肺炎克雷伯氏菌致白颊长臂猿脓肿一例

白颊长臂猿是中、老、越三国交界地区的特有种,属于国家Ⅰ级重点保护动物。目前我国白颊长臂猿主要分布于西双版纳自然保护区和绿春黄连山自然保护区。北京动物园目前共饲养了十几只白颊长臂猿,繁殖状况良好。2009年9月我园1只白颊长臂猿体表出现多处脓肿。后抽取脓液,分离到1株革兰氏阴性小杆菌。该白颊长臂猿经治疗无效后死亡。剖检时发现其体内有亦有大量脓液,经分离培养,与体表分离到的是同一株细菌。该菌株经鉴定是肺炎克雷伯氏菌肺炎亚种（Klebsiella pneumoniae ssp pneumoniae）。并对其进行了药物敏感试验。     

Mycoplasma suipneumoniae and mycoplasma flocculare in the growth precipitation test.

A number of laboratory and field strains of Mycoplasma suipneumoniae and Mycoplasma flocculare were subjected to a comparative examination by the growth precipitation test. It was found that all the strains could readily be identified by that test. Slight evidence of cross-reaction was noted for a few of the laboratory strains, but not until late in the observation period. Only some of the field strains would form precipitates when primary cultures (from tissue suspensions) were used, but all strains could be identified already in the second and third passages. The test therefore seems well suited for distinguishing the two species from each other.     

一株七彩鸟肺炎克雷伯菌同时产TEM和SHV型β-内酰胺酶的基因型鉴定

用全自动细茵鉴定系统鉴定分离菌,用表型确证试验法检测超广谱β-内酰胺酶(ESBLs),并分别用TEM、SHV、CTX-M 3种特异性引物进行PCR扩增、基因克隆及测序分析,确定分离茵所产β-内酰胺酶的基因型和基因亚型.结果表明:分离茵为产ESBLs的肺炎克雷伯菌,具有多重耐药特性,其质粒上具有TEM序列和SHV序列,基因亚型分别TEM-1型和SHv-11型.     

Chlamydia pneumoniae enhanced the expression of ICAM-1 in cultured human umbilical vein endothelial cells

AIM: To investigated the effects of Chlamydia pneumoniae (C.pneumoniae) infection on the expression of intercellular adhesion molecule-1 (ICAM-1) in cultured human umbilical vein endothelial cells (HUVECs). METHODS: After propagated in HEP-2 cells, C. pneumoniae organisms were infected to HUVECs. The infection was assessed by ectromicroscope and PCR. The expression of ICAM-1 on HUVECs was detected by flow cytometry before infection and 12 h, 24 h, 48 h, 72 h after infection. RT-PCR was used to detect the ICAM-1 mRNA expression. RESULTS: C.pneumoniae was able to infect cultured HUVECs. After infection, the expression of ICAM-1 on the surface of cultured HUVECs was increased,, the peak was at the time of about 24-48 h; The light quantative RT-PCR analysis demonstrated that the infection also enhanced the ICAM-1 mRNA expression. CONCLUSION: The ability of C.pneumoniae to grow in HUVECs and to stimulate the expression of ICAM-1 protein and mRNA suggests that C.pneumoniae may playa role in atheriosclerosis.     

大熊猫肺炎克雷伯氏杆菌的分子诊断

建立了PCR分子诊断技术 ,并对大熊猫主要肠道病原菌肺炎克雷伯氏杆菌进行检测。结果表明 ,该方法简单、快速、敏感、结果可靠 ,适用于大熊猫肠道病原菌的早期诊断 ,该方法具有广阔的应用前景。     

猪肺炎霉形体及其膜致细胞病变的研究

采用培养的兔肾原代单层细胞，接种猪肺炎霉形体及其膜部分，观察其致细胞病变情况，当霉形体接种量为５～１５μｇ／ｍｌ菌体蛋白时，无细胞病变作用，而接种量为２０～３０μｇ／ｍｌ菌体蛋白时，均有致细胞病变作用；当霉形体膜接种量为５～２５μｇ／ｍｌ膜蛋白时，无细胞病变作用，接种量达３０～５０μｇ／ｍｌ膜蛋白时，均可使细胞病变。随着接种量的加大和作用时间的延长，致细胞病变的程度增强。     

水竹丛枝病的研究——Ⅰ.症状和病原

对水竹丛枝病的症状和病原进行观察研究表明:水竹丛枝病的典型症状是病枝节间缩短,侧枝、细枝丛生。每年从4月起,在病枝叶鞘内产生白色米粒状子座,5月中旬开始在其外围产生褐色垫状子座:即瘤座菌的有性世代;通过电镜观察,发现在病株同一组织的超薄切片中,同时存在类细菌,类菌原体和真菌菌丝,其中真菌菌丝的形态和大小与病株叶鞘内的瘤座菌子座切片菌丝相似。用分离培养的类细菌培养液针注接种能诱发致病,其症状与自然发病相同。进行再分离,其菌落形态、菌体大小和形态结构均与接种物相似。     

仔猪克雷伯氏菌病的病原分离鉴定及其疫苗制备

克雷伯氏杆菌既是一种人兽共患病病原又是一种可引起多种动物患病的条件性致病菌。常可引起仔猪败血性克雷伯氏杆菌病,引起仔猪大量死亡,本文对临床采集的病料进行镜检、细菌分离培养、生化特性和致病性分析后分离出克雷伯氏菌;同时制备了自家氢氧化铝灭活苗,并对疫苗的安全性、保护性及免疫性进行了检测。结果表明:利用当地发病猪群分离的克雷伯氏杆菌制备的灭活苗性能良好使用安全可靠。     

猪肺炎霉形体细胞膜的制备方法

本文针对常规低渗破膜效率较差这一特点，从膜纯度，膜产量，膜毒力三方面入手进行了三种细胞裂解方法的比较研究。表明：超声法膜蛋白产量和膜ＡＴＰａｓｅ活性分别比低渗膜提高１０．１３％、２９．６％，但膜毒性降低；低渗超声膜相应指标分别比低渗膜提高１６．７９％，２．９８％，而且膜毒性也有所改观，对猪肺炎霉形体是一种比较理想的细胞裂解方法     

Detection of MLOs in declining alder trees in Southern Italy and their characterization by RFLP analysis

Mycoplasma-like organisms (MLOs) were detected and characterized by RFLP analysis in declining trees of Alnus glutinosa and A. cordata in Southern Italy for the first time. The infected trees showed symptoms of yellowing, sparse foliage, premature autumn colouration, sprouting, deliquescent branching, phloem necrosis, dieback and witches’ brooms.