响应面法优化猕猴桃糖蛋白提取工艺研究

为确定乙醇浸提法提取猕猴桃糖蛋白的最优条件,采用响应面分析法,以液料比、乙醇体积分数、提取温度及提取时间为独立变量进行中心组合设计,以猕猴桃蛋白质提取率为响应值进行数学模型拟合,研究了各独立变量对猕猴桃糖蛋白提取率的影响。结果表明:当液料比为6.5∶1、乙醇体积分数24%、提取温度24℃、提取时间45min时,糖蛋白的提取率最高可达到63.05%,与试验设计结果相符,有助于设计猕猴桃蛋白质提取的最佳工艺。     

甘薯糖蛋白的免疫调节作用研究

初步研究了经ＤＥＡＥ５２和ＳｅｐｈａｄｅｘＧ１００柱层析纯化的北京２号薯糖蛋白的免疫的免疫调节作用。结果表明甘薯糖蛋白浓度达５０μｇ／ｍｌ时可促进ＰＨＡ人外周血淋巴细胞转化,１００μｇ／ｍｌ或者１５０μｇ／ｍｌ时间可显著提高ＰＨＡ刺激的人外周血淋巴细胞转化,刺激指数分别达到６．５和４．５（Ｐ〈０．０５）。腹腔注射甘薯糖蛋白８０ｍｇ／ｋｇ．ｄ可促进小鼠腹腔巨噬细胞的吞噬功能,吞噬指数和吞噬百分数均高     

表达狂犬病病毒糖蛋白的复制缺陷型重组腺病毒的构建

利用大肠杆菌内质粒间同源重组的方法,将狂犬病病毒G基因插入腺病毒基因组的E1基因区,构建了带有狂犬病病毒G基因的重组腺病毒质粒。重组质粒经Pme酶切,线性化后转染293细胞,成功获得了均一的复制缺陷型重组腺病毒。荧光显微镜下能观察到重组腺病毒GFP报告基因的表达。用PCR方法证实了重组腺病毒基因组中含有G基因,RT-PCR方法可检测到G基因的转录产物mRNA,Westernblotting方法能检测到重组腺病毒在29细胞中表达的G蛋白。此重组腺病毒在293细胞连续传代10次,PCR方法都能扩增出G基因目的条带。试验结果表明,狂犬病病毒G基因已成功重组到腺病毒基因组中,不但能稳定表达,而且能在重组腺病毒基因组中稳定存在。     

马动脉炎病毒大囊膜糖蛋白真核表达载体的构建与瞬时表达

将马动脉炎病毒大囊膜糖蛋白基因GL插入真核表达载体pVAX1中构建真核表达载体pVAX1-GL，酶切和测序结果表明构建是正确的，用脂质体转染试剂将其转染BHK-21细胞并通过问接免疫荧光试验检测其在体外的表达情况，结果在转染的细胞表面观察到绿色荧光，证明基因得到了表达，而对照则无绿色荧光，本研究为马动脉炎基因疫苗的研究奠定了基础。     

Preparation of an immunizing glycoprotein from inactivated rabies vaccine (author's transl)

Biochemical characteristics of rabies glycoproteins have been studied in the present work. Glycoproteins were prepared from rabies virions grown on different tissue culture origin. Isoelectrofocusing of rabies glycoprotein was achieved. The protective activity of rabies glycoprotein was assayed on laboratory animals. Results of neutralising antibody response correlated to resistance to challenge are discussed.     

The influence of dietary boron supplementation on egg quality and serum calcium, inorganic phosphorus, magnesium levels and alkaline phosphatase activity in laying hens

This study was performed to investigate the influence of dietary boron (B) supplementation on egg production, interior and exterior egg quality and serum parameters related to egg formation in laying hens. Two hundred and twenty four, 18 week old, Hyline Brown 98 strain hens were assigned to 7 groups with 4 replicates. After 10 days of adaptation the hens were fed for 8 weeks on diets supplemented with 0, 5, 10, 50, 100, 200 and 400 mg/kg (diet) B (H(3)BO(3)). Live weight, feed consumption and egg production were reduced in the 400 mg/kg B supplemented group. Increases were determined in serum Ca, inorganic P and Mg levels and serum ALP activity was not influenced by supplemental B. Boron changed the interior and exterior quality of eggs. In particular, high level of B supplementation reduced live weight, feed consumption and egg production and changed some blood metabolites.     

细菌糖蛋白对螯虾免疫因子的影响

实验检测了气味黄杆菌的胞外糖蛋白对克氏原螯虾（Ｐｒｏｃａｍｂａｒｕｓ ｃｌａｒｋｉｉ）的几咱非特异性免疫因子－凝集素、酚氧化酶、溶菌、抗菌和超氧化物歧化酶等活力的影响。注射１０ｍｇ糖蛋白后,实验组敖蛳主血清的免疫因子活力均高于对照组,凝集素活力在第１～４天最高;酚氧化酶在第１～５天维持较高活力,最大活力在第２天,溶菌、抗菌活力在第１～６天维持较高水平,分别在第３天、第２天达到最大。与对照组相比,实     

囊膜糖蛋白gE对α疱疹病毒毒力的影响

近年来，α疱疹病毒的囊膜糖蛋白gE在病毒细胞间传递、神经系统入侵、免疫逃避等方面的研究取得新的进展。gE能促进合胞体形成，影响病毒的顺行、逆行神经传导，也是第一个报道可以抑制浆细胞样树突状细胞产生Ⅰ型干扰素的病毒蛋白。本文对α疱疹病毒囊膜糖蛋白gE与毒力之间的关系进行阐述，以期为α疱疹病毒gE的功能研究提供参考。