新型角膜接触镜合成的研究

以甲基丙烯酸β-羟乙酯(HEM A)和N-乙烯基吡咯烷酮(NVP)为单体,过氧化苯甲酰(BPO)为引发剂,聚合成新型水凝胶角膜接触镜,并对此聚合物进行红外光谱分析,平衡含水量的分析,透氧量的分析和热重差热分析,发现这种材料符合日戴型角膜接触镜的要求。这一研究为药物载体的功能性角膜接触镜的研究奠定了基础。     

提高解剖镜放大倍数的一条新途径

[目的]为提高解剖镜的放大倍数和打破显微操作的极限开辟新途径。[方法]利用自制球形液滴的放大作用,对附地菜受精前、后的子房进行了观察和解剖,并用手工方法分离出了附地菜的胚珠和球形幼胚。[结果]在解剖镜下测得液滴内放大的附地菜子房的直径约为1．1mm;用吸水纸将液滴除去后,测得附地菜子房的实际直径约为0．7mm;球形液滴将附地菜子房放大了约1．57倍。球形液滴对光线有一定的聚集作用。在具有放大作用的球形液滴中,分离出受精后在胚珠中发育的球形幼胚（直径约0．1mm）,浅柒制片后,在显微镜下观察到幼胚的外形为球形。[结论]球形透明液滴可以提高解剖镜对液滴内物体或结构的放大倍数。     

Lens morphometry determined by B-mode ultrasonography of the normal and cataractous canine lens

Purpose To determine axial lens thickness, anterior chamber depth and axial globe length in canine eyes with normal lenses and in eyes with immature, mature, congenital, posterior polar and diabetic cataract. Methods B‐mode ultrasonography was performed in 50 normal dogs and, as a prephacoemulsification screening procedure, in 100 dogs with cataract. Axial B‐mode ultrasonograms were used to determine lens thickness, anterior chamber depth and globe diameter. Statistical comparisons between groups were made by analysis of variance and multivariate analysis, with a significance level of P < 0.05. Results Axial globe lengths were not statistically significantly different between groups apart from the smaller globes in younger dogs with congenital cataract. Axial lens thickness in diabetics (8.4 ± 0.9 mm) was statistically significantly different from the lens thickness in normal eyes (6.7 ± 1.0 mm), eyes with immature cataract (6.4 ± 0.8 mm) and eyes with mature cataract (7.4 ± 0.9 mm) although these groups, while varying in thickness, were not statistically significantly different from each other. Anterior chamber depth was statistically significantly reduced in eyes with diabetic cataract (2.9 ± 0.1 mm) from that in normal eyes (3.8 ± 0.1 mm), eyes with immature cataract (3.5 ± 0.1 mm) and eyes with mature cataract (3.2 ± 0.6 mm) although these groups, while varying in chamber depth, were not statistically significantly different from each other. Conclusions Lenses with diabetic cataracts were significantly increased in axial thickness compared to lenses in other eyes, although lenses with mature cataracts showed a trend towards increased axial thickness and immature cataracts demonstrated a trend towards reduced thickness. While previous studies on cataract pathobiology have suggested a reduction in lens thickness in immature cataract through lens protein loss and an increase in thickness in mature cataracts through intumescence, this study is the first to document these changes in the canine lens.     

Diagnostic ultrasonography of equine lens and posterior segment abnormalities

Purpose To define the indications for equine ocular ultrasonography and to provide representative ultrasonographic images of lens and posterior segment diseases. Methods Retrospective study. Equine medical records dating from January 1983 to March 2001 were reviewed. All cases that: (1) had a lens and/or posterior segment abnormality; and (2) received a complete ophthalmic examination and ocular ultrasonography were included. Results Forty‐three cases (n = 64 eyes) out of 112 total cases of equine lens and/or posterior segment abnormalities qualified. The following conditions were identified ultrasonographically in order of decreasing frequency: cataracts, vitreal opacities, retinal detachment, lens luxation, endophthalmitis, microphthalmos, choroiditis, lens rupture, lenticonus, buphthalmos and phthisis bulbi. Ultrasonography often enabled diagnoses to be made in the presence of anterior opacities in both surgical (pre and/or postoperatively) and nonsurgical cases. Additional ocular conditions were identified in adults with cataracts more frequently than in foals with cataracts. Conclusions Cataracts were the most common lens abnormality identified in horses that received ocular ultrasound examination. Cataracts were commonly found in association with other ocular abnormalities. Ultrasonography was a practical and effective method of evaluating the lens and posterior segment, particularly in cases with anterior opacities. Ultrasonography also provided critical information with regard to the potential for surgical removal of cataracts and was a valuable component of postsurgical follow‐up.     

The use of transscleral cyclophotocoagulation with a diode laser for the treatment of glaucoma occurring post intracapsular extraction of displaced lenses: a retrospective study of 15 dogs (1995-2000)

The objective of this retrospective study was to evaluate transscleral cyclophotocoagulation (TSCP) using a diode laser for treating aphakic glaucoma that developed after intracapsular lens extraction (ICLE) had been performed for displaced lenses. Records of 15 dogs (21 eyes) were reviewed. The intraocular pressure (IOP) and the presence of vision were recorded at the time of ICLE and TSCP and at 1, 3, 6-9, 12 and 24 months post TSCP. The glaucoma was considered controlled if the IOP was less than 25 mmHg. The results indicated that the effectiveness of TSCP using a diode laser for treating aphakic glaucoma was of a short-term duration (1-3 months), with three patients needing repeat TSPC. In addition, adjunctive antiglaucoma medications were often required to maintain an adequately controlled IOP. Over the 24-month period the number of dogs requiring intrascleral prosthesis, intraocular gentamicin or enucleation, or that were lost to follow-up, increased.     

Anterior lens capsule disruption and suspected malignant glaucoma in a dog

A Boston Terrier puppy presented with a full-thickness peripheral corneal defect, iris prolapse and anterior lens capsule tear in the left eye (OS). Phacofragmentation and primary repair of the corneal laceration was performed. At surgery, subluxation of the lens was also apparent. One day postoperative, there was severe corneal edema, diffuse hyphema, an intraocular pressure (IOP) of 65 mmHg and a small amount of vitreous that protruded from the corneal incision OS. Malignant glaucoma or pupillary block glaucoma were suspected. Intravenous mannitol was administered preoperatively and had no effect. An anterior vitrectomy was performed on the vitreous within the anterior chamber and pupil. One day postoperative the IOP was 16 mmHg in the right eye (OD) and 20 mmHg OS. Postoperative iridocyclitis was managed medically, and additional elevations in IOP were not recorded. Resolution of the elevated IOP following anterior vitrectomy was supportive of pupillary block or malignant glaucoma. Vision returned 3 weeks after the initial surgery. Two years after the initial injury, the eye is visual and comfortable with infrequent topical anti-inflammatory therapy.     

Ophthalmic Disorders Observed in Pet Chinchillas (Chinchilla lanigera)

This article presents a retrospective analysis of ocular disorders observed in outpatient pet chinchillas examined in a veterinary clinic between 2000 and 2010. The most common ophthalmic conditions diagnosed during this time period was alteration of the lens (15 eyes, 10 chinchillas), followed by abnormalities of the cornea (14 eyes, 13 chinchillas), and conjunctival disease (10 eyes, 9 chinchillas). The predominant lens disorder was cataracts, whereas the main alteration of the cornea was fluorescein-positive corneal substance loss. Subtle ocular changes appear to be common in the chinchilla. The data gathered within this study indicate that a thorough ophthalmic evaluation is warranted during routine physical examinations for this species.