插齿切削厚度计算机辅助计算与绘图

本文在文献[1] 的基础上,进一步研究了插齿刀切入刃、顶刃和切出刃的切削厚度,编制了计算机程序,并绘制了切削厚度曲线图,避免了繁杂的人工计算。     

Design of wireless inductive power receiver circuit in artificial subretinal chip

A scheme of inductive wireless receiver circuit is put forward, according to special requirement of inductive wireless power supply in artificial subretinal prosthesis circuit. The proposed receiver circuit can be divided into CMOS rectifier, regulator and self-biasing circuit. The CMOS rectifier is used to provide power supply for regulator and self-biasing circuit. The self-biasing circuit furnishes the regulator with bias voltage and bias current which is independent from power supply. The proposed receiver circuit is designed by Cadence tool and Chartered 0.35 μm CMOS device model, and oscillator in artificial subretinal chip is used as the proposed receiver circuit’s load. The results show that the proposed receiver circuit can provide load capacity of 3.3 V and >1 mA,with a layout size of 62 μm×195 μm.     

利用基因芯片技术筛选棉花产量性状相关的基因

根据皮棉产量数据的重复性测验及显著性分析,从SG747(Gossyp ium hirsutum L.)和Giza75(Gossypium Barbadense L.)的回交自交系群体中选取高产组和低产组品系各3个材料,取这2组材料和其父母本开花后10d的纤维进行芯片分析.比较芯片数据,获得了1508个差异表达基因.对这些差异表达基因进行聚类分析,结果显示高产组品系和低产组品系分别聚类在一起,符合基于田间数据分组的预期结果.利用Blast2GO软件对1508个差异表达基因进行Blast-Mapping-Annotation-KEGG分析,结果表明参与细胞质膜发育、过氧化物酶活性、抗逆和营养物质运输等生物学过程的基因最多.进一步利用RT-PCR分析,得到一系列与纤维品质和产量等性状密切相关的基因.研究结果为棉花高产基因工程和分子育种提供了丰富的基因资源.     

Genomewide association for a dominant pigmentation gene in sheep

Most published genomewide association studies (GWAS) in sheep have investigated recessively inherited monogenic traits. The objective here was to assess the feasibility of performing GWAS for a dominant trait for which the genetic basis was already known. A total of 42 Manchega and Rasa Aragonesa sheep that segregate solid black or white coat pigmentation were genotyped using the SNP50 BeadChip. Previous analysis in Manchegas demonstrated a complete association between the pigmentation trait and alleles of the MC1R gene, setting an a priori expectation for GWAS. Multiple methods were used to identify and quantify the strength of population substructure between black and white animals, before allelic association testing was performed for 49 034 SNPs. Following correction for substructure, GWAS identified the most strongly associated SNP (s26449) was also the closest to the MC1R gene. The finding was strongly supported by the permutation tree‐based random forest (RF) analysis. Importantly, GWAS identified unlinked SNP with only slightly lower p‐values than for s26449. Random forest analysis indicated these were false positives, suggesting interpretation based on both approaches was beneficial. The results indicate that a combined analytical approach can be successful in studies where a modest number of animals are available and substantial population stratification exists.     

Patent analysis provides insights into the history of cotton molecular breeding worldwide over the last 50 years

Cotton is a globally important natural fiber and oilseed crop of crucial economic significance. Molecular breeding has become a dominant method of cotton cultivation because it allows for a shorter breeding period and directional selection of high quality genes. Patent data are key resources and are the core competitiveness of agricultural development, as the world's largest and most reliable source of technical information. However, little attention has been paid to patent analysis of cotton molecular breeding. This study uses bibliometric analysis methodology and technical classification indexing to reveal global development trends of cotton molecular breeding, based on patents by retrieval methods and expert screening. The annual number of patents, the life-cycle of patent-based technology, patent portfolios of primary countries, and main patentees, as well as technical distribution of patents, were analyzed in this study. In addition, this study put emphasis on the comparative analysis of two important patentees through patent roadmaps based on the relationship among patent citations. Finally, in order to understand the trend of new molecular breeding technology, patents related to clustered regularly interspaced short palindromic repeats (CRISPR), RNA interference (RNAi), and gene chip were also analyzed, all of which apply to cotton but also to other crops. Results in this paper can provide references for cotton molecular breeding researchers and relevant management departments.     

基于AD7705的溶解氧检测C51程序设计

介绍了高精度16位模,数转换器AD7705在一种溶解氧检测仪中的应用,AD7705同单片机的接口、内部寄存器结构及其配置和使用方法,并给出了在C51语言基础上的设计与编程,并附C源程序,有较强的参考性。     

微流控芯片在农产品安全检测中的应用

近年来,随着食品工业的迅猛发展,国内外食品安全事故也频繁发生。开发安全可靠、便携有效的检测方法对食品安全至关重要。微流控芯片集样品前处理、分离和检测等过程为一体,实现了样品前处理和检测分析的自动化、集成化、小型化、低消耗、高效率和便携化,满足了现场、实时检测的需求。本研究对微流控芯片在食品成分、农药残留、兽药残留、食品添加剂和重金属检测中的应用进行归纳总结,为农产品安全检测提供参考。     

Genetic Diversity Analysis of Microsatellite DNA Markers in Swamp Buffalo

This study was aimed to investigate the genetic diversity of swamp buffalo breeds in China.The analysis of genetic diversity was performed in 40 buffalo individuals from 8 buffalo breeds（Dechang buffalo,Dehong buffalo,Wenzhou buffalo,Guizhou buffalo,Xilin buffalo,Fuzhong buffalo,Murrah buffalo and Nili-Ravi buffalo）by 30 microsatellite loci and LabChip chip test method.The results showed that 332 alleles at 30 microsatellite loci were found in 8 buffalo breeds,the average values of gene diversity and PIC were 0.7808 and 0.7554,respectively.Cluster analysis indicated that Dechang buffalo and Dehong buffalo firstly clustered together,followed by Fuzhong buffalo,Guizhou buffalo,Wenzhou buffalo and Xilin buffalo.Moreover,Murrah buffalo and Nili-Ravi buffalo clustered together.Our findings revealed that the 30 microsatellite loci could be used as an effective genetic marker for the analysis of genetic diversity among the buffalo breeds,which enriched the current SSR marker resources in buffalo.     

不同茭白品种福寿螺卵块分布及竹片诱螺产卵效果

为了研究控制茭白田福寿螺种群的方法,本文在调查不同茭白品种上福寿螺卵块分布差异基础上,开展不同规格(3、6、9 cm宽)竹片诱集福寿螺产卵试验。结果显示,不同茭白品种上福寿螺卵块分布数量差异大,单季茭白品种以‘杭州一点红’上卵块最多(11.6个/丛),远高于其他9个品种,最少的是‘八月茭’‘金茭1号’(分别为1.7、2.3个/丛)。双季茭白品种间的差异不显著,每个茭白品种上的卵块数在5.1~7.4个之间。不同规格竹片对福寿螺的产卵诱集效果差异大,引诱效果最好的为9 cm规格的竹片效果最差的是3 cm规格竹片。竹片凸面诱集到的卵块较多,凹面诱集到的卵块很少,竹片凸面朝田埂方向插入诱集产卵效果更好。福寿螺的产卵高度呈正态分布,主要集中在20~40 cm之间,未发现有靠近水面或在水下的卵块,卵块大小与数量呈线性正相关。研究结果为利用竹片诱集茭白田福寿螺产卵提供了技术依据。     

全基因组基因表达分析技术及其在果树上的应用

基因表达分析对于揭示基因功能、了解基因间相互关系、阐明植物生长发育过程中的生理生化调控机制起着至关重要的作用。综述了mRNA差异显示、cDNA-AFLP、基因芯片、EST数据分析、基因表达系列分析(SAGE)等全基因组基因表达分析技术的原理特点及在果树上的应用情况,并介绍了利用全基因组基因表达分析技术开展果树研究的思路。