全文获取类型
收费全文 | 11953篇 |
免费 | 500篇 |
国内免费 | 1518篇 |
专业分类
林业 | 728篇 |
农学 | 1332篇 |
基础科学 | 682篇 |
1576篇 | |
综合类 | 5182篇 |
农作物 | 838篇 |
水产渔业 | 576篇 |
畜牧兽医 | 1637篇 |
园艺 | 757篇 |
植物保护 | 663篇 |
出版年
2024年 | 54篇 |
2023年 | 152篇 |
2022年 | 316篇 |
2021年 | 365篇 |
2020年 | 428篇 |
2019年 | 560篇 |
2018年 | 465篇 |
2017年 | 562篇 |
2016年 | 664篇 |
2015年 | 548篇 |
2014年 | 596篇 |
2013年 | 654篇 |
2012年 | 757篇 |
2011年 | 758篇 |
2010年 | 677篇 |
2009年 | 658篇 |
2008年 | 577篇 |
2007年 | 559篇 |
2006年 | 479篇 |
2005年 | 389篇 |
2004年 | 318篇 |
2003年 | 262篇 |
2002年 | 237篇 |
2001年 | 204篇 |
2000年 | 213篇 |
1999年 | 185篇 |
1998年 | 171篇 |
1997年 | 252篇 |
1996年 | 242篇 |
1995年 | 207篇 |
1994年 | 237篇 |
1993年 | 226篇 |
1992年 | 270篇 |
1991年 | 266篇 |
1990年 | 226篇 |
1989年 | 93篇 |
1988年 | 43篇 |
1987年 | 27篇 |
1986年 | 14篇 |
1985年 | 8篇 |
1984年 | 13篇 |
1982年 | 5篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1977年 | 2篇 |
1956年 | 6篇 |
1955年 | 6篇 |
1944年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
21.
【目的】研究头孢氨苄片受试制剂和参比制剂在比格犬体内的生物等效性。【方法】采用双周期和双序列交叉设计,将22只健康比格犬随机分成2组,按30 mg/kg BW分别单剂量口服头孢氨苄片受试制剂Trolevis~?300和参比制剂Rilexine~?300,于给药前(0 h)和给药后0.5、1、1.5、2、2.5、3、4、6、8、12、17和24 h从臂头静脉采血。对超高效液相色谱串联质谱(UPLC-MS/MS)方法进行特异性、线性、检测限、准确度、精密度、稳定性等方法学考察。利用建立好的UPLC-MS/MS方法测定血浆中的药物浓度,并用WinNonlinTM 8.1软件对药代动力学参数进行分析计算。【结果】方法学结果显示,在100~5 000 ng/mL浓度范围内相关性良好,相关系数(R2)≥0.99,标准曲线方程为y=10.6828x-176.481;高、中、低3个浓度的相对回收率平均值分别为105.63%、104.35%和102.40%;日内和日间变异系数均<15%;检测限为50 ng/mL,定量限为100 ng/mL。药代动力学结果... 相似文献
22.
23.
HUANG Yi HU Jian-da ZHENG Jing LI Jing WEI Tian-nan ZHENG Zhi-hong CHEN Ying-yu 《园艺学报》2012,(1):70-75
AIM: To study the effects of baicalin on CA46 cell xenografts in nude mice. METHODS: The nude mice with CA46 cell xenografts were treated with drugs via intraperitoneal injection daily, and were divided into 5 groups: negative control group, 15 mg/kg baicalin group, 30 mg/kg baicalin group, 60 mg/kg baicalin group and 4 mg/kg etoposide (VP-16) positive control group. After 12-day treatment, the weight of CA46 cell xenografts stripped from some nude mice in the 5 groups was used to evaluate the effect of baicalin on xenograft growth in the nude mice. The apoptosis, necrosis and pathological changes of the xenograft cells were examined under light microscope and transmission electronic microscope respectively. The expression levels of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway-related proteins extracted from xenografts were determined by Western blotting. The other nude mice with CA46 cell xenografts in the 5 groups continued to be treated with the drugs until death in order to evaluate the effect of balcalin on survival time of the nude mice with CA46 cell xenografts. RESULTS: Baicalin remarkably inhibited the growth of CA46 cell xenografts, induced apoptosis and necrosis of xenograft cells, and reduced the protein expression of phospho-Akt (p-Akt), nuclear factor-kappa B (NF-κB), mammalian target of rapamycin (mTOR) and phospho-mTOR (p-mTOR) in the xenografts after 12-day treatment. Furthermore, baicalin prolonged the survival time of the nude mice with CA46 cell xenografts in a dose-dependent manner. CONCLUSION: Baicalin inhibits the growth and induces apoptosis of CA46 cell xenografts in the nude mice, and prolongs the survival time of the nude mice with CA46 cell xenografts through the mechanism of down-regulating PI3K/Akt/NF-κB and PI3K/Akt/ mTOR signaling pathways. 相似文献
24.
采用不同比例丹参(Salviae miltiorrhiza)药渣替代玉米芯对糙皮侧耳(Pleurotus ostreatus)、毛头鬼伞(Coprinus comatus)供试菌株进行栽培实验.经栽培收获两茬子实体,分别对不同配方两个菌种的菌丝长速、单位料重产量、生物学效率等指标进行统计分析.结果表明:在单位料重产量及生物学效率表现上,随培养料中所含丹参药渣比例增加而增加;在菌丝长速表现上则相反.经丹参药渣、玉米芯的碳氮比分析(28.5∶1;88.1∶1)得知,栽培料中随药渣含量的增加氮素含量相应提高(碳氮比55.27∶1~27.44∶1,氮含量为0.76%~2.43%),成为两个菌种产量增加的主要原因. 相似文献
25.
超高效液相色谱-串联质谱法检测棉花和土壤中呋虫胺残留 总被引:1,自引:0,他引:1
在QuEChERS方法基础上,建立了棉花和土壤中呋虫胺残留的超高效液相色谱-串联质谱(UPLC-MS/MS)快速检测分析方法。方法选用乙腈为提取剂,N-丙基乙二胺(PSA)和石墨化炭黑(GCB)为净化剂,外标法定量。添加回收试验结果表明,不同添加浓度的呋虫胺(0.01、0.05和0.5mg/kg)在棉花植株、棉籽和土壤中的平均回收率为80.9%~107.5%,变异系数为3.3%~10.3%,定量限(LOQ)分别为4.81、3.41和2.26μg/kg。呋虫胺在棉花植株上的消解动态表明,呋虫胺在河南省和山东省两地棉花植株中的降解半衰期分别为1.9d和1.2d。 相似文献
26.
27.
NDeye Lallah Nina Koite Ngouro Issa Sanogo Olivier Lpine Jean-Marie Bard Khadija Ouguerram 《Marine drugs》2022,20(7)
Lipid peroxidation is associated with the development of some pathologies, such as cardiovascular diseases. Reduction in oxidative stress by antioxidants, such as Arthrospira (formely Spirulina), helps improving this redox imbalance. The aim of the study was to evaluate the effect of the Arthrospira liquid extract “Spirulysat®” on oxidative markers—in particular, oxidized LDL (oxLDL)/total LDL cholesterol—and isoprostanes and to investigate its impact on lipid and glucose metabolism in the metabolic syndrome subject. A controlled, randomised, double-blind design was conducted in 40 subjects aged 18 to 65 years with metabolic syndrome after a daily intake of Spirulysat® or placebo for twelve weeks. Blood and urinary samples were collected at three visits (V1, V2, V3) in the two groups for parameters determination. Although the Spirulysat® group showed a decrease at all visits of the oxLDL/total cholesterol ratio, there was no significant difference compared to the placebo (p = 0.36). The urinary isoprostanes concentration in the Spirulysat® group was reduced (p = 0.014) at V3. Plasma triglycerides decreased at V3 (p = 0.003) and HDL-cholesterol increased (p = 0.031) at all visits with Spirulysat®. In conclusion, Spirulysat® did not change the oxidized LDL (oxLDL)/LDL ratio but decreased the urinary isoprostanes, plasma triglycerides and increased HDL cholesterol, suggesting a beneficial effect on metabolic syndrome. 相似文献
28.
Sergey A. Dyshlovoy Sergey N. Fedorov Vasily I. Svetashev Tatiana N. Makarieva Anatoliy I. Kalinovsky Olga P. Moiseenko Vladimir B. Krasokhin Larisa K. Shubina Alla G. Guzii Gunhild von Amsberg Valentin A. Stonik 《Marine drugs》2022,20(7)
The cytotoxicity-bioassay-guided fractionation of the ethanol extract from the marine sponge Guitarra abbotti, whose 1-O-alkyl-sn-glycerol ethers (AGEs) have not been investigated so far, led to the isolation of a complex lipid fraction containing, along with previously known compounds, six new lipids of the AGE type. The composition of the AGE fraction as well as the structures of 6 new and 22 previously known compounds were established using 1H and 13C NMR, GC/MS, and chemical conversion methods. The new AGEs were identified as: 1-O-(Z-docos-15-enyl)-sn-glycerol (1), 1-O-(Z-docos-17-enyl)-sn-glycerol (2), 1-O-(Z-tricos-15-enyl)-sn-glycerol (3), 1-O-(Z-tricos-16-enyl)-sn-glycerol (4), 1-O-(Z-tricos-17-enyl)-sn-glycerol (5), and 1-O-(Z-tetracos-15-enyl)-sn-glycerol (6). The isolated AGEs show weak cytotoxic activity in THP-1, HL-60, HeLa, DLD-1, SNU C4, SK-MEL-28, and MDA-MB-231 human cancer cells. A further cytotoxicity analysis in JB6 P+ Cl41 cells bearing mutated MAP kinase genes revealed that ERK2 and JNK1 play a cytoprotective role in the cellular response to the AGE-induced cytotoxic effects. 相似文献
29.
30.
为了建立适于基层单位应用的针对副溶血性弧菌检测的特异PCR方法,检测副溶血性弧菌的基因序列.针对该菌的属特异性基因t1基因进行引物设计,扩增片段大小为449 bp.运用该PCR法可特异性的从副溶血性弧菌ATCC17802标准株中扩增出目的基因片段,与GenBank上发表的序列同源性为100%.而经常污染海产品的溶藻弧菌,嗜水气单胞茵标准株J-1,铜绿假单胞茵标准株ATCC27853结果均为阴性.该PCR法最低检出菌体DNA量为10-2ng以及最低检出菌数为5.7 × 103 CFU/mL.对临床上送检的35份样品进行菌体DNA PCR方法检测并同时与国标GB/T4789.7-2003中使用的检测方法进行比对,两种检测方法的结果完全符合,与国标中使用的检测方法相比可大大缩短鉴定时间.因此该PCR法能快速鉴定当前副溶血性弧菌流行群,有利于流行病学溯源等研究. 相似文献