注射用盐酸土霉素的细菌内毒素检查

参照<中国兽药典>二○○○年版一部附录细菌内毒素检查法,研究了注射用盐酸土霉素对细菌内毒素检测的干扰情况,确定0.312 5 mg/mL浓度的供试品溶液对检测无干扰作用,建立了注射用盐酸土霉素的细菌内毒素限量检查方法.     

规模化猪场大肠杆菌的耐药性监测及血清流行病学调查

为了调查猪致病性大肠杆菌的耐药性及流行血清型 ,从湖北、河南、江西、安徽、浙江等省的 33个猪场采集 32 1份仔猪黄、白痢病料进行细菌的分离和生化鉴定 ,结果分离到大肠杆菌 30 2株 ,其中致病性大肠杆菌 2 76株。对 112株致病性大肠杆菌进行了 15种抗生素敏感性试验 ,发现分离菌株对 15种药物均有不同程度的耐药性 :青霉素 G对其完全没有抑制作用 ;先锋霉素 抑制作用最强 (97.3% ) ,其次为呋喃妥因 (78.6 % )、痢特灵 (71.4 % )、环丙沙星(6 8.8% )、诺氟沙星 (6 5 .1% )。 112株菌中 ,有 4 7种耐药谱型 ,多数为 6耐以上的菌株 (80株 ) ,占供试菌株的 71.4 %。应用微量平板凝集试验 ,对分离的 2 76株致病菌进行了血清型鉴定 ,鉴定共有 72种血清型 ,其中优势血清型 10种 ,占能定型分离致病菌株的 5 3.7%。     

安宁河流域黑麦草、白三叶的生长测定

在最冷的12月至2月对四川凉山州安宁河流域的黑麦草、白三叶进行生长测定,结果证实,在当地建高产人工草地可作当家品种,冬季无停止生长期,最低产量可在5000kg以上,放牧利用的停牧周期在一月以内为好,预计年刈割周期次数为6～8次。     

Comparative studies of acetylcholinesterases purified from two field populations of the oriental migratory locust (Locusta migratoria manilensis): implications of insecticide resistance

Acetylcholinesterase (AChE) was purified by affinity chromatography from two populations of the oriental migratory locust, Locusta migratoria manilensis (Meyen), collected from Huanghua and Pingshan Counties, Hebei Province of China. The purification factors and yields were 1661-fold and 19.3%, respectively, for the Huanghua population, and 3897-fold and 39.6% for the Pingshan population. Both the purification factor and yield were significantly lower in the Huanghua population than in the Pingshan population. AChE activity was almost completely inhibited by 10⁻⁶ M eserine and BW284C51, but ?5.8% of AChE activity was inhibited by ethopropazine at the same concentration, suggesting that purified AChE from either population was a typical insect AChE. However, AChE purified from the Huanghua population was 62-, 2.0-, and 1.6-fold less sensitive to inhibition by the three organophosphate compounds, chlorpyrifos oxon, demeton-S-methyl, and paraoxon, respectively, than that from the Pingshan population. Significantly lower purification factor and low yield associated with reduced sensitivity of AChE to inhibition by the organophosphates indicated that AChE purified from the Huanghua population was biochemically and pharmacologically different from that of the Pingshan population. Reduced sensitivity of AChE appeared to contribute to organophosphate resistance in the locust from Huanghua County, where insecticides have commonly been used to manage outbreaks of the locust.     

水貂大肠埃希氏菌病的诊治试验

通过对患病水貂进行病理剖检,细菌纯培养,病毒检测,生化鉴定,确定是水貂大肠埃希氏菌病。再应用多种抗生素作药敏试验,筛选出了高度敏感的抗菌药。并对山东省威海地区水貂大肠埃希氏菌病的发生规律进行了探讨。     

湖南猪致病性大肠杆菌的分离及药物敏感性监测

从湖南47个规模化猪场发生典型仔猪黄、白痢猪只的63份腹泻病猪分离出63株大肠杆菌，其中12株作致病力试验均有致病性，对25种抗菌药物药敏试验，大肠杆菌对胺卡那霉素和头孢唑啉敏感，敏感菌株分别占98．41％和92．06％；多种抗生素产生耐药性，且为多重耐药。     

人参提取液体外抑制IHNV作用效果研究

探究人参提取液体外抑制CCB细胞感染IHNV的能力,构建体外CCB细胞模型,运用RTCA技术和MTT法测得不同浓度人参提取液对IHNV抑制作用曲线,并从阻断病毒复制和杀灭病毒两方面讨论人参提取液作用效果。结果表明,4%人参提取液对IHNV具有明显抑制作用,能够在16 h内保护细胞不受IHNV病毒的侵染,其中2 h时对病毒灭活抑制率最高达到51.5%,1~12 h内对病毒复制有持续抑制作用。研究可为IHVN防治药物的研发提供新的思路及参考。     

半滑舌鳎源美人鱼发光杆菌美人鱼亚种的分离鉴定

2016年5月河北省秦皇岛市昌黎县某海水养殖基地半滑舌鳎突发大批死亡,为了确定引起死亡的病原菌,无菌采集心血进行病原菌分离鉴定,16S rDNA基因序列分析与GenBank中公布的美人鱼发光杆菌参考株序列同源性为99.6%,被鉴定为美人鱼发光杆菌;进一步通过生化特性分析,被确认为美人鱼发光杆菌美人鱼亚种。动物回归试验结果表明,其对舌鳎鱼具有很强的致病性,其LD_(50)为3.1×10~4CFU/mL,且对哺乳动物具有致病性。药敏试验结果显示,分离菌对诺氟沙星、恩诺沙星、环丙沙星,乙酰甲喹高度敏感;对多西环素、阿米卡星中敏感;对庆大霉素、头孢曲松、替米考星等不敏感。