Influence of Veterinary Care on the Urinary Corticoid: Creatinine Ratio in Dogs

Physical and emotional stresses are known to increase the production and secretion of glucocorticoids by the adrenal cortex in both humans and experimental animals. The urinary corticoid: creatinine (C:C) ratio is increasingly used as a measure of adrenocortical function. In this study we investigated whether a visit to a veterinary practice for vaccination, a visit to a referral clinic for orthopedic examination, or hospitalization in a referral clinic for 1.5 days resulted in increases of the urinary C: C ratio in pet dogs. In experiment 1, owners collected voided urine samples from 19 healthy pet dogs at specified times before and after taking the dogs to a veterinary practice for yearly vaccination. In experiment 2, 12 pet dogs were evaluated in a similar way before and after an orthopedic examination at a referral clinic. In experiment 3, 9 healthy pet dogs were hospitalized for 1.5 days and urine samples were collected before, during, and after this stay. Basal urinary C:C ratios in all experiments ranged from 0.8 to 8.3 × 10^-6. In experiment 1, the urinary C:C ratio after the visit to the veterinary practice ranged from 0.9 to 22.0 × 10^-6. Six dogs had a significantly increased urinary C:C ratio (responders), but in 5 of these dogs the ratio was ≤10 × 10^-6 In experiment 2. 8 of 12 dogs responded significantly with urinary C:C ratios ranging from 3.1 to 27.0 × 10^-6. In experiment 3, 8 of 9 dogs had significantly increased urinary C:C ratios, ranging from 2.4 to 24.0 × 10^-6, in some or all urine samples collected during hospitalization. In 4 dogs urinary C:C ratios 12 hours after hospitalization were still significantly higher than the initial values. Thus, a visit to a veterinary practice, an orthopedic examination in a referral clinic, and hospitalization can be considered stressful conditions for dogs. A large variation occurs in response, and in individual dogs the increases in urinary C:C ratios can exceed the cutoff level for the diagnosis of hyperadrenocorticism. Therefore, urine samples for measurement of the C: C ratio in the diagnosis of hyperadrenocorticism should be collected in the dog's home environment, to avoid the influence of stress on glucocorticoid secretion.     

基因突变检测技术进展

基因未知突变检测在生物学各研究领域占有突出的地位,特别是人类遗传性疾病的未知突变检测及诊断。本文综述了目前使用较多的基因未知突变检测技术的原理和应用方法,特别是直接测序法（ＤＳ）、错配的化学切割分析（ＣＣＭ）、单链构象多态分析（ＳＳＣＰ）、核糖核酸酶切法（ＲＮａｓｅ）,变性梯度凝胶电泳（ＤＧＧＥ）等的进展,综合评价了各种方法的优缺点,便于选择应用。     

大学公共数学类课程考核方案改革实践与探索——基于云南农业大学的分析

回顾了云南农业大学公共数学类课程的考核方案变革历程,分析了不同课程考核制度的利弊.为充分体现课程考核对教学工作,包括教学内容、教学方法、教材选取、教学管理、师资队伍建设等的检验、评价、导向、反馈和激励功能,必须重视和推进有利于学生的综合素质提高和创新精神形成的考核改革.借助计算机及网络技术,以“多次学习过程测试”为主要手段,构建数学类课程综合性全程考核模式,促进大学公共数学类课程教学质量的提高.     

基于Web ActiveX数据控件的网络虚拟实验室构建

唐末竟陵籍作家皮日休与广州籍显宦郑愚之间属于座主门弟子关系，但他们个性、人生观等多方面存在着一些差异，在一些细节问题上发生过一定的误会和摩擦，本应亲密而转为隔膜。从皮、郑关系的考察中，可以看出皮日休的辩才、急智、桀骜不驯的性格，唐末文人风尚也可藉此而见一斑。     

计算机等级考试与计算机基础课程教学改革的思考

全国计算机等级考试在高校中逐渐普及，通过对计算机等级考试和计算机基础教学的关系，以及计算机基础教学现状和存在的问题的分析，提出了适应计算机等级考试要求，加强计算机基础教学的一系列改革措施。     

木本油料基地建设良种化问题及对策

针对目前木本油料产业发展中良种化存在的现有种苗生产、经营现状不适应良种化发展的需要,良种基地规模小,品种单一,良种审定工作滞后,良种科研与生产脱节等问题,提出全面规划、突出重点,建设一批林木良种基地,加快乡土树种选优、收集、保存和利用,建立健全林木良种推广体系等对策与措施.     

高职院校考试二级管理模式探析

论述了明确二级管理权限的重要性,探析了考试二级管理中的成绩评价体系及新的考试模式的建立。     

水貂配种期精液检查技术的研究

为研究水貂的繁殖率,避免母貂空怀,2010年3月3~20日对大连名威貂业有限公司配种期种貂精液进行了检查,结果表明:2 933只种公貂中有103只无精或死精,占种公貂总数的3.51%。说明做好精液检查是提高水貂妊娠率和产仔率的关键。如果采用同一只公貂复配,可降低母貂空怀率17.55%,能有效提高水貂的繁殖率。     

计算机辅助考试系统初始界面的开发

数据库应用基础Visual FoxPr06.0不但具有实用性,而且具有开发性功能.本文介绍了计算机辅助考试系统初始界面的开发过程.     

应用型人才培养目标下高校考试模式的改革与实践

高等学校的考试是检验高校教学效果和人才培养质量的一个重要手段,探讨了高校传统考试模式存在的主要问题,并对基于应用型人才培养目标的普通高校考试模式进行了探索,论述了新型考试模式的实践成效及其发展。