全文获取类型
收费全文 | 16063篇 |
免费 | 1195篇 |
国内免费 | 1746篇 |
专业分类
林业 | 1604篇 |
农学 | 2704篇 |
基础科学 | 463篇 |
1792篇 | |
综合类 | 6379篇 |
农作物 | 1056篇 |
水产渔业 | 1307篇 |
畜牧兽医 | 2194篇 |
园艺 | 473篇 |
植物保护 | 1032篇 |
出版年
2024年 | 75篇 |
2023年 | 254篇 |
2022年 | 509篇 |
2021年 | 521篇 |
2020年 | 585篇 |
2019年 | 669篇 |
2018年 | 477篇 |
2017年 | 718篇 |
2016年 | 815篇 |
2015年 | 780篇 |
2014年 | 783篇 |
2013年 | 864篇 |
2012年 | 1170篇 |
2011年 | 1178篇 |
2010年 | 998篇 |
2009年 | 933篇 |
2008年 | 862篇 |
2007年 | 918篇 |
2006年 | 804篇 |
2005年 | 663篇 |
2004年 | 537篇 |
2003年 | 474篇 |
2002年 | 391篇 |
2001年 | 367篇 |
2000年 | 303篇 |
1999年 | 282篇 |
1998年 | 234篇 |
1997年 | 216篇 |
1996年 | 229篇 |
1995年 | 207篇 |
1994年 | 203篇 |
1993年 | 176篇 |
1992年 | 153篇 |
1991年 | 129篇 |
1990年 | 126篇 |
1989年 | 85篇 |
1988年 | 61篇 |
1987年 | 59篇 |
1986年 | 39篇 |
1985年 | 18篇 |
1984年 | 22篇 |
1983年 | 8篇 |
1982年 | 10篇 |
1981年 | 14篇 |
1980年 | 7篇 |
1979年 | 11篇 |
1978年 | 17篇 |
1977年 | 14篇 |
1976年 | 9篇 |
1955年 | 18篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
31.
M. Bonierbale R. Plaisted S. Tanksley 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(Z2):211-214
Introgression of trichome-mediated insect resistance from the wild speciesSolanum berthaultii has become a major focus of the potato improvement program at Cornell University during the past twelve years. Several quantitative characters are involved in this resistance which is effective against a wide range of pest types. Correlative biochemical assays have been developed to assay specific components of the resistance, and the effects of the resistance on the target pests have been studied. Quantitative laboratory assays and specific measurements of insect behavior and biology have increased the precision of selection and enable the investigation of the genetic control of the resistance.We are currently using restriction fragment length polymorphisms (RFLPs) for genetic mapping of factors controlling the trichome traits fromS. berthaultii. Backcrosses to both the wild and the cultivated species parents have been evaluated for phenotypes contributing to the resistance mechanism, including trichome density, sucrose ester and polyphenol oxidase production by the trichomes, and the enzymatic browning reaction responsible for insect entrapment. Genetic maps are being developed for these progenies, using RFLP markers previously mapped in potato. Field and greenhouse trials under insect infestations are also being conducted with the mapping progeny. Our goal is to locate genes responsible for quantitative insect resistance by correlating RFLP variation at mapped loci with the trichome phenotypes and insect resistance. Genetic markers for these traits will be useful in transfer of the effective wild chromosomal segments into and among tetraploid potatoes, and for a better understanding of the resistance mechanism. 相似文献
32.
本文论述了中国山羊的起源、品种遗传资源及其遗传多样性的研究进展。最新调查 显示中国的山羊品种达50个,其中43个为地方品种,4个培育品种,3个引入品种,许多品种 具有独特的生产性能或适应能力。大量研究表明,我国山羊遗传多样性较为丰富。 相似文献
33.
节能日光温室光照强度的分布及其变化 总被引:8,自引:0,他引:8
在充分考虑了温室结构和植株生长影响的基础上,对日光温室光照强度的变化规律进行了研究,得到的主要结论如下:冠层上部辐射强度的南北差异比东西差异大;冠层下部光照条件东西差异明显,南北差异不大。总体上讲,辐射强度上下差异比南北差异明显。 相似文献
34.
甘肃省河东地区主秋期降水量时空分布特征 总被引:3,自引:1,他引:3
利用甘肃省河东地区55个站30a的主秋期降水量资料进行经验正交展开(EOF)和旋转经验正交展开(REOF),分析其空间分布特征和异常类型;以平凉站为例分析了主秋期降水的时间变化规律和周期性;将河东地区主秋期降水量分为8个气候片。分析表明,河东地区主秋期雨量变化规律较为一致,空间分布不均,具有南多北少和东西多中间少的特点;多雨中心和少雨中心的多年平均值相差近200mm;主秋期降水年际变化较大,容易出现不同程度的旱或涝,并有9a左右的周期;20世纪40、60年代为相对多雨期,50、90年代为相对少雨期,70、80年代与平均值持平。 相似文献
35.
Genetic variability within Phaeoisariopsis griseola from Central America and its implications for resistance breeding of common bean 总被引:1,自引:0,他引:1
The genetic and virulence variability of 112 isolates of Phaeoisariopsis griseola , collected from various locations in Central America, were studied using seven random amplified polymorphic DNA (RAPD) primers and 12 common-bean differential genotypes. Broad molecular diversity ( H = 0·92) among isolates was found using RAPD markers. Fifty pathotypes were identified on 12 differential bean genotypes, 29 of which were represented by only one isolate. Only 18 pathotypes were found in two or more countries. Pathotype 63-63 was the most virulent and caused leaf spots on all 12 common-bean differential genotypes. Comparison of virulence phenotypes and RAPD profiles to known Andean P. griseola isolates confirmed that all isolates belonged to the Mesoamerican group. Pairwise comparison between individual RAPD loci showed that the majority were in gametic phase linkage disequilibrium, revealing that P. griseola maintains a genetic structure that is consistent with asexual reproduction. The molecular and virulence diversities of P. griseola isolates from Central America imply that using single resistance genes to manage angular leaf spot is inadequate and stacking resistance genes may be necessary to manage the disease effectively. 相似文献
36.
Variation of Parthenium hysterophorus in response to soil quality: implications for invasiveness 总被引:1,自引:0,他引:1
Summary The performance of Parthenium hysterophorus , native to the tropical Americas and invasive to several countries, was evaluated for response to soil quality. Phenological (six stages) and quantitative growth variables [relative growth rates in height (Rh ) and diameter (Rd )] were measured every fortnight. Based on harvest data, the variables, root:shoot (R:S) ratio, specific leaf area (SLA), relative growth rate in biomass (Rw ), net assimilation rate (NAR) and dry matter allocation to plant components, were determined. High clay content in soils prolonged the rosette stage, enhanced Rh and Rd and hampered root growth, but promoted biomass allocation to shoots. The extreme degree of plant mortality was observed, with only 33.3% individuals surviving to reproduce in the soils with highest clay contents. This appears to be one of the most important findings that certainly has a major bearing on the range of adaptation of the weed. Seed mass declined whereas seed production increased in relatively coarser soils with the exception of nearly pure sand. In soils rich in clay, plants produced a smaller number of larger seeds. These data suggest that variation in fundamental functional traits would enable P. hysterophorus to adjust to a variety of habitat conditions. 相似文献
37.
M. C. C. de Arruda † R. N. G. Miller M. A. S. V. Ferreira M. S. S. Felipe 《Plant pathology》2003,52(2):236-244
Fifty isolates of Crinipellis perniciosa originating from Theobroma cacao , Heteropterys acutifolia and Solanum lycocarpum , from six states within Brazil, were characterized through ERIC-PCR, representing the first application of this method for molecular characterization within C. perniciosa . Phenetic analysis of banding patterns revealed a separation of isolates on the basis of host of origin, with T. cacao -derived isolates showing only a 0·2 similarity level to a cluster comprising the isolates from H. acutifolia and S. lycocarpum . Considerable intraspecific variability was observed within C. perniciosa isolates from T. cacao , with distinct groups observed correlating with geographical origin. Given that a number of isolates from T. cacao from the Amazon region grouped with isolates from Bahia state, this work discusses the possibility that current C. perniciosa populations pathogenic on T. cacao in Bahia originated from the Amazon region, rather than from alternative host plants. 相似文献
38.
SSR-based genetic linkage analysis of resistance to crown rust (Puccinia coronata f. sp. lolii) in perennial ryegrass (Lolium perenne) 总被引:2,自引:0,他引:2
Crown rust (caused by Puccinia coronata f. sp. lolii) is a serious foliar disease of the pasture and turfgrass perennial ryegrass (Lolium perenne). Previous genetic studies have detected both qualitative and quantitative resistance mechanisms, and interpretation of the genetic system is complicated by variation within the sexually reproducing pathogen. Resistant and susceptible parental genotypes of ryegrass were identified using a composite urediniospore population collected from three geographically distinct locations. A two-way pseudo-testcross mapping population was obtained as the F1 progeny of the pair-cross between ryegrass parental genotypes Vedette6 and Victorian9. Both parents showed intermediate resistance against a pathogen population collected in a single geographical zone (Hamilton, Victoria), but in the F1 population, significant variation for a range of resistance-associated characters was detected. Statistical analysis of phenotypic data suggested a major gene effect, hence bulked segregant analysis with map-assigned simple sequence repeat (SSR) markers was used to scan the genome. A marker showing strong association with resistance was assigned to linkage group (LG) 2 of perennial ryegrass. Analysis of 11 LG2 SSR markers defined an interval between loci xlpssrh03f03 and xlpssrk02e02 as containing the gene or genes (LpPc1) conferring crown rust resistance. Resistance gene determinants were inherited from both parents, with up to 80% of the total phenotypic variation explained by markers segregating from Vedette6 and up to 26% of the variation explained by markers segregating from Victorian9. The two contributions together resulted in an additive increase in effect, with fully resistant individuals requiring determinants from both parents. A conserved syntenic relationship was observed with linkage group B of Avena strigosa, which is the location of a cluster of resistance genes to the oat form of crown rust. The implications of this study for marker-assisted selection of disease resistance in perennial ryegrass are discussed. 相似文献
39.
AFLP是一种新的分子遗传标记技术,它结合了RFLP和PCR技术的优点,具有RFLP的稳定性和PCR的高效性,被称为“最有威力的分子遗传标记”或“下一代分子标记”。相对于国外而言,我国在这方面的研究还比较少。本文综述了扩增片段长度多态性(AFLP)这种遗传标记的原理、技术特点、技术发展及其在动物遗传多样性、种质鉴定、遗传图谱构建、基因定位和标记辅助育种等研究中的应用。 相似文献
40.