回交后代纯合体频率递增量的分布规律

回交后代纯合率是一单调递增函数，对每一个确定的杂合基因对数n，都存在一个最大的纯合体频率递增量△fmax及其对应的世代r，导数法和统计法都可确定△fmax所在的r，但用统计法推出的预测公式显得更便利且可靠，△fmax所在的r可用于确定停止回交的适宜世代，且在左侧前推2代，在右侧后延4代，一共7个世代，积累了95％以上的纯合体，这一现象在n≥5时，不因n的大小而异，表现出一致的规则性。     

金霉素和代谢物差向金霉素在肉鸡肌肉和肝脏中的残留分布

为了研究金霉素(CTC)、差向金霉素(ECTC)在肉鸡肌肉、肝脏中的残留分布情况,样品经提取液提取后过Oasis HLB SPE小柱净化,采用电喷雾正离子模式(ESI+)和多反应监测模式(MRM),用高效液相色谱-串联质谱仪检测;试验组鸡按体重以50 mg/(kg·d)剂量内服CTC,连续5 d,每天给药1次。结果表明:该方法测定肉鸡肌肉和肝脏中CTC检测限和定量限分别为5.0、10.0μg/kg。ECTC检测限和定量限分别为20.0、30.0μg/kg。CTC和ECTC在肉鸡肌肉中平均回收率分别在73.0%~95.20%和81.80%~101.60%,相对标准偏差(RSD)分别在8.85%~12.18%和2.05%~9.29%;在肉鸡肝脏中平均回收率分别在67.06%~90.80%和78.64%~106.98%,RSD分别在2.90%~8.88%和4.72%~9.48%;肉鸡肌肉和肝脏中CTC、代谢物ECTC的残留量分别在给药后第1、5 d时达到最高。肉鸡肝脏中ECTC的残留量约占总残留量的1/2。停药后CTC及其代谢物ECTC代谢缓慢,在停药第6 d时,仍能检测到CTC及其代谢物ECTC,但其残留量均低于最高残留限量(MRLs)。从保障食品安全方面考虑,建议我国将CTC和ECTC均作为金霉素的残留标示物进行监测。     

Review of African swine fever outbreaks history in South Africa: From 1926 to 2018

The article reviews the outbreaks and distribution of African swine fever (ASF) in South Africa since the first probable outbreak that occurred in the Koedoesrand Ward in 1926. Retrospective data on the ASF outbreaks in South Africa were obtained from the World Organisation for Animal Health (OIE) disease database and the South African veterinary services annual reports in addition to published articles and online sources. South Africa has experienced many outbreaks that can be divided into 2 time periods: the period before the development of the OIE diseases database (1993) and the period after. More than 141 outbreaks of ASF were reported during the first period. Since the development of OIE disease database, 72 outbreaks directly involving 2968 cases, 2187 dead and 2358 killed pigs mainly in smallholder pig farms were reported. The median number of cases for a given ASF outbreak is 17, but in 50% of outbreaks no pigs were killed for prevention. The most important ASF outbreak was reported in April 2014 in the Greater Zeerust district (North West province) involving 326 cases and 1462 killed pigs. However, the outbreak with highest mortality involving 250 pigs was reported in 2016 (Free State province). According to phylogenetic analysis, nine p72 genotypes (I, III, IV, VII, VIII, XIX, XX, XXI and XXII) have been identified in South Africa. Season-wise, more outbreaks were recorded during summer. It was also observed that the OIE disease database could contain errors that would have been introduced through compiled forms at country level. Spatiotemporal studies on ASF outbreaks in South Africa are therefore required in order to assess statistically and quantitatively the clustering of outbreaks over space and time.     

Tissue-specific expression of the NOD-like receptor protein 3 in BALB/c mice

Activation of the innate immune system requires recognition of pathogen-associated molecular patterns, such as NOD-like receptors. The NOD-like receptor protein 3 (NLRP3) inflammasome is involved in induction of the pro-inflammatory cytokine, IL-1β, and subsequent inflammatory responses. NLRP3 inflammasome plays important roles in the inflammatory and innate immune responses associated with autoimmune/inflammatory syndrome. However, analysis of the tissue distribution and expression profiles in BALB/c mice is still incomplete. In this study, we investigated the tissue distribution and expression pattern of NLRP3 in BALB/c mice to further elucidate its function in innate immunity in this commonly used laboratory animal model. NLRP3 mRNA expression levels and tissue distribution of the protein were investigated by real-time quantitative PCR and immunohistochemical analyses, respectively. NLRP3 mRNA expression was higher in the kidney and inguinal lymph nodes than in other tissues. Cytoplasmic expression of NLRP3 was detected in the epithelial reticular cells of the spleen and thymus, lymphocytes in the inguinal lymph nodes, cardiac muscle cells, cerebral cortex neurons, alveolar macrophages, renal tubule cells and liver sinusoidal endothelial cells. The results of this study will assist investigators in interpreting site-specific functions and roles of NLRP3 in inflammatory responses.     

A correction factor for,and its application to,visual censuses of littoral fish

Visual estimation techniques were used between October 1976 and May 1977 on the fish fauna of an enclosed mid-intertidal pool in the eastern Cape, South Africa, to test the usefulness and accuracy of this non-consumptive census method. In January, 1977, two pools were censused for fish, then poisoned using the ichthyocide ‘pronoxfish’ and all fish collected. Forty species in 21 families were obtained from the two stations. The accuracy of visual estimates varied with the species of fish. Secretive and cryptically coloured types, as well as species which Inhabit crevices, were underestimated, between 0 and 86% being counted. Other fish with protective colours but that occur more openly and do not react adversely to divers, as well as schooling species, were also underestimated, but up to 100% of the total were observed. Those which occur singly or in small groups in the water column were counted more accurately, with 57 to 100% of the actual number being seen. Correction factors were calculated from these data and applied to a census taken of one pool in May. Comparisons of corrected and actual numbers Indicate that these factors were relatively accurate for non-secretive species. The factors will, however, vary for each species, from observer to observer, depending on their personal experience, and from area to area.     

菜粉蝶绒茧蜂种群空间分布型及其应用

本文应用频次分布x~2检验法,Iwao法,Taylor幂法则以及其它聚集指标等方法,分析研究了菜粉蝶绒茧蜂种群(茧块)的空间分布型。结果表明:菜粉蝶绒茧蜂茧块在甘蓝上呈聚集分布,分布的基本成分是以个体群形式存在,个体群之间是随机的,茧块的聚集是由寄主等环境因素引起的。此外,利用分布型参数确定了理论抽样数及序贯抽样。     

中国风雪流的变化趋势和时空分布规律

利用1971-1996年中国194个站风雪流日数资料,分析中国风雪流发生日数的空间分布特征和时间变化趋势。结果表明,风雪流在全国影响区面积较大,42．8％的站发生过风雪流。主要集中在中国北方的东北、内蒙古、青海、甘肃、宁夏、新疆北部以及陕西、山西、山东、河南和河北的部分地区。风雪流最早出现在11月或12月,最晚终止在2～4月。风雪流发生日数的季节变化,有单峰值和双峰值两种类型。全国风雪流发生日数有减少趋势,但各地变化不同：东北地区、内蒙古、西藏的部分地区和新疆北部地区出现风雪流日数均呈下降趋势;青海、甘肃和宁夏部分地区发生风雪流的日数则呈上升趋势。从季节年变化趋势看,暖季的4～5月和9月风雪流出现日数有增加趋势,而冷季的10～3月风雪流出现日数则有下降趋势,且下降趋势强于上升趋势。     

Differential phytotoxicity of glyphosate in maize seedlings following applications to roots or shoot

The transport and differential phytotoxicity of glyphosate was investigated in maize seedlings following application of the herbicide to either roots or shoots. One-leaf maize seedlings (Zea mays L.) were maintained in graduated cylinders (250 mL) containing nutrient solution. Half of the test plants were placed in cylinders (100 mL) containing different ¹⁴C-glyphosate concentrations; the remainder received foliar appliation of ¹⁴C-glyphosate. After 26 h, the roots and the treated leaves were washed with distilled water, and the plants placed again in cylinders (250 mL) containing fresh nutrient solution for 5 days. Plants were weighed, and split into root, seed, cotyledon, coleoptile, mesocotyl, first leaf and apex. The recovery of ¹⁴C-glyphosate was over 86%. For both application treatments, the shoot apex was the major sink of the mobilized glyphosate (47.9 ± 2.93% for root absorption and 45.8 ± 2.91% for foliar absorption). Expressed on a tissue fresh weight basis, approximately 0.26 μg a.e. g⁻¹ of glyphosate in the apex produced a 50% reduction of plant fresh weight (ED₅₀) when the herbicide was applied to the root. However, the ED₅₀ following foliar absorption was only 0.042 μg a.e. g⁻¹ in the apex, thus maize seedlings were much more sensitive to foliar application of the herbicide.     

稻田蜘蛛混合种群空间分布的动态研究

稻田蜘蛛混合种群的空间分布在水稻的不同发育期存在差异。其空间分布主要由稻田蜘蛛本身的扩散程度及生殖 ,游猎型和定居型数量的多寡 ,主要目标害虫的数量及其分布 ,栖息环境的变化及其耕作方式和稻田蜘蛛的种群密度及其种间竞争程度决定。     

NaCl胁迫对苜蓿幼苗生长及不同器官中盐离子分布的影响

研究了不同浓度NaCl胁迫下(盐浓度处理为0,0.1%,0.3%,0.5%,0.7%,0.9%)苜蓿不同器官的生长情况(存活率、根长、茎长、叶片数),以及不同器官中Na⁺、K⁺、Ca²⁺、Mg²⁺的含量,并分析了K/Na、Ca/Na、Mg/Na值的变化。结果表明,存活率、根长、茎长与盐浓度呈负相关,且当NaCl≤0.1%时,有利于苜蓿的生长。除根茎叶中Na⁺含量、根中Ca²⁺与盐浓度呈正相关,茎叶中的Ca²⁺与盐浓度相关性不明显外,根茎叶中K⁺、Mg²⁺、K/Na、Ca/Na、Mg/Na均随盐浓度增加而显著下降,且盐处理与对照差异显著(P＜0.05)。另外从离子角度讨论了盐对苜蓿的毒害以及耐盐的原因。