默顿职业兴趣的汇聚与转移理论述评

职业兴趣的汇聚与转移是默顿博士论文的第一部分。从科学发展的外部因素来理解科学是默顿论文的意义所在,他对职业兴趣的汇聚与转移的分析恰好为其找到了研究的突破口。用社会学方法来研究科学的发展,虽有其成功的一面,也有其局限性。     

基于SRAP分子标记的11份柑橘种质材料遗传多样性分析

为分析11份柑橘种质材料的遗传多样性,本研究以7份沙糖橘种质以及4份其他柑橘种质为试验材料,利用SRAP分子标记进行分析,并用UPGMA法进行聚类.结果表明:160对引物组合筛选出20对引物组合扩增条带清晰,11份种质材料两两之间遗传相似系数在0.322 1～0.931 6之间.沙糖橘和金葵蜜橘间的遗传相似系数最大,为...     

立式金属油罐容量检定中的几个问题

针对JJG168-87计量规程在实际使用中的缺陷，对罐壁圈板径向偏差测量位置的确定，油罐充满油品后直径增大值的修正等问题，提出了具体的解决方法。     

TEOM1405F型PM2.5颗粒物分析仪校准方法的建立

对美国赛默飞世尔公司TEOM1405F颗粒物分析仪制定合理可行的校准方法,并且根据标准方法对仪器进行检定.校准结果为:环境温度传感器误差小于±2℃,环境气压传感器误差小于1 kPa,采样流量相对误差小于±3％,弹性常数K0＜±2％,绝缘阻抗＞20MΩ,满足校准方法技术要求.根据检定结果,该仪器监测数据准确可靠.     

甘蔗线虫病抗性基因的PCR检测研究

以38份未经线虫病常规鉴定的甘蔗种质资源为供试材料,根据番茄抗根结线虫病基因和甜菜抗胞囊线虫病基因的保守序列区域,分别设计了2条上游引物、2条下游引物,对设计的引物进行组合后,应用PCR特异扩增从中分别筛选出各1对特异引物。用抗根结线虫基因设计的特异引物进行扩增最终获得了1条大约460 bp大小的特异片段;用抗胞囊线虫基因设计的特异引物进行扩增最终获得了1条大约690 bp大小的特异片段,进一步进行了PCR-Southern杂交,确认了该2条特异引物的真实性。     

Calculation Method of Hydraulic Characteristics for Direct-Return Chilled Water System

In order to optimize design and operation, it is necessary to calculate accurately hydraulic characteristics large central air-conditioning systems. Simplified Model can not be applied to hydraulic calculation of large pipe network topological structure for its large calculation error. Taking into account the regulating characteristics of terminal branch temperature regulating valve, a direct-return chilled water system is studied, an accurate mathematical model of pipe network hydraulic characteristics has been established and a computer logic algorithm with virtual flow has been given. A direct-return pipe network with ten AHU branches is used as simulation calculation object, the pipe network supply-return water pressure difference is calculated, and also the temperature regulating valve opening and actual flow of each branch are calculated in the condition of different supply-return water pressure difference. The calculated results accord with the natural characteristics of high pressure difference unbalanced for direct-return pipe network. The calculation method can ensure calculation convergence.     

Quantitative PCR assays to enumerate Rhizobium leguminosarum strains in soil also target non viable cells and overestimate those detected by the plant infection method

The plant infection method is commonly used to estimate the Most Probable Number (MPN) of soil rhizobia. Here, a qPCR method was set-up and validated with newly developed ANU (strain specific) and RHIZ (more general) primers to quantify the specific Rhizobium leguminosarum bv. trifolii ANU843 strain or general R. leguminosarum strains. Detection limits of qPCR protocols in soil were 1.2 × 10⁴ (ANU) and 4.2 × 10³ (RHIZ) cells per g soil. The qPCR assay appears robust and accurate in freshly inoculated soils but overestimated MPN for indigenous soil rhizobia. An incubation experiment showed that qPCR detected added DNA or non viable cells in soils up to 5 months after addition and incubation at 20 °C in moist conditions.     

高酸值米糠油酶法酯化脱酸研究

对脂肪酶Novozym 435催化高酸值米糠油酯化脱酸进行了研究.利用响应面分析法(RSM)优化脱酸条件.根据Box-Benhnken中心组合试验设计原理对试验条件进行优化,在分析各因素显著性及其交互作用的基础上,得出米糠油酯化脱酸最佳工艺参数为:酶质量分数1.1％、甘油添加量0.42 mg、温度56.4℃、反应时间23.2 h.实际测得脱酸后米糠油酸值(KOH)由56 mg/g降为5.04 mg/g,游离脂肪酸酯化率达到91％,与模型预测值基本相符.     

快速提取分离八角茴香油的新方法

将回流提取法、水蒸气蒸馏法、快速提取法对八角枝叶或干果中八角茴香油提取进行了比较,并确定八角枝叶或干果中八角茴香油的最佳提取方法和条件。结果表明,快速提取法比回流提取法、水蒸气蒸馏法提取效率高,快速简便。快速提取法提取八角茴香油的最佳条件为:干果原料与水比为1∶6,提取回流时间为2 h,产品收率达12.78%,且含八角茴香脑90.83%。