The complete mitochondrial genome and molecular phylogeny of Lueyang black-bone chicken

ABSTRACT

1. The objectives of the current study were to investigate the mitochondrial genome and molecular phylogeny of Lueyang black-bone chicken, and provide molecule base to preserve and explore the specific chicken strain.

2. Based on sequencing and clustering, the complete mitochondrial DNA map and sequences of Lueyang black-bone chicken were revealed, and two phylogenetic trees of Lueyang black-bone chickens based on D-loop sequences and the mitochondrial genome were constructed.

3. The results showed that the complete mitochondrial genome of Lueyang black-bone chickens is 16,784bp in size, consisting of 22 transfer RNA genes, two ribosomal RNA genes, 13 protein-coding genes, and one non-coding control region. The base composition of the complete mtDNA sequence is 30.28% for A, 23.78% for T, 32.42% for C, 13.52% for G. Additionally, 10 haplotypes of D-loop sequences in 32 Lueyang black-bone chickens were detected, which were distributed into 4 clades (A, B, C and E).

4. It was concluded that genetic diversity is wide in Lueyang black-bone chickens, and this strain has multiple maternal origins from different regions in China and neighbouring regions.     

VEGF在绵羊生殖管道中的表达规律

以不同发情周期雌性绵羊子宫、输卵管为研究对象,采用免疫组织化学技术,针对血管内皮生长因子（VEGF）在绵羊子宫、输卵管的表达、定位和变化规律进行了检测,同时应用相关图像分析软件对抗原染色强度进行了定量分析。结果表明：输卵管在发情0～15d,VEGF表达量在第9天达到峰值后经历波动逐渐下降过程,输卵管内膜上皮细胞是VEGF抗原的主要靶细胞;而子宫角在发情0～15d,VEGF表达量在第5天达到峰值后经历波动逐渐下降过程,子宫内膜固有层及腺体周围细胞为VEGF抗原的主要靶细胞。该研究结果为绵羊生产中进一步提高受胎率和妊娠率及频密产羔等技术的应用提供了科学依据。     

Determining spatial feed distribution in sea cage aquaculture using an aerial camera platform

Within this paper we describe an unmanned aerial vehicle (UAV) based method to estimate the spatial feed pellet distribution in salmon fish-cages and exploit it within a case study to determine the radial pellet distribution for different rotor spreaders and blower configurations. Compared to previously used methods, capturing pellets thrown from a rotary feed spreader in rows of Styrofoam boxes, the UAV based method is simpler and faster to setup and allows to cover a larger portion of the sea cage surface area. We compare results obtained with the Styrofoam box method with results we obtained by an automatic analysis of aerial videos taken by the UAV during feeding experiments. The employed method helps to gain insight into the spatial feed pellet distribution in full-scale salmon fish-cages where the feeding pipe and actual fixation of the spreader may influence the dynamic behaviour of the feeding system. This can also be seen as a step towards a continuous measuring of the feed pellet distribution, particularly in view of possible future feeding systems that may allow to adapt the feed pellet distribution to the actual fish distribution.     

安徽省农业气象灾害时间分布特征与灰色关联分析

为了探寻安徽省农业气象灾害的分布特点以及各种气象灾害对粮食生产的影响,本研究基于安徽省1992—2012年有关气象灾害数据,对4种主要气象灾害(旱灾、水灾、风雹灾、霜冻灾)的分布特征进行统计分析,并采用灰色关联分析研究其对安徽省粮食单产的影响。结果表明:1992—2012年,安徽省气象灾害具有发生频率高、波动大的特点,但整体上呈下降趋势。旱灾和水灾是发生面积较大的气象灾害,且常在时间上交织、空间上并存。灰色关联分析表明,4种气象灾害对粮食产量影响顺序:风雹灾水灾旱灾霜冻灾,说明风雹灾是影响粮食产量最主要的气象灾害,其次是水灾、旱灾,霜冻灾影响较小。本研究可为安徽省防灾减灾措施的制定提供决策依据。     

基于GIS的农村居民点空间分布特征研究----以扶余市为例

科学认识乡村地域结构是全面推进农业农村现代化和乡村振兴的基础。本文以扶余市第二次土地调查调数据为基础，借助ArcGIS空间分析软件，运用规模分析、核密度估算、缓冲区分析，从用地规模、分布格局与土地利用条件方面探讨扶余市农村居民点地域分异特征。研究表明：扶余市以微、小型居民点居多，居民点数量随着规模的增大逐渐减少，居民点之间的距离差异较大，总体呈现大散居、小聚居，不均匀分散分布；农村居民点的平均核密度为0.4个/km2，空间布局呈东南部高密度集聚成片，向西逐渐密度降低，西部零散错落分布；居民点分布呈现较强的城镇指向性，交通用地对居民点有集聚的作用，河流对微型居民点和大型居民点有集聚吸引的功能。     

基于MCMC模拟的MGPD模型及其在地质灾害风险度量中的应用

基于马尔科夫链蒙特卡洛(简记为MCMC)模拟的参数贝叶斯估计,对改进的广义帕累托分布(简记为MGPD)模型进行了优化,并利用该模型得到了地质灾害损失的在险损失值(简记为VaR)和条件损失值(简记为CVaR).以湖南娄底市地质灾害损失数据进行实证分析及模型适应性检验,结果表明:优化后的模型不仅具有很好的极值数据描述能力,而且具有较强的适用性.     

基于图书出版发行现状的图书馆采访工作创新

本文着重分析了当前我国图书出版发行环境中出现的一些新的变化、新的特点和新的问题,并提出了在图书馆采访工作中,针对这些问题可采取相应的措施。     

The Relationship Between Vegetation Characteristics and Altitudes in Transitional Permafrost Zone in Xidatan,Qinghai-Tibetan Plateau

Many studies showed that permafrost has profound influence on alpine ecosystem. However, former researches were mainly focused on typical points by temporal scales. There were few studies about the correlation between vegetation characteristics and different altitudes covering a large region in spatial pattern, especially in transitional permafrost(TP). There were continuous permafrost(CP) discontinuous permafrost(DCP) and seasonal frozen ground(SFG) in this study region. The types of permafrost changed from SFG to DCP, and finally become CP as the altitudes of Xidatan increase. In this paper, 112 845 points interpreted by HJ1-B(environment and disaster monitoring and prediction small satellite constellation), vegetation investigation points, thawing layer thickness research sites, ground temperature and water content observation plots were used to examine the spatial pattern of vegetation which were located in different altitudes in Xidatan, a typical TP region, in Qinghai-Tibetan Plateau. Vegetation characteristics, soil moisture content(SMC) and thaw depths were collected in 15 August to 25 August2012. Characteristics of vegetation were mainly represented by fractional vegetation cover(FVC) derived from the normalized difference vegetation index(NDVI), as well as above ground biomass(AGB). In this paper, we analyzed that the distinction of vegetation characteristics in each range through statistics data. These ranges were divided by varied altitudes. For examples, the ranges were divided into 50 m or 100 m. In this study we use a large area plots method to further discuss the relationship between the features of vegetation and the different regions of permafrost based on altitudes shifts in Xidatan. A diagram described the vegetation characteristics variability with rising altitudes in transitional permafrost region was drawn in this paper. Our results illustrated the FVCs first increased in SFG region and then decreased in DCP zone slowly, and in CP region FVCs soared then dropped dramatically. With the altitudes increased, the curve of FVCs indicated a parabolic distribution except a little difference in the first 200 m range.     

Effect of Psammaplin A on in vitro Development of Bovine Aging Oocytes

To investigate the effect of histone deacetylation inhibitor Psammaplin A (PsA) on the development of bovine aging oocytes in vitro,oocytes were randomly divided into control group,aging group and 50 mmol/L PsA treated aging group (PsA group).Immunofluorescence staining and JC-1 were used to detect the blastocyst rate of bovine oocytes after parthenogenetic activation,the number of cells in blastocysts,apoptosis,reactive oxygen species (ROS),glutathione (GSH) and mitochondrial membrane potential intensity of embryos.The results showed that the blastocyst rate of the aging group was significantly lower than that of PsA and control groups (P<0.05).The blastocyst rate of PsA group was not significantly different from that of control group (P>0.05).The number of cells in the blastocysts of control group and PsA group were significantly higher than that of aging group (P<0.05).The number of cells in the blastocysts of PsA group was not significantly different from that of control group (P>0.05).The apoptosis rate in aging group was significantly higher than that of control and PsA groups (P<0.05),the apoptosis rate of PsA group was significantly higher than that of control group (P<0.05).The GSH level of MⅡ oocytes in aging group was significantly lower than that of control and PsA groups (P<0.05).There was no significant difference in GSH level between control and PsA groups (P>0.05).The ROS level of the embryos in aging group was significantly higher than that of control and PsA groups (P<0.05).The ROS level in PsA group was significantly higher than that of control group (P<0.05).The mitochondrial membrane potential of early embryos of aging group 4-8 cells was significantly lower than that of control and PsA groups (P<0.05).The mitochondrial membrane potential intensity of control group was significantly higher than that of PsA group (P<0.05).In summary,PsA could effectively delay the aging of bovine oocytes and improve the quality of oocytes.     

湘西北长果安息香种群数量与分布格局

对湖南石门壶瓶山景关门、桑植龙潭坪茅垭村样地的长果安息香(Changiostyrax raxdolichocarpa)种群数量和分布格局进行调查和分析。结果表明：长果安息香种群的年龄结构呈衰退型，个体数量主要集中在小树(IV龄段)，幼龄级株数和老龄级株数匮乏；长果安息香种群在进入生理死亡年龄前有2个死亡高峰，第1个峰值产生于幼树(II、III龄段)，主要原因是种内和种间竞争(主要是光照)，第2个峰值产生于中树长成大树阶段(V、VI龄段)，除种内、种间对环境的竞争外，生长地土层浅薄、大树倒伏及人为砍伐亦加剧了该峰值的产生；种群整体呈聚集分布类型，小树向中树、大树变化过程表现出聚集分布向随机分布转变，导致长果安息香濒危的主要原因是种子萌发率极低、种群竞争能力较差以及人为干扰等。