation periods were substantiated for the selection of potential strain.The mycotoxic effects of crude soluble protein extract when incorporated in the artificial diet,the ARSEF 2596 and ARSEF 3603 showed LD 50 value of 3.7% and 5.6%.However,IWST-Ma7 was highly lethal with significant lowest LD 50 value of 2.6%.The enzyme activity of IWST-Ma7 was highest for chitinase,CDA,protease and lipase viz.,1.90 U/mg,1.80 U/mg,0.98 U/mg and 0.80 U/mg respectively.However the enzyme activity of chitinase and Chitin deacetylase assay for all the isolates was significantly higher than protease and lipase activity.The ITS regions(5.8S rDNA and 28S rDNA) of seven isolates of M.anisopliae were amplified using the ITS1 and ITS4 primers which was a unique fragment of approximately 550 bp.Based on ITS regions,phylogenetic tree have been constructed and the isolates have been grouped in to 5 clades.The virulence and mycotoxic effects of different isolates could rationally be used to employ them for the management of the mahogany borer.