Sertoli cell tumors associated with feminizing syndrome and spermatic cord torsion in two cryptorchid dogs

The association of cryptorchidism, functional Sertoli cell tumors, and spermatic cord torsion has been rarely reported in the literature. Two dogs were admitted for bilateral skin alopecia and weight loss. Both animals were cryptorchid and displayed a pendulous preputial sheath, prostate hypertrophy, and increased levels of circulating oestrogen. Transabdominal palpation and ultrasonography revealed the presence of neoplastic retained gonads. During surgery, spermatic cord torsion was also detected in the enlarged neoplastic testes of both dogs. Histologic examination confirmed the presence of Sertoli cell tumors that were primarily responsible for the feminizing syndrome. Complete remission of all symptoms occurred within 3 months after orchiectomy.     

曲线连续箱梁桥支座设置的比较

以实际工程为例,对采用MIDAS空间梁单元小半径曲线梁桥支座的布置进行计算和比较分析,并通过掌握小半径曲线梁桥支座受力特点,从而确定出适合该桥的支座布置形式。     

IMAGING DIAGNOSIS—PULMONARY ALVEOLAR PROTEINOSIS IN A DOG

A young dog was presented for cyanosis and right heart failure. Radiographic and CT characteristics included right heart/pulmonary artery enlargement, hepatomegaly, abdominal effusion, and severe, generalized air‐space filling. Focal increased opacities were present in the peripheral lung, as were multiple pulmonary blebs and bullae. Echocardiographic findings were consistent with cor pulmonale and pulmonary hypertension. Bronchoscopic findings were consistent with chronic inflammation. Pulmonary alveolar proteinosis (PAP) was confirmed at necropsy. Pulmonary alveolar proteinosis is an interstitial lung disease that results in accumulation of phospholipoproteinaceous material and should be included as a differential diagnosis for dogs with these clinical and imaging characteristics.     

Surgical Correction of Uterine Torsion and Mare–Foal Survival in Advance Pregnant Equine Patients

This article describes the surgical management of uterine torsion by midline celiotomy and cesarean section on 12 mares presented with signs of colic to a teaching veterinary hospital. The mares were either in full term of gestation (n = 7) or in advanced stage of pregnancy (n = 5). Six mares were in first parity. Uterine torsion was diagnosed by per rectal and per vaginal examinations. For surgical intervention, mares were anesthetized using a combination of xylazine (1.1 mg/kg) and ketamine (2.2 mg/kg), intravenously. After intubation, the animals were maintained on halothane (n = 4) or isoflurane (n = 8) inhalation anesthesia. Midline celiotomy was performed, and foals were delivered by cesarean section. In 11 mares, before closing the abdominal wound, the uterus was detorted manually and confirmed for its normal position. Both anesthetic protocols using halothane and isoflurane were found satisfactory for surgical correction of uterine torsion. After long-term follow-up, the study reported 75.0% (9/12) survival rate for mares. One mare was euthanized because of devitalized, necrosed, and adhered uterus to the abdominal wall. Of the nine surviving mares, seven were successfully bred. Three foals were born alive, and only one could survive on long-term basis. Of the nine dead foals, two had umbilical cord torsion.     

基于数据挖掘技术探讨中医治疗肺癌放疗患者的用药规律

目的 探讨中医治疗肺癌放疗患者的用药规律。方法 检索中国知网数据库（CNKI）、万方数据库、维普数据库（VIP）、中国生物医学文献数据库（CBM）、PubMed数据库公开发表的治疗肺癌放疗患者的中药处方,运用频次分析、聚类分析、关联规则分析方法对收集的处方进行数据挖掘,分析其用药规律。结果 共收集到处方70张,涉及176味中药,使用频次共计890次;使用频次≥9的药物共32味;使用频次前三位的单味中药是黄芪、甘草、麦冬;使用频次前三位的药类是补虚药、清热药、化痰止咳平喘药;对32味药物进行聚类分析和关联规则分析,得出2个有效聚类群和13组药对,以补气药和滋阴药配伍为主,辅以补血活血、化痰止咳药。结论 治疗肺癌放疗患者的中药处方以补益药、清热药和止咳药为主,治法以益气养阴、清肺止咳为主。     

microRNA-124-3p调控H1N1亚型猪流感病毒致小鼠肺损伤的研究

为研究microRNA-124-3p（miR-124-3p）对H1N1亚型猪流感病毒（swine influenza virus，SIV）感染小鼠所致肺损伤的调控作用，本试验构建miR-124-3p腺病毒表达载体，通过小鼠尾部静脉注射法构建miR-124-3p差异表达小鼠模型，试验分3组：过表达组、抑制组和对照组。48 h后，各组小鼠鼻腔接种H1N1亚型SIV，每只10⁵ EID₅₀（50 μL）。连续观察14 d，计算小鼠平均体重变化率、观察病理切片并测定相关炎症因子IL-1β、TNF-α和IL-6 mRNA相对表达量。结果显示，已成功将pre-miR序列及其sponge序列插入腺病毒的穿梭质粒，并将其共转染293A细胞。实时荧光定量PCR检测证实，与对照组相比，过表达组和抑制组小鼠黑色素瘤细胞miR-124-3p表达水平分别极显著升高（P<0.01）和显著降低（P<0.05），表明成功构建腺病毒表达载体。过表达组、抑制组和对照组小鼠体重变化率分别为－5.5%、－12.4%和－8.6%。抑制组和对照组均可见肺泡壁增厚，其间有多量淋巴细胞浸润，部分肺泡内出现纤维蛋白渗出，且抑制组病理变化更为严重，肺泡中还有大量的红细胞浸润；而过表达组仅有少量的淋巴细胞浸润，肺脏组织较正常。与对照组相比，过表达组检测的炎症因子IL-1β、TNF-α和IL-6 mRNA表达水平均显著降低（P<0.05）；抑制组炎症相关炎症因子mRNA表达水平均显著升高（P<0.05）。本试验结果表明，miR-124-3p对H1N1亚型SIV感染小鼠所致的肺脏炎症因子的表达具有抑制作用，同时能减轻肺脏病理损伤。     

Knockdown of HMGA2 gene inhibits TGF-β1-induced human embryonic lung fibroblast growth and collagen synthesis

AIM: To investigate the effects of high mobility group A2(HMGA2) gene knockdown on the cell viability, apoptosis, collagen synthesis and oxidative stress of human embryonic lung fibroblast (HELF) induced by transforming growth factor-β1 (TGF-β1). METHODS: The HELF were divided into blank group, TGF-β1 group,negative control (NC) group and HMGA2 siRNA(si-HMGA2) group. The protein levels of HMGA2, AKT and p-AKT were determined by Western blot. The cell viability and apoptotic rate was analyzed by MTT assay and flow cytometry,respectively. The mRNA expression of collagen I (COL-Ⅰ) and COL-Ⅲ was detected by RT-qPCR. DCFH-DA was used to detect the content of reactive oxygen species (ROS). RESULTS: Compared with blank group, the protein levels of HMGA2 and p-AKT, the cell viability, the mRNA expression of COL-Ⅰ and COL-Ⅲ in TGF-β1 group were significantly increased, but the apoptotic rate and ROS level were significantly decreased (P<0.05). Compared with TGF-β1 group, the protein levels of HMGA2 and p-AKT, the cell viability, the mRNA expression of COL-Ⅰ and COL-Ⅲ in si-HMGA2 group were significantly decreased, but the apoptotic rate and ROS level were significantly increased (P<0.05). CONCLUSION: Knockdown of HMGA2 gene expression decreases the viability and collagen synthesis, and promotes apoptosis and ROS production of human embryonic lung fibroblasts induced by TGF-β1. The mechanism may be related to down-regulation of PI3K/AKT signaling pathway.     

Right cranial lung lobe torsion after a diaphragmatic rupture repair in a Jack Russell terrier

A seven-year-old male Jack Russell terrier was presented with a history of coughing, generalised weakness and lethargy 10 days after an abdominal coeliotomy to repair a large diaphragmatic rupture. Thoracic radiographs demonstrated a soft tissue mass in the midcaudal right thoracic cavity. Ultrasonographic studies, bronchoscopy and subsequent exploratory thoracotomy confirmed a diagnosis of a right cranial lung lobe torsion (LLT), with an anomalous caudodorsal displacement of the affected lobe. LLT should be considered as a differential diagnosis for respiratory tract disease following diaphragmatic rupture repair.     

Effects of 12 and 17 cmH2O positive end-expiratory pressure applied after alveolar recruitment maneuver on pulmonary gas exchange and compliance in isoflurane-anesthetized horses

Objective

To compare static compliance (C_st) and alveolar–arterial oxygen tension difference [P(a–a)O₂] between positive end-expiratory pressures (PEEP) of 7, 12 and 17 cmH₂O applied after an alveolar recruitment maneuver (RM) in isoflurane-anesthetized horses.