长期定位施肥土壤酶活性及其肥力变化研究

对小麦-玉米轮作区长期定位施肥土壤酶活性及其肥力变化的研究结果表明,与未施肥(对照)相比,长期施肥处理土壤脲酶、碱性磷酸酶、转化酶和过氧化氢酶活性均明显提高,其中有机肥(秸秆、厩肥)配施化肥效果明显优于单施化肥;施肥后休闲处理未提高土壤酶活性,表明施肥(化肥、有机肥)后作物根系及其分泌物具有刺激土壤酶活性的作用,且土壤酶活性高低与作物产量(即土壤肥力)相关性显著.     

CO2浓度与氮磷供应水平对黄瓜根系生长及各组织矿质养分含量的影响

在开顶式生长箱内，以黄瓜为试验材料，采用营养液培养方法，研究了不同氮水平、磷水平条件下大气CO2浓度对黄瓜植株内矿质养分含量以及根系形态的影响。结果表明：黄瓜植株各部位氮素含量随供氮水平提高而增加，磷水平提高，也能促进各部位氮含量的提高。植株各部位磷含量随供磷水平的提高而升高，在相同磷水平下，缺氮会使各部位磷含量升高。大气CO2浓度升高会使黄瓜植株各部位氮及特定部位的磷含量降低。黄瓜根部的Ca含量随CO2浓度的升高而显著降低，氮和磷水平的升高极显著地增加了其含量，且CO2浓度与供磷水平、供氮与供磷水平以及这三者之间存在明显的交互作用。供氮、供磷水平的升高极显著的提高了黄瓜叶片Ca的含量以及茎部Mg的含量，且两者存在明显的交互作用。黄瓜总根长和总根表面积随CO2浓度的增加有增大的趋势；在缺磷条件下，总根长和总根表面积随氮水平的提高而增大；而同一氮水平和CO2浓度下，磷水平的降低会增加总根长和总根表面积。总体看来，大气CO2浓度的升高能促进黄瓜根系的生长，但会使得黄瓜植株某些部位氮、磷、钙、镁等矿质元素含量降低，而供氮、供磷水平的提高可以通过增强黄瓜的生长与活力促进黄瓜根系对矿质养分的吸收，从而缓解由于CO2浓度升高带来的矿质元素含量降低的风险。这启示我们在对设施蔬菜CO2施肥的同时，也要注重适量提高合理配比下矿质元素的供应。     

冷型小麦磷素吸收积累特性的研究

2002至2004年,通过田间小区试验,研究了4种施肥条件下(不施肥、单施磷肥、单施氮肥和氮磷配施)冷型小麦的磷素吸收积累特性。结果表明,与暖型小麦相比,冷型小麦开花前功能叶片和穗部具有较高的磷素积累量;开花后的吸收积累量也较大,比暖型小麦高24.4%;成熟期冷型小麦子粒中磷素积累量在不施肥、单施磷肥、单施氮肥和氮磷配施条件下,分别比暖型小麦高6.3%、11.1%、18.5%和50.6%;植株磷素总积累量趋势与子粒一致。结果说明冷型小麦属于高效吸磷基因型,暖型小麦属于低效吸磷基因型;而且冷型小麦磷素积累量对氮、磷肥均有较高的响应度,属于高效高响应吸磷基因型。     

基于局部二值模式和灰度共生矩阵的籽棉杂质分类识别

籽棉杂质的分类识别是实现棉花生产线自适应加工的基础与重要依据。该文提出了一种基于局部二值模式和灰度共生矩阵的籽棉杂质分类识别算法,该算法将含杂籽棉图像首先转换为局部二值模式图像,获取图像的微观结构,再用局部二值模式图像生成灰度共生矩阵并计算特征参数,获取图像宏观结构。使用支持向量机作为分类器,用不同尺度的图像结构进行训练,从而达到籽棉杂质的分类识别。试验结果表明,该文设计算法对各种杂质的平均正确识别率达到了94%,超过单独使用局部二值模式和单独使用灰度共生矩阵的正确识别率,为实现棉花自适应加工提供了技术基础。     

Biological soil amelioration as the basis of sustainable agriculture and forestry

Before the discovery of plant nutrients, soil amelioration and fertilization was by force of circumstance biological, i.e. done with substances taken from nature. After the discovery of plant nutrients, the chemical concept of fertility displaced the biological one. The defects of chemical cultivation — inefficiency, deterioration in product quality, diminishing productivity of soils and negative effects on the environment — have created an urgent need for the study of fertility as a result of the activity of the biocycles of the ecosystem. With the aid of the advances of modern science, we can understand the defects and deficiencies of the chemical concept of fertility and return the biological concept of fertility to its proper place. This in turn is a good starting point for productive research and developmental work towards an ecologically sustainable agriculture and forestry.     

施肥与覆膜对棕壤Olsen-P剖面分布及动态变化的影响

利用沈阳农业大学长期定位试验站裸地和覆膜的不同施肥处理（不施肥、单施氮肥、有机肥、有机无机肥配施）及其附近不同土地利用方式，探讨了不同施肥处理和覆膜对整个生长季的棕壤Olsen-P剖面（0—100 cm）分布及动态变化的影响。结果表明，耕地棕壤各施肥处理土壤剖面Olsen-P含量均表现为0—20 cm和60—100 cm大于20—60 cm；而自然草地、自然林地棕壤Olsen-P含量则随深度加深（0—100 cm）而逐渐增多。施用有机肥或有机无机肥配施处理的Olsen-P含量在60 cm土层以上均远大于不施肥或单施氮肥处理；覆膜后不施肥、有机肥、有机无机肥配施处理土壤0—40 cm土层的Olsen-P含量略有降低（0—20 cm土层有机肥处理除外），但差异未达到显著水平。说明施有机肥或有机无机肥配施，是补充土壤Olsen-P的有效措施；但施肥时应深施以补充表层以下土壤Olsen-P含量。覆膜未对本研究的棕壤Olsen-P含量产生显著的负面影响。     

利用改善液培养牛卵母细胞和早期胚胎的研究

在基础液 M199配制的培养液中,加入牛卵泡内的颗料细胞,置39℃、5%的 CO_2培养箱中培养72 h 后回收过滤液(简称改善液)。用这种不同浓度和保存时间的改善液,在体外成熟培养牛卵母细胞和胚胎时分别代替培养液和培养液加颗粒细胞制作的单层细胞培养体系。结果表明:(1)在基础液 M199中分别加入0%、25%、50%、75%、100%改善液和对照纽,其分裂率(78.7%～83.0%)组间均无差异(P>0.05);受精卵在体外培养至囊胚率,其中0%改善液组为30.4%,与对照组46.5%有显著差异(P<0.01);囊胚孵化率0%和25%改善液组分别为57.1%和57.9%,与对照组76.6%有显著差异(P<0.01)。(2)改善液保存时间为0 d,-4℃ 5～7 d,-20℃180 d 和对照组,分裂率(80.5%～86.3%)组间均无差异(P>0.05);受精卵在体外培养至囊胚率和囊胚孵化率,其中-20℃保存180 d 组分别为31.1%和54.5%,与对照组46.1%和72.0%有差异(P<0.05)。     

Increased N availability in grassland soils modifies their microbial communities and decreases the abundance of arbuscular mycorrhizal fungi

Two complementary studies were performed to examine (1) the effect of 18 years of nitrogen (N) fertilization, and (2) the effects of N fertilization during one growing season on soil microbial community composition and soil resource availability in a grassland ecosystem. N was added at three different rates: 0, 5.44, and 27.2 g N m⁻² y⁻¹. In both studies, Schizachyrium scoparium was the dominant plant species before N treatments were applied. Soil microbial communities from each experiment were characterized using fatty acid methyl ester (FAME) analysis. Discriminant analysis of the FAMEs separated the three N fertilizer treatments in both experiments, indicating shifts in the composition of the microbial communities. In general, plots that received N fertilizer at low or high application rates for 18 years showed increased proportions of bacterial FAMEs and decreased fungal FAMEs. In particular, control plots contained a significantly higher proportion of fungal FAMEs C18:1(cis9) and C18:2(cis9,12) and of the arbuscular mycorrhizal fungal (AMF) FAME, C16:1(cis11), than both of the N addition treatment plots. A significant negative effect of N fertilization on the AMF FAME, C16:1(cis11), was measured in the short-term experiment. Our results indicate that high rates of anthropogenic N deposition can lead to significant changes in the composition of soil microbial communities over short periods and can even disrupt the relationship between AMF and plants.     

Urease activity in two cultivated and non-cultivated arid soils

Summary Since urease activity in soil is believed to be relatively constant, the present study was designed to examine the effects of incubation, soil depth and the effect of cultivation on the persistence of urease activity in arid soils. Two soils were used, a Harkey (coarse, silty, mixed, calcareous, thermic, Typic Torrifluvent) and a Saneli (Clayey over sandy skeletal, montmorillonitic, calcareous, Vertic Torrifluvent), each consisting of a cultivated field and a non-cultivated roadbed site. Urease activity was much lower and more varable in the roadbed soils (40 years without cultivation) than in the cultivated field soils. Pre-incubation for 24 h with urea (with toluene) and without urea (without toluene) greatly reduced the total urease activity in all cases in relation to cell free urease activity (with toluene). Urease activity in the two field soils decreased slightly with profile depth but the decrease was greatest below the plow depth (33 cm). Protease activity or some inactivation processes must have lowered the urease content since there was substantially reduced urease activity after most pre-incubations. The extent of the urease activity decrease was so great that the addition of urea would have been required to increase the production of urease enzyme.     

长期施用有机和无机肥对潮土氮素平衡与去向的影响

通过13年26季不同施肥方式对潮土土壤氮素平衡及去向进行定位监测的结果表明,不施氮肥土壤平均每年接受外源氮为N.23～24.kg/hm2,小麦和玉米带走N.82～88.kg/hm2,亏缺N.58.6～63.7.kg/hm2;各施氮肥处理平均每年实际盈余N.238.6～418.kg/hm2,其盈余多少顺序为1.5MNPKNNKMNPK(2)MNPKNPKNPSNPK。N、NK处理土壤接受外源氮N为4881和4876.kg/hm2,其中14.1%～20.7%被作物吸收利用,17.2%和5.2%残留在土壤,64%和72%损失;NP、NPK处理氮素去向基本一致,48%被作物利用,9.7%的残留在土壤中,55%损失;有机肥处理(MNPK)的氮素利用率、残留率和损失率分别为44.3%、23%和42%;有机肥用量增加,氮素利用率降低,损失率增加;秸秆还田(SNPK)氮素利用率最高,为51%;NK处理氮素损失最大。