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21.
Update on the diagnosis of Haemophilus parasuis infection in pigs and novel genotyping methods 总被引:1,自引:0,他引:1
Haemophilus parasuis causes Gl?sser's disease as well as a number of other diseases in pigs. The diagnosis of H. parasuis-associated disease is usually established by clinical signs, pathological findings and bacterial isolation but diagnosis is complicated by the existence of non-virulent strains and the early colonisation of the upper respiratory tract of healthy piglets. Moreover, several strains can be found on a farm and even within a single animal so it is important to determine the specific strain that is causing the clinical outbreak. Recently, genotyping methods have been developed with the goal of correlating genotype with the degree of virulence of H. parasuis strains. The association between genotype and virulence in H. parasuis is challenging due to the lack of knowledge of the complete genomic sequence and virulence factors of this bacterium. 相似文献
22.
Murrah buffaloes, best breed for milk production are native of Haryana state. They contributes significantly to the farmer’s income, livelihood and food (milk and meat) security, in the semi-tropical regions of North India. Johne’s disease though endemic in the domestic livestock of the country, but reports are not available in the buffaloes suffering from morbidity due to progressive weakness and diarrhoea. We estimated the status of JD in diarrhoeic buffaloes and cattle reporting at Veterinary Clinical Complex of Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India, using conventional, serological and PCR assays.141 buffaloes suffering from chronic diarrhoea were screened to estimate sero-prevalence of MAP and 50.0 % young and 53.52 % adult animals were positive. Of 14 cattle screened, none of the young and 66.6 % adult cows were positive. In buffaloes, 66.1 and 6.77 %, fecal samples were positive in microscopy and IS900 PCR, respectively. Sero-prevalence of JD was very high in diarrhoeic buffaloes and cattle from Haryana state of India.Buffaloes positive for Mycobacterium avium subspecies paratuberculosis (MAP) infection had reduced total leukocyte count and lymphocytes. 相似文献
23.
为解决人工饲料育的发育不齐问题,就饲料的不同调制方法对桑蚕幼虫发育的影响,以及同一蚕品种个体间的差异进行了研究。结果发现:①在饲料制作上必须进行蒸煮灭菌,以微波炉加热灭菌的与蒸煮的效果相近,不经加热杀菌简易化制作的效果都不理想;②同一蚕品种个体间对人工饲料的摄食性存在差异。对桑叶粉及蔗糖等摄食促进物质的感受性,高食性蚕比低食性蚕敏感;而对豆粕粉中摄食阻碍物质的感受性,低食性蚕比高食性蚕敏感。研究认为,要提高人工饲料育蚕的群体摄食性和发育整齐度,应尽量消除饲料中的摄食阻碍物质,或选育出对摄食阻碍物质具适应性的蚕品种 相似文献
24.
为评价牦牛奶渣乳酸茵对藕草青贮饲料发酵品质和化学成分的影响,试验设置对照组(CK)和3个不同乳酸茵含量处理组(Nzl:0.1ml·kg-1FM;NZ2:0.3ml·kg-1FM;NZ3:0.5ml·kg—FM),青贮60d后检测发酵品质和化学成分。试验表明:不同添加剂比例对藕草青贮饲料影响不同;NZ1和NZ3处理能够显著降低祷草青贮饲料的pH(P〈0.05),具有较高的乳酸和乙酸含量(P〈0.05),NZ3处理的中性、酸性洗涤纤维含量显著降低(P〈0.05),说明牦牛奶渣乳酸茵可以改蒿草青贮饲料的发酵品质;随着添加浓度的增加赫草青贮饲料的可溶性碳水化合物和粗蛋白具有显著增加趋势,中、酸性洗涤纤维具有降低趋势,说明牦牛奶渣乳酸菌能够改籍草青贮饲料的营养价值;牦牛奶渣乳酸茵的最适添加量为0.5ml·kg-1FM。 相似文献
25.
26.
Rama G Chhina DK Chhina RS Sharma S 《Comparative immunology, microbiology and infectious diseases》2005,28(5-6):339-349
The human urinary tract is able to combat with the microbial invasion under normal circumstances. To cause urinary tract infection the organism has to evade the host defense mechanisms by possessing distinct properties which contribute to the virulence of the organism hence called virulence determinants Ninety percent of uncomplicated urinary tract infections are caused by Escherichia coli, hence the knowledge of the virulence determinants of this organism can be extrapolated to other uropathogenic organism as well. Virulence determinants of uropathogenic E. coli include adhesins, siderophore production, polysaccharide coating, hemolysin production, outer membrane proteins etc. The intestinal E. coli, which are the reservoir of E. coli for causing UTI, lack these virulence determinants. On the other hand these virulence determinants enable the organism to colonize and invade the urinary tract. In addition these are important in acquiring the nutrients in other wise nutrient deficient environment. Further, they also help the organisms in triggering an inflammatory response and hence bringing about pathological changes which leads to symptomatic UTI. Severity of symptomatic infections and tissue damage during the infective process depends upon the magnitude of the inflammatory response triggered by the uropathogen which in turn is dependent upon the amount of extrcellular release of reactive oxygen species by the phagocytic cells; hence role of antioxidants as an adjunct to antibiotics in the treatment of infective process needs to be evaluated further. 相似文献
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28.
单股正链RNA病毒数量众多,对人、动物和植物造成很大的危害。病毒基因组3'末端都具有一段非编码区,基因组3'非编码区在病毒的复制过程中发挥着重要的作用。本文简单介绍了单股正链RNA病毒基因组3'非编码区结构的预测、测定和功能的研究方法,并重点概括了不同单股正链RNA病毒3'非编码区一级序列、茎-环结构、假结体、TLS等结构在病毒基因组的复制、转录和翻译中的调控作用以及目前存在的问题。 相似文献
29.
不同鹿胎及胎盘制剂对老年雄性大鼠免疫机能影响的研究 总被引:3,自引:1,他引:3
研究了鹿胎及胎盘制剂对老年雄性大鼠免疫器官指数、血清球蛋白含量和巨噬细胞吞噬率及吞噬指数的影响。结果表明,鹿胎及胎盘制剂对老年雄性大鼠胸腺和脾脏指数影响不显著(P>0.05);但提高了老年雄性大鼠血清球蛋白含量,其中鹿胎制剂Ⅰ号组最高,比对照组高出62.64%,与其他各组相比有显著差异(P<0.05);鹿胎制剂Ⅱ号组和人胎盘粉处理组大鼠巨噬细胞吞噬率和吞噬指数与对照组相比有极显著提高(P<0.01),说明鹿胎及胎盘制剂对老年雄性大鼠具有提高免疫机能的作用。 相似文献
30.