Molecular investigation on the presence of canine parvovirus in Egypt

Canine parvovirus type 2 (CPV-2) causes a highly contagious gastroenteritis disease of dogs and wild canids. To investigate the CPV-2 prevalence in Dakahlia Governorate, Egypt, a total of 50 fecal swabs were collected from suspected diseased dogs during 2016–2017. Out of 50 collected samples, 35 samples (70 %) presented positive results for CPV-2 using immuno-chromatography (IC) as a rapid test. CPV-2DNA was detected in 42 samples (84 %) by using polymerase chain reaction (PCR). The frequencies of CPV-2 were significantly higher in German shepherd breed (46 %; 23/50) and in age groups less than 6 months (76%; 38/50). We evaluated the breed, age, sex, rapid test results and clinical signs as predictors for classification of animal status into infected and not infected. The best predictors for classification process were rapid test result and clinical signs. Both CPV-2b and CPV-2c subtypes were detected by CPV2-VP2 gene sequences analysis. Deduced amino acid sequences alignment showed substitutions at 3 sites (Arg453Pro, Ala574Glu and Gln457Leu). Further investigations are needed to reveal the genetic and antigenic relation between field and vaccinal strains of CPV-2 in Egypt.     

猎豹与虎猫杯状病毒的分离及其超变区基因比较研究

用F81细胞从上海某动物园患口腔溃疡的猎豹和虎的唾液病料中分离获得两株杯状病毒，经形态学、理化学、生物学鉴定和病毒核酸超变区基因RT-PCR扩增与序列测定证明两株病毒均为猫杯状病毒(FCV)，分别命名为FCV/cheetah/Shanghai/02/2002与FCV／tiger/Shanghai／03/2002。人工感染猫可引起体温升高，口腔溃疡，食欲下降等症状。两株病毒的超变区序列520bp长片段问的同源性为99.2％，与国内桂林虎分离株(TFCV9710)的同源性为74．0％，与国外分离株的总体同源性为58．1％，说明不同宿主或同种不同个体间杯状病毒分离株超变区核酸差异十分显著，符合猫杯状病毒的分子生物学特征。     

西宁地区猪细小病毒病的血清学调查

应用血球凝集抑制试验对青海省西宁地区的某规模化猪场的96份血清样品进行细小病毒病的血清学调查.结果：检出阳性头数8份，阳性率为83％(8／96)     

ULTRASONOGRAPHY OF ALIMENTARY LYMPHOSARCOMA IN THE CAT

Clinical, radiological and ultrasonographic findings from 22 cats with gastrointestinal lymphosarcoma were reviewed. The most common clinical findings were anorexia, weight loss, vomiting, lethargy, depression, fever, anemia and a palpable abdominal mass. Abdominal radiographs of 12 cats revealed a mass associated with the gastrointestinal tract and/or mesentery, and decreased serosal detail, suggestive of peritoneal effusion. The most common ultrasonographic finding was transmural gastric or intestinal thickening associated with loss of normal wall layering, reduced wall echogenicity and localized hypomotility. As is noted in people, other patterns such as transmural-segmental, transmural-nodular, transmural-bulky and mucosal infiltration were represented. In 3 cats, mesenteric lymph-adenopathy was responsible for most of the mass-effect. Fine-needle aspiration biopsy, automated microcore biopsy, endoscopic biopsy or necropsy confirmed the diagnosis of lymphosarcoma in all cats. Chemotherapy was instituted in 19 cats. Only 6 cats had a complete response to treatment. The results of this study indicate that ultrasonography is a valuable tool for the diagnosis of feline alimentary lymphosarcoma and that biopsies can be obtained under ultrasonographic guidance.     

Feline Ubiquitin Fusion Protein Genes

Using cDNA from a CRFK cell line as a template, PCR amplification was performed with the Ub1S and poly(dT) primers to isolate feline ubiquitin genes. Sequencing of the 495 bp PCR fragment revealed that the putative amino acids induced by this fragment gave a fusion protein consisting of a ubiquitin polypeptide (76 amino acids) and an extension protein of ribosomal proteins L40 (52 amino acids). The putative amino acid sequence of ubiquitin was identical to those of humans, rats and pigs.The recombinant glutathione S-transferase (GST)–feline ubiquitin fusion proteins were produced in Escherichia coli and purified. The fusion proteins had a molecular weight of about 42 kDa and were detected by immunoblot assay with rabbit anti-ubiquitin antiserum.The mRNAs from heat-shocked and non-heat-shocked cells were subjected to RT-PCR (Ub1S and poly(dT) primers) analysis. The molecular weights of the ubiquitinated proteins in heat-shocked CFRK cells were between 18 kDa and 24 kDa by immunoblot assay.These results suggested that there were more ubiquinated proteins in the heat-shocked CRFK cells than in the pre-heat-shocked cells.     

牛磺酸在猫营养中的应用

主要综述近年猫的牛磺酸营养,从研究方法来看衡量牛磺酸利用的方法与指标已较完善,对牛磺酸的代谢有了深入的了解,并进一步关注猫肠道菌群影响牛磺酸代谢的机理的研究;初步揭示大豆蛋白和脂肪等营养物质降低牛磺酸利用率的原因;在猫粮加工方面指出不良热处理导致的猫粮中牛磺酸利用率降低的机制,并分析了一些不适合配制猫粮的原料如米糠、大豆蛋白对牛磺酸代谢的影响;以及在猫粮中直接添加晶体牛磺酸的重要性。从文中可以看到,作为必需氨基酸的牛磺酸对于猫的生长繁殖来说十分重要。     

鹅细小病毒HG5/82株的分离鉴定及生物学特性的研究

本研究通过对1982年黑龙江某鹅场发生的鹅细小病毒病疑似病例的肝病变组织病毒分离,进行回顾性研究.将肝脏加PBS研磨后,-70℃/37℃反复冻融三次,除菌过滤后接种12日龄鹅胚.发现病毒对鹅胚的致死时间为5～6 d,胚体体表有轻微出血点.鹅胚尿囊液经磷钨酸染色,可见具有典型特征的细小病毒粒子.将该病毒尿囊液在12日龄鹅胚中连续传16代,随着传代次数的增加,胚体多集中在3～4 d死亡.病毒传至第三代时死亡胚体头、颈、背部出现较为严重的出血点,体表有水肿.第五代病毒尿囊液(命名为HG5/82)对12日龄鹅胚的ELD50为10-5.92/0.1 mL.将20只12日龄雏鹅分为三个试验组和一个阴性对照组,各组分别接种鹅胚尿囊液104.92ELDso、103.92ELD50、102.92ELD50及生理盐水,发现雏鹅接种HG5/82后4 d出现轻微腹泻,随后恢复正常.用套式PCR检测雏鹅肛拭子病毒的体外排放情况.发现三个试验组在接种病毒后1～20 d用套式PCR均可检测到病毒.对照组及试验组接种后20～56d没有检测到病毒.将套式PCR产物进行序列测定并于参考毒株进行比较,表明该序列具有GPV相应区域的共同分子特征,与GPV参考毒株B的相应序列同源性达93.6%.本研究结果表明,该分离毒株为典型的鹅细小病毒(Goose Parvovirus,GPV),对雏鹅的致病力较弱.     

A review of feline serum protein electrophoresis

A review of the current literature available on feline serum proteins is presented. Early studies concentrated on comparative aspects of species variations in the electrophoretic pattern. The feline electrophoretogram was divided into five basic regions: albumin, alpha-1, alpha-2, beta and gamma. Different subdivisions of these areas were recognized depending on the support medium used. Current papers have compared the relative migration distances of each globulin peak to the migration of albumin. This "Rf" value enables reliable peak identification. To date, no data exists identifying the individual proteins responsible for the peaks in the alpha and beta regions. The only feline globulin to be studied is haptolobin; however its precise location on the electrophoretic strip was not identified.     

Chylothorax associated with cryptococcal mediastinal granuloma in a cat

A 10-year-old neutered female cat had chylothorax, precaval syndrome, and a mediastinal granuloma resulting from infection with Cryptococcus neoformans. Diagnosis of a chylous effusion was made by cytologic examination of pleural fluid and by finding higher triglyceride levels in the effusion than in serum (825 vs. 64 mg/dl, respectively). Postmortem examination revealed cryptococcal organisms in the mediastinal granuloma, lungs, cerebral meninges, and connective tissues adjacent to the thyroid gland. Chylous effusion in a cat associated with cryptococcosis has not been reported previously. Cryptococcosis should be included in the differential diagnosis in chylous effusions in cats.     

Lipoprotein electrophoresis differentiation of chylous and nonchylous pleural effusions in dogs and cats and its correlation with pleural effusion triglyceride concentration

Pleural effusions from 23 dogs and 25 cats hospitalized between November 1986 and April 1987 at the Veterinary Hospital of the University of Pennsylvania (VHUP) were classified as chylous (chylomicrons present) or nonchylous (chylomicrons absent) by the presence or absence, respectively, of a chylomicron band on lipoprotein electrophoresis gels of the effusions. Triglyceride concentrations, cholesterol concentrations, and cholesterol/triglyceride (C/T) ratios were compared between the chylous and nonchylous groups in each species. Cholesterol concentrations were not significantly different between chylous and nonchylous effusions in both dogs and cats. Cholesterol/triglyceride ratios of less than 1 were present in all dogs and cats with chylous effusions; however, 12% of dogs with nonchylous effusions and 50% of cats with nonchylous effusions also had a C/T ratio less than 1. Triglyceride concentrations accurately classified all effusions as chylous or nonchylous in both dogs and cats. Pleural effusions with triglyceride concentrations greater than l00 mg/dl were chylous in all cases, whereas, effusions with triglyceride concentrations less than l00 mg/dl were nonchylous in all cases.