芍药苷对UVB诱导的小鼠色素沉着的改善与调控机制研究

为探讨芍药苷对皮肤色素沉着的改善作用及调控机制,采用UVB反复诱导方法建立C57小鼠皮肤色素沉着模型,评价芍药苷抑制皮肤色素沉着的作用及对酪氨酸酶(TYR)、小眼畸形相关转录因子(MITF)、转运蛋白RAB27A (RAB27A)、前阿黑皮素(POMC)、碱性成纤维细胞生长因子(FGF2)、干细胞因子(SCF)、白介素–1α(IL–1α)的表达水平的调控作用,同时利用分子对接法分析芍药苷与靶点之间的结合活性。结果表明：外用芍药苷降低了UVB诱导的C57小鼠的经皮失水率,皮肤组织切片中的表皮厚度和黑色素含量显著降低,皮肤组织中色素沉着相关的TYR、MITF、RAB27A蛋白的表达均极显著降低(P<0.01),黑色素细胞受体结合的旁分泌因子POMC、SCF、FGF2和炎症因子IL–1α的蛋白表达均极显著降低(P<0.01);分子对接结果显示,芍药苷对角质细胞分泌的POMC和SCF有极高的结合活性(活性总分值>7)。表明芍药苷可通过抑制旁分泌因子来抑制黑色素合成,发挥抑制皮肤色素沉着的作用。     

2014年上半年禽蛋市场形势分析与展望

2014年上半年,由于受流感疫情、节日效应、市场供求关系以及季节消费等多方面因素影响,全国鸡蛋零售价格波动频繁,1—6月鸡蛋均价为9.86元/kg,与2013年同比持平;蛋鸡配合饲料价格企稳,蛋鸡养殖行业总体处于盈利状态。预计,蛋价短期将呈高位震荡走势,中秋前后有望迎来新一轮上涨行情。     

实验室条件下o-烯丙基苯酚和p/o-丁酰基苯酚在土壤中的降解动力学研究

实验室条件下,在东北黑土、北京褐土、江西红土中添加o-烯丙基苯酚、p/o-丁酰基苯酚后对其降解进行了研究。结果发现,微生物对这2种农药在3种土壤中的降解起主要作用,它们在未灭菌土中的降解速度很快,符合一级动力学方程,T0.5在10.26h￣2.51d之间。两者均在有机质含量较高、pH偏碱性的东北土壤中降解最快;土壤含水量增加,降解速度减慢;外加碳源的存在,并没有加快它们的降解速度。     

慢生根瘤菌及其与花生共生机制研究进展

氮是植物生长发育所必需的大量元素之一,豆科植物通过与根瘤菌的共生固氮获得氮素.这种共生关系的建立包括结瘤和固氮两个过程,涉及复杂的互作调控机理,并受环境因素的显著影响.花生与慢生根瘤菌的共生对花生生产尤为重要,具有较多特异和未知的共生机制.本文综述了慢生根瘤菌及其与花生共生的相关内容,具体包括:(1)花生的慢生根瘤菌多...     

三北地区杨树速生林材积生长量与气候因子关系分析

应用灰色系统理论中的灰色关联度分析方法 ,对三北地区杨树速生林材积生长量与气候因子间的关系进行了定量分析 ,确定出影响材积生长量的主导气候因子为水分因子 ,光照因子次之 ,热量因子的影响最小     

Balance of Treg/Th17 in synovium of collagen-induced arthritis rats and impact of TNF-α blockage therapy

AIM: To investigate the balance of Treg/Th17 in synovium of collagen-induced arthritis (CIA) and the impact of tumor necrosis factor α(TNF-α) blockage therapy. METHODS: Rat CIA model was established by bovine II collagen injection. The pathological score was evaluated by HE staining and toluidine blue staining. The TNF-α level in plasma was measured by ELISA. The expression of Treg/Th17 in synovium was detected by double staining immunofluorescence. RESULTS: The plasma level of TNF-α in CIA group was significantly higher than that in control group and TNFR-Fc treatment group (P<0.01), whereas no significant difference was found between TNFR-Fc treatment group and control group (P>0.05). No significant difference between CIA group and control group in the ratio of CD4⁺Foxp3⁺Treg cells/CD4⁺ cells in synovium (23.12%±4.93% vs 24.66%±5.82%, P>0.05) was observed, whereas the ratio in TNFR-Fc treatment group was significantly increased(33.07%±5.14%). The ratio of CD4⁺RORγt⁺Th17 cells/CD4⁺ cells in CIA group was significantly higher than that in control group and TNFR-Fc treatment group (9.74%±2.23% vs 1.00%±0.59%, 5.63%±1.76%, P<0.01). CONCLUSION: Differentiation disturbance of Treg/Th17 exists in the synovium of CIA rats. TNFR-Fc may restore the balance of Treg/Th17 by inhibiting Th17 cell differentiation and inducing the production or accumulation of Treg.     

金黄色葡萄球菌聚集因子A（C1fa A）功能区基因的克隆及真核表达质粒构建

粘附素蛋白是金黄色葡萄球菌感染早期最重要的致病因子,研究根据金黄色葡萄球菌聚集因子A（c1fa A）基因,设计合成特异性引物,以国内奶牛乳腺炎临床分离株金黄色葡萄球菌基因组为PCR模板,进行c1fa A基因的克隆,并连接入pMD18-T载体进行测序和序列分析,然后经BamH I和Xba I双酶切后构建真核表达载体并进行鉴定。结果表明,以金黄色葡萄球菌标准株为对照,国内分离株多数在990bp处扩增到特异性条带,经测序鉴定,与Genebank发表的金黄色葡萄球菌c1fa A序列同源性为99％;构建的真核表达质粒通过PCR和双酶切鉴定证明构建成功,为研究核酸疫苗奠定基础。     

Effect of miR-181b on cell proliferation and expression of Bcl-2 and SP1 in HepG2 cells

AIM: To investigate the role of miR-181b in the expression of Bcl-2 and SP1 at mRNA and protein levels in the human hepatoma G2 cells (HepG2), and to explore the effect of miR-181b on the regulation of HepG2 cell proliferation. METHODS: The synthetic double-strand complementary DNA based on the sequence of miR-181b was inserted into the vector of miRNASelect^TM pEGP-miR. The microRNA high-expression plasmid was cloned, and the sequences were identified. The miR-181b plasmid was transfected into HepG2 cells with liposomes. The stable cell line was screened by puromycin. The mRNA and protein levels of Bcl-2 and SP1 were measured by RT-PCR and Western blotting, respectively. Methyl thiazolyl tetrazolium (MTT) method was used to analyze the proliferation of HepG2 cells. RESULTS: The Western blotting results showed that miR-181b inhibited the protein expression of Bcl-2 and SP1. The result of RT-PCR also indicated that the mRNA expression of Bcl-2 and SP1 was suppressed. Compared with the control, the growth rate of HepG2 with high expression of miR-181b was significantly decreased.CONCLUSION: miR-181b inhibits the proliferation of HepG2, which may be related to the down-regulation of Bcl-2 and SP1.     

科技期刊引文的分布与评价效用的分析与思考

目的 通过对中国30种科技期刊中外引文的数量及其各种分布情况的调查,揭示引文数量和质量与不同学术水平期刊之间的对应关系以及对中国科技期刊的评价效用。方法 采用统计分析方法,对不同学术水平期刊中外引文的数量和质量指标进行比较和分析。结果 学术期刊的引文数量和质量均高于普通期刊,而普通期刊由于中文引文比例大,对中国科技期刊学术评价的贡献度大于学术期刊,但学术评价效用不高。结论 引文作为论文的重要组成部分和学术质量的保证,应该纳入中国科技期刊综合评价指标体系中的独立指标,中国科学引文数据库来源期刊应进行适当调整,注重质量,可吸收一些相关的国际期刊。     

小麦生产TFP的区域比较分析

建立Tornqvist指数模型,计算了全国和河南、山东小麦生产的全要素生产率.结果表明,全国小麦总产增加主要来源于单产提高,而单产提高主要决定于全要素生产率增长,要素投入是小麦单产提高的物质基础.2001年以来,全要素生产率增长明显放缓,但河南省全要素生产率仍有较大优势.在压缩或稳定播种植面积的情况下,未来小麦总产增长关键是提高全要素生产率.