滨白鸡纯系及杂交组合的种群关系分析

本文采用中国科学院遗传研究所研制的Ａ、Ｂ、Ｃ三个系统１２个血型因子，分析滨白鸡七个纯系和五个杂交组合的种群关系。结果表明，１２个群体可分为五个类。第一类包括５个纯系（ＮＯ２、ＮＯ７、ＮＯ６、ＮＯ３、ＮＯ８）和２个杂交组合（５×２、７×８）．第二类包括５×６组合和ＮＯ５系。第三类是４×２组合。第四类是５×４组合。第五类是ＮＯ４系。不同类品系间遗传差异大，同一类品系间则小。实际配合力测定得到的４×２、５×４２、６×４２优秀杂交组合均有一定的理论基础。     

Interrelationships between herd management practices and udder health status using canonical correspondence analysis

The relationships between the herd-level distribution of udder health measures (traumatic, physical, functional and congestive udder disorders, clinical mastitis and somatic cell counts (SCC) greater than 400 × 10³, ml⁻¹) and farm (general hygiene, milking practices and housing system) and aggregated individual characteristics were studied by canonical correspondence analysis (CCA). This is a correspondence-analysis technique in which the axes representing disease patterns are expressed as linear combinations of potentially explanatory variables. These main axes can be drawn so that the distribution of diseases for combinations of the main-explanatory managerial variables can be visualized. The type of quarantine for newcomers, type of animal housing, presence of shelter at pasture, use of udder towel before milking, milk production and loss of body condition after calving, were strongly correlated with udder health status. Mastitis and physical udder disorders were associated with the combination of no introduction of replacement heifers and low loss of body condition after calving. Congestive disorders were more common on farms when no quarantine for newcomers was combined with high loss of body condition after calving. Higher clinical and subclinical mastitis incidence risks were associated with the combination of no udder towel, loose housing with high animal density and high milk production. In most cases, clinical mastitis and high SCC were explained by the same covariates but with contrasting levels of those covariates.     

国内外发展草地畜牧业的现状与发展趋势

草地畜牧业在我国畜牧业经济中的比重不断增加,草地畜牧业的发展是关系到我国牧民增收、牧区经济发展的大问题。文章综述了国内外发展草地畜牧业现状,指出了我国发展草地畜牧业取得的成绩及存在的问题;借鉴国外发展草地畜牧业的成功经验,预测了我国草地畜牧业的发展趋势。     

盐胁迫对5份无芒雀麦苗期生长和生理生化的影响及综合性评价

为了明确不同无芒雀麦苗期耐盐能力的强弱,本研究选择5份不同株型的无芒雀麦作为供试材料。根据新疆土壤盐碱成分设置中性盐(M盐)：NaCl∶Na₂SO₄∶Na₂CO₃=1∶4∶0和碱性盐(A盐)：NaCl∶Na₂SO₄∶Na₂CO₃=1∶1∶8两种处理,于三叶期每盆一次性浇灌2 L电导率(EC)为20 ms·cm^-1的盐处理液,CK浇灌等量自来水。处理30 d后测定生长指标、叶绿素荧光特性参数、丙二醛(MDA)和渗透调节物质含量,并通过相关性分析、主成分分析、隶属函数分析对13个指标进行分析评价。结果表明：除F_v/F_m和F_v/F_o外,盐胁迫显著影响其余11个指标,且5份材料间差异显著(P<0.05);5份无芒雀麦的株高、茎粗、分蘖数、地上生物量、根冠比、MDA和脯氨酸含量在不同盐胁迫下表现出不同的适应变...     

矮小型褐壳蛋鸡产蛋时间和间隔的统计分析

本文对８８０只纯系矮小型褐壳蛋鸡的产蛋时间进行了连续５天的观察,分析结果表明,矮小型褐壳蛋鸡的产蛋时间集中在上午１２时以前,并以９：００～１０：００期间产蛋最为密集,占全天产蛋的１９．５％。连产内第一枚蛋产出时间集中在上午（８９：３３％）,以７：００～９：００间产蛋最多（４９．７１％）;而最后一枚蛋产出时间集中在下午（７１．２０％）,以２：００～４：００间产蛋最多（３６．５８％）。矮小型褐壳蛋鸡连产内产蛋间隔范围为２２．３５～３１．１５小时,每枚蛋平均间隔高峰在２４小时（３６．０％）,群体平均产蛋间隔为２５．０８小时。相邻连产间间隔时间的变异范围很大,最短为３８．０小时,最长达１００．４５小时。     

中国荷斯坦牛乳蛋白分子遗传多态性和产奶性状相关性的研究

从北京两个主要牛场共采得１８７头中国荷斯坦奶牛的血样,提取基因组ＤＮＡ,通过ＰＣＲ－ＲＦＬＰ方法对Ｋａｐｐａ酪蛋白、Ｂｅｔａ乳球蛋白（βｌｇ）和Ａｌｐｈａ乳白蛋白（α－ｌａ）进行了基因型的鉴定,并结合产奶性状进行统计分析,结果表明,牛群中上述３种乳蛋白基因的基因频率分别为Ｋ－ｃｎＡ７９％,Ｋ－ｃａＢ２１％,β－ｌｇＡ４３％,β－ｌｇＢ５７％,α－ｌｇＢ１００％,Ｋ－ＣＮ和β－ｌｇ基因位眯对产奶量没     

黑龙江省东南部林区6种啮齿动物食性分析

为了准确掌握黑龙江省东南部地区对农林危害比较严重的啮齿动物的食性及食物量,以便更好地、有针对性地灭鼠,从而为林业部门预防鼠害提供科学依据,于2010年3月—2011年1月通过实地考察,对捕获的鼠进行解剖,并结合实验室喂养进行食物量测定。结果表明,种子和浆果是树栖型种类啮齿动物的主要食物,约占70%左右;而地栖型种类啮齿动物的食物存在明显的季节性和地域性差异。     

毛发皮质醇激素作为慢性应激评定指标的研究进展

将毛发皮质醇激素作为反映慢性应激程度的黄金标准具有前瞻性和创新性。毛发皮质醇激素不仅可用于检测及估计动物慢性应激程度,且有助于了解动物体生理病理过程,并及时制定行之有效的健康管理策略。血液、唾液、尿液及乳汁中皮质醇激素含量作为反映急性应激程度的模型,被用于检测和建立持续的皮质醇激素昼夜循环规律研究。而毛发作为一个很好的补充模型却可以回顾性地反映慢性应激程度。毛发皮质醇激素作为反映慢性应激的生化指标不仅具有采样应激小、检测灵敏、方便的优点,而且是可遗传性状(h²=0.31),可以作为下丘脑-垂体-肾上腺轴(HPA轴)活性标记,进而利用其解释遗传变异对慢性应激中HPA轴活性变化的影响程度。作者综述了皮质醇激素与动物体健康的关系,慢性应激下机体调节机理及遗传适应改变,慢性应激评定指标研究进展,毛发作为皮质醇激素研究模型的研究进展,以及毛发皮质醇激素作为反映慢性应激的生化指标的限制与未来面对的挑战。     

Random-effects linear model application to herd-level assessment of bovine hepatic trace mineral concentrations

To evaluate the utility of random-effects linear modeling for herd-level evaluation of trace mineral status, we performed a retrospective analysis of the results for trace mineral testing of bovine liver samples submitted to the Michigan State University Veterinary Diagnostic Laboratory between 2011 and 2017. Our aim was to examine random-effects models for their potential utility in improving interpretation with minimal sample numbers. The database consisted of 1,658 animals distributed among 121 herds. Minerals were assayed by inductively coupled plasma–mass spectroscopy, and included cobalt, copper, iron, molybdenum, manganese, selenium, and zinc. Intraclass correlation coefficients for each mineral were significantly different (p < 0.001) from zero and ranged from 0.38 for manganese to 0.82 for selenium, indicating that the strength of herd effects, which are presumably related to diet, vary greatly by mineral. Analysis of the distribution and standard errors of best linear unbiased predictor (BLUP) values suggested that testing 5–10 animals per herd could place herds within 10 percentile units across the population of herds with 70–95% confidence, the confidence level varying among minerals. Herd means were generally similar to BLUPs, suggesting that means could be reasonably compared to BLUPs with respect to the distributions reported here. However, caution in interpreting means relative to BLUPs should be exercised when animal numbers are small, the standard errors of the means are large, and/or the values are near the extremes of the distribution.     

1株犬副流感病毒的分离鉴定及生物学特性研究

试验旨在分离鉴定犬副流感病毒(canine parainfluenza virus,CPIV),并对其生物学特性进行研究。用Vero细胞接种感染CPIV阳性犬肺脏组织,盲传4代,收集72 h病毒液进行RT-PCR鉴定、电镜观察、血凝试验、热敏性试验、紫外照射试验及病毒一步生长曲线的测定,同时扩增N基因进行序列分析,并构建系统进化树。结果显示,试验成功从出现咳嗽、流鼻涕等呼吸系统疾病症状的病犬肺脏中分离出1株CPIV,命名为CPIV-BJ01;RT-PCR扩增结果发现,在534 bp处有特异性目的条带。病毒电镜观察发现,其超微结构呈圆形、有囊膜、直径在80～200 nm之间;血凝试验显示,病毒在4和37℃均能凝集1%猪红细胞,与报道的CPIV血凝特性一致;病毒对热敏感,长时间高温下病毒毒价会随之下降;紫外照射可使病毒在短时间内对细胞的感染性急剧下降。病毒一步生长曲线测定结果显示,在12～48 h病毒高速增殖,细胞培养液中病毒滴度急剧上升,之后趋于稳定。CPIV N基因序列与19株有代表性的副流感病毒N基因相比,其核苷酸序列同源性为97.1%～99.8%。遗传进化分析表明,CPIV-BJ01与PIV5 1168-1(登录号:KC237064.1)和PIV5 ZJQ 221(登录号:KX100034.1)位于同一分支上,亲缘关系较近。