二氢吡啶(diludin)抗蛋鸡脂肪肝的机理

30周龄罗曼蛋鸡 80 0只 ,随机分为 2组 (每组设 4个重复 ,每个重复 10 0只 ) ,分别饲喂添加二氢吡啶 (diludin) 0、15 0 m g/ kg的玉米豆粕日粮 ,分别于试验 2 0周和 40周采血和屠宰 ,研究了二氢吡啶抗蛋鸡脂肪肝的机制。结果显示 ,日粮中添加二氢吡啶 ,试验鸡于 5 0、70周龄时 ,肝脂率和腹脂率均显著降低 (P<0 .0 5或 P<0 .0 1) ;腹脂激素敏感脂酶 (HSL )活性显著提高 (P<0 .0 5或 P<0 .0 1) ,脂蛋白脂酶 (L PL )活性显著降低 (P<0 .0 5或 P<0 .0 1) ,肝脏中NADPH生成酶 - MDH活性显著降低 (P<0 .0 1) ;血清脂肪酶活性和游离脂肪酸、极低密度脂蛋白胆固醇含量显著提高 (P<0 .0 5或 P<0 .0 1) ;血清甘油三酯含量降低 (P<0 .0 5或 P<0 .0 1) ;血清超氧化物歧化酶和 T3 显著提高 (P<0 .0 5或 P<0 .0 1) ;肝脏、腹脂中环腺苷酸 (c AMP)含量显著提高 (P<0 .0 5 )。上述结果表明 ,二氢吡啶通过促进产蛋中后期蛋鸡肝脏中脂肪的迁移和体脂的分解 ,达到抗脂肪肝效果。     

miR-144表达质粒的构建及其对肝癌细胞生物学行为的影响

【目的】构建microRNA-144(miR-144)的真核表达质粒,并探讨miR-144对人肝癌细胞株HepG2生物学行为的影响,为肝癌的生物学治疗提供依据。【方法】构建miR-144真核表达质粒并合成miR-144反义寡核苷酸,通过实时定量PCR检测miR-144的表达,采用MTT比色法及流式细胞仪技术,检测抑制与过表达miR-144对肝癌细胞增殖、凋亡和细胞周期的影响。【结果】成功构建了miR-144的真核表达质粒,过表达miR-144能够显著抑制肝癌细胞的增殖(P<0.05),促进细胞凋亡(P<0.05);而抑制miR-144的表达则得到相反的结果;抑制与过表达miR-144对肝癌细胞周期的影响都不大(P>0.05)。【结论】miR-144能影响人肝癌细胞株HepG2的生物学行为,具有抑制肝癌细胞增殖的作用。     

The Distribution of Soluble Copper- and Zinc-Binding Proteins in Liver and Kidney of Chronic Copper-Poisoned Goats

The distribution of copper and zinc among the soluble proteins in the liver and kidney from chronic copper-poisoned goats was examined after gel filtration of the proteins. The concentrations of copper in the liver and kidney cortex from five experimentally copper-poisoned goats were: 550–810 µg/g liver and 190–420 µg/g kidney cortex (wet weight). In general the copper-binding proteins from, both the liver and kidney samples were separated into two different fractions with approximate molecular weights (m.w.) of > 65,000 and 10,000, respectively. From the liver samples, varying amounts of copper were eluted in a fourth fraction with m.w. < 2,000. In the majority of kidney samples the dominating copper-binding protein fraction was the high molecular weight fraction. Absolute amounts of copper recovered in the metallothionein-like protein fraction were nearly the samt for all samples investigated. The distribution of zinc-binding proteins in both liver and kidney samples was nearly the same. The high molecular weight fraction dominated, and no zinc was bound to metallothionein-like proteins.     

Hepatic abscesses in cats: 14 cases (1985-2002)

In this retrospective study, we describe 14 cats diagnosed with hepatic abscesses. The objective of the study was to report the clinical signs, physical examination findings, clinicopathologic findings, and outcomes in affected cats. These findings were then compared with those previously reported in dogs and humans. Clinical signs were vague and included anorexia, lethargy, and weight loss. Only 23% of cats had fever, whereas 31% were hypothermic. Increases in serum activities of alanine aminotransferase and alkaline phosphatase were found in 45 and 18%, respectively, of the 11 cats that had laboratory work performed. Abdominal ultrasound examinations were performed in 7 cats, and abnormalities were found in 71% of them. Four cats had solitary abscesses, all of which were located in the right liver lobes. The other 10 cats had multifocal small abscesses or microabscesses, and all of these cats had clinical signs suggestive of sepsis. Cytologic evaluation of samples obtained by abdominocentesis indicated septic inflammation in 67% of cats in which peritoneal fluid was analyzed. Hepatic abscess cultures yielded polymicrobial growth in 66% of the cats: Escherichia coli was the most commonly cultured organism. Overall mortality rate was 79%. All survivors underwent exploratory laparotomy for partial hepatectomy to resect the abscess followed by medical management. Hepatic abscesses should be considered in cats with signs consistent with sepsis. More routine use of ultrasonography may aid in earlier diagnosis of hepatic abscesses, potentially improving prognosis and outcome.     

豚鼠至大鼠原位肝移植肝下下腔静脉套管方法的改进

目的探讨豚鼠至大鼠异种原位肝移植中肝下下腔静脉套管的改进方法。方法豚鼠和SD大鼠各40只分别作为供、受体,随机分为实验组和对照组并随机配对。实验组采用改进的肝下下静脉套管方法进行肝下下腔静脉袖套管的安装及吻合,对照组采用常规方法进行肝下下腔静脉袖套管的安装及吻合。比较两组肝下下腔静脉袖套管安装时间、安装成功率、吻合时间、吻合成功率和手术成功率。结果实验组较对照组肝下下腔静脉袖套管安装时间、吻合时间和无肝期明显缩短,袖套安装成功率、吻合成功率和手术成功率明显提高,差异有统计学意义(P<0.05)。结论应用改进的肝下下腔静脉套管方法进行豚鼠至大鼠原位肝移植,手术成功率高,可用于豚鼠至大鼠原位肝移植实验研究模型。     

Study on the Hepatoprotective Effect of Oxalis coriniculata L. and Related Mechanism by Regulating Oxidative Stress and TLR-2/NF-κB Signaling Pathway

[Objectives]This study aimed to explore the protective effect of Oxalis coriniculata L.on rats with acute liver injury induced by carbon tetrachloride(CCl4)and ...     

镰刀菌毒素对断奶仔猪血清酶、肝脏抗氧化功能及组织病理学的影响

试验旨在研究镰刀菌毒素污染日粮对断奶仔猪血清酶、肝脏抗氧化功能及组织病理学的影响。选择35日龄体重为(8.45±0.94) kg的健康三元杂交(杜×长×大)断奶雌性仔猪40头,随机分为2组,每组20个重复,每个重复1头猪。对照组饲喂基础日粮,试验组饲喂用霉变玉米和霉变玉米蛋白粉替代基础日粮中50%的玉米和玉米蛋白粉配制而成的镰刀菌毒素污染日粮(玉米赤霉烯酮(ZEN) 0.90 mg/kg,呕吐毒素(DON) 1.43 mg/kg,烟曲霉毒素(FUM) 5.85 mg/kg)。仔猪单栏饲喂,预试期7 d,正试期35 d。结果表明:①与对照组相比,试验组断奶仔猪血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)和碱性磷酸酶(ALP)活性均显著升高(P<0.05),肝脏相对重显著降低(P<0.05);②与对照组相比,试验组断奶仔猪血清和肝脏中谷胱甘肽过氧化物酶(GSH-Px)和总超氧化物歧化酶(T-SOD)活性均显著降低(P<0.05),而丙二醛(MDA)含量显著升高(P<0.05);③病理组织学观察发现,试验组断奶仔猪肝脏出现一定程度的组织损伤,部分肝小叶边缘发生明显的炎性细胞浸润,肝细胞发生局部空泡变性。由此可知,镰刀菌毒素污染日粮(0.90 mg/kg ZEN,1.43 mg/kg DON,5.85 mg/kg FUM)饲喂断奶仔猪可引起仔猪血清酶活性改变,肝脏抗氧化功能降低,肝脏组织出现一定程度的病理损伤,从而影响肝脏功能的发挥。     

Healing and Preventive Effects of Calcium Alginate on Carbon Tetrachloride Induced Liver Injury in Rats

The purpose of this study was to investigate the pharmacological effects of calcium alginate on carbon tetrachloride (CCL₄)-induced hepatotoxicity in rats. The study included two experiments. In the first experiment the animals were given daily CCL₄ through gavage for 7 days and then 10, 50, or 250 mg/kg b.w. of calcium alginate for 21 days. The increased bilirubin level, enhanced alanine and aspartate aminotransferase activity in plasma and reduced liver glycogen content induced by CCL₄ were partly normalized by alginate administration in a dose-dependent manner. In addition, alginate significantly improved CCL₄-induced alterations of pro-oxidant and antioxidant biochemical parameters in liver and plasma compared to those of rats administered CCL₄. In the second experiment the animals were given daily 10, 50 or 250 mg/kg b.w. of calcium alginate for 21 days before 7-day administration of CCL₄. Pretreatment with alginate before CCL₄ administration resulted in significantly inhibited increase of the blood enzymatic activities of alanine and aspartate aminotransferases and bilirubin level in a dose-dependent manner. Also, preliminary administration of alginate prevented elevation of lipid peroxidation products and reduction of liver glutathione content in rats given CCL_4. These results suggest that calcium alginate exerts healing and preventive effects on CCL₄-induced hepatotoxicity in rats.     

Studies on the green liver in cultured red sea bream fed low level and non-fish meal diets: Relationship between hepatic taurine and biliverdin levels

SUMMARY: The aim of this study was to investigate the cause of green liver in red sea bream fed substitute protein diets. Red sea bream Pagrus major was given either of the following diets for 28 weeks: (1) control diet (50% fish meal), (2) low level fish meal diet (15% fish meal), and (3) non-fish meal diet (0% fish meal). The green liver was observed in all groups tested, but the incidence was much higher in the experimental diet groups. The feeding of substitute protein diets reduced plasma triglyceride and cholesterol levels. However, there was no significant difference in plasma hepatic enzyme activities and plasma bile salts concentration among the treatments. Fish fed the substitute protein diets showed low hepatic taurine levels with an appearance of a biliverdin in the liver. Moreover, the proportion of ditaurobilirubin to total biliary bile pigments was significantly lower in fish fed the substitute protein diets. These data indicate that feeding of substitute protein diets did not induce any cholestatic hepatobiliary obstructions and that the low hepatic taurine level was one of the probable factors responsible for the occurrence of green liver in red sea bream fed substitute protein diets.