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101.
C. HAMBLIN JS SALT SD GRAHAM K. HOPWOOD AM WADE-EVANS 《Australian veterinary journal》1998,76(9):622-629
Objective To study the clinical signs following bluetongue virus serotypes 1 and 3 infection in Poll Dorset sheep.
Design A clinical and pathological study.
Procedure Twenty Poll Dorset sheep were inoculated with bluetongue virus serotypes 1 or 3, each inoculum having a different passage history. The sheep were examined daily and their clinical appearance and rectal temperatures recorded. Heparinised and non-heparinised blood samples were taken at intervals for virological and serological study. Gross pathological findings were recorded for several sheep at necropsy and tissue samples were collected from three sheep for virological studies.
Results All inoculated sheep developed clinical disease. The clinical signs and gross pathological changes varied considerably but were consistent with damage to the vascular endothelial system. There was a decline in the titres of infectious bluetongue virus and of antigen in tissues collected between 7 and 12 days after infection.
Conclusions The severity of disease was related to the speed of onset and duration of pyrexia and not the development or titre of viraemia. Generally, those animals with sensitive mouths, depression, coronitis, recumbency and reluctance to move were the most debilitated. Whole blood was the most reliable source of infectious virus from acutely and chronically infected and convalescent animals. However, tissue samples particularly spleen, collected from dead or killed animals suffering from either peracute or acute forms of disease were most appropriate for the rapid confirmation of a clinical diagnosis. 相似文献
Design A clinical and pathological study.
Procedure Twenty Poll Dorset sheep were inoculated with bluetongue virus serotypes 1 or 3, each inoculum having a different passage history. The sheep were examined daily and their clinical appearance and rectal temperatures recorded. Heparinised and non-heparinised blood samples were taken at intervals for virological and serological study. Gross pathological findings were recorded for several sheep at necropsy and tissue samples were collected from three sheep for virological studies.
Results All inoculated sheep developed clinical disease. The clinical signs and gross pathological changes varied considerably but were consistent with damage to the vascular endothelial system. There was a decline in the titres of infectious bluetongue virus and of antigen in tissues collected between 7 and 12 days after infection.
Conclusions The severity of disease was related to the speed of onset and duration of pyrexia and not the development or titre of viraemia. Generally, those animals with sensitive mouths, depression, coronitis, recumbency and reluctance to move were the most debilitated. Whole blood was the most reliable source of infectious virus from acutely and chronically infected and convalescent animals. However, tissue samples particularly spleen, collected from dead or killed animals suffering from either peracute or acute forms of disease were most appropriate for the rapid confirmation of a clinical diagnosis. 相似文献
102.
1日龄父母代罗曼(Lohmann)蛋鸡120只,随机分为3组,分别喂以低锰(基础日粮含锰13.62mg/kg)、低锰高磷(基础日粮+0.5%的磷)、补锰对照(基础日粮+锰80mg/kg)日粮。试验期8周。饲喂5~8周发病。低锰组发病率26%,低锰高磷组28%,补锰对照组无一发病。第4周时,体重分别为(267.9±8.32)、(214.2±10.42)、(265.0±12.18)g,低锰组、补锰对照组与低锰高磷组之间差异极显著(P<0.01)。肝锰含量分别为(5.40±0.74)、(2.82±0.36)、(8.48±0.96)mg/kg,补锰对照组与低锰组差异显著(P<0.05),与低锰高磷组差异极显著(P<0.01)。发病症状:精神沉郁,不愿走动,伏卧,跛行,单腿发病病腿向外侧伸展,双腿发病则内收呈“O”字形或外展呈“八”字形。剖检跗关节肿大,胫骨髁骨骨质增生、肿大,髁间沟变平坦。组织学检查,胫骨骺生长板增殖区变窄,软骨基质中粘多糖减少。超微结构变化,心肌纤维、肝细胞、胫骨骺生长板软骨细胞、睾丸精原细胞等线粒体肿胀与空泡化。 相似文献
103.
鸡实验性尿酸盐沉积症的肾脏病理学研究 总被引:5,自引:2,他引:3
将120只35日龄迪卡商品代蛋用雏鸡随机分为高钙组、高蛋白组和对照组,分别饲喂高钙(含钙3.9%)、高蛋白(含粗蛋白44.0%)和全价(含钙0.8%,粗蛋白18.2%)饲料。试验第25天,各组剖检5只鸡,作肾脏的光镜和电镜观察。结果表明,高钙、高蛋白饲料均能引发尿酸盐沉积。高钙组鸡肾脏肿大,表面及切面有大量尿酸盐结晶;输尿管扩张,管内充满白色尿酸盐沉积物。光镜观察,肾小球肿胀,近曲及远曲小管上皮细胞肿胀、变性。电镜观察,近曲小管上皮绒毛短缩、肿胀、断裂、脱落,线粒体肿胀、嵴断裂;远曲小管线粒体肿胀,呈现空泡,核呈锯齿型。高蛋白组眼观病变不明显,肾脏的光镜、电镜病变与高钙组相似,但损伤程度较轻。作者认为,高钙、高蛋白所致肾脏损害是鸡尿酸盐沉积症的重要原因。 相似文献
104.
Ultrasonography of the femoral artery in the horse, including color, power and spectral Doppler, provides morphologic and dynamic information. This paper describes the use of the techniques in six clinically normal horses and three with femoral artery thrombosis. Useful landmarks for orientation are the saphenous artery and the medial saphenous vein. The lateral circumflex femoral artery can not be visualized. Recognition of the genus descendens artery is complicated due to the presence of multiple distal caudal femoral arteries. The femoral artery feeds a high resistance bed. In normal horses the peak systolic velocity varies between 50–90 cm/sec. Echoic tissue in the femoral artery lumen with stenosis, occlusion and collateral blood flow formation are features encountered in the patients. 相似文献
105.
马传染性贫血病毒弱毒株LTR的克隆及序列分析 总被引:9,自引:0,他引:9
从马传染性贫血病毒(EIAV)弱毒株感染的驴胎皮肤(FDD)细胞中提取前病毒DNA,以其为模板,通过PCR扩增出EIAV弱毒株的LTR,并将其克隆到载体质粒pUC19中。经酶切分析,PCR及Southern杂交筛选、鉴定,获得含有EIAV弱毒株LTR片段的重组质粒pLTR。对该质粒的序列分析发现,在中国EIAV弱毒株LTR的U3区含有4个与细胞转录因子结合的位点,其中有3个PU.1位点和1个AP-1位点,U3区还存在1个TATATAA启动子序列;R区长为81bp,含有1个帽位点GG和1个Poly(A)信号序列AATAAA;在帽位点下游是1个病毒Tat蛋白结合位点,即TAR位点;U5区长为39bp。中国EIAV弱毒株LTR序列与国外发表的其他毒株序列相比,在LTR的一些调控部位有变异发生,中国EIAV弱毒株与美国EIAV原型株(Wyoming株)LTR区核苷酸序列有18.72%的差异。 相似文献
106.
用自然感染马阳性血清及兔免疫血清制备的马鼻肺炎荧光抗体(EHV_1·FA),和马鼻肺炎病毒(HEV_1)不同毒株以及从匈牙利引进的Bartha株标准毒均产生特异性荧光。通过对不同滴度地鼠肾细胞培养液内抗原的测定,表明EHV_1·FA法比补结反应及细胞病变法敏感,高1~2个滴度,应用冰冻切片直接荧光染色检查乳地鼠31份,肝阳性率为83.8%,肺阳性率为77.4%,对照10份均为阴性。检查人工感染HEV_1流产胎儿5份,肝阳性率为100%,肺阳性为80%,检查2份健康驴胎儿,均为阴性。表明应用冰冻切片、直接荧光染色检查马鼻肺炎病毒具有较高特异性和检出率。 相似文献
107.
JONATHAN L. STOWATER DVM MS CARL A. KIRKER-HEAD MA VET MB MRCVS RICHARD M. JAKOWSKI DVM PHD 《Veterinary radiology & ultrasound》1986,27(4):115-117
Osteochondrosis of the lateral femoral condyles was diagnosed radiographically in an 8-month-old, female Arabian horse, which had been presented with a hindlimb lameness. The diagnosis was confirmed by gross and microscopic pathology. The location of the lesions was considered unusual for osteochondrosis in the horse. 相似文献
108.
松材线虫侵染对松树苯丙氨酸解氨酶及酚类物质的影响 总被引:2,自引:0,他引:2
以湿地松(Pinus elliottii Engelm)、日本黑松(Pinus thunbergii Parl)为材料,研究松材线虫[Bursaphelenchus xylophilus(Steiner&Buhrer,1934)Nickle]感染对苯丙氨酸解氨酶和酚类物质的影响.两种松树的苯丙氨酸解氨酶的活性在发病过程中都有明显增加,并且伴随着酶活性的变化,各种苯丙烷次生代谢产物,如总酚、类黄酮的含量也发生明显的变化.但湿地松在苯丙氨酸解氨酶活力和酚类物质的积累上的变化比黑松要大些.结果表明,松材线虫病的发生、发展与寄主植物苯丙氨酸解氨酶活性和酚类物质含量变化有关. 相似文献
109.
110.
GROSS AND HISTOPATHOLOGIC CORRELATION OF LOW‐FIELD MAGNETIC RESONANCE IMAGING FINDINGS IN THE STIFLE OF ASYMPTOMATIC HORSES 下载免费PDF全文
Marcos P. Santos Santiago D. Gutierrez‐Nibeyro Alexia L. McKnight Kuldeep Singh 《Veterinary radiology & ultrasound》2015,56(4):407-416
With the recent introduction of a 0.25T rotating MRI system, clinical evaluation of the equine stifle joint is now possible in the average equine athlete. A recent publication described common abnormalities of horses with stifle lameness detected with a low‐field MRI system; however, postmortem corroboration of the lesions detected was not possible. Therefore, our objective was to compare postmortem findings with low‐field MRI findings in equine cadaver stifle joints. Ten fresh cadaver stifle joints from horses without clinical signs of stifle disease were evaluated using low‐field MRI, gross dissection, and histopathology. In eight stifles, either the lateral or medial cranial meniscotibial ligament had an irregular shape, fiber separation, or moderate abnormal signal intensity (SI) on all sequences. In five stifles, the medial femoral condyle had articular cartilage fibrillation with or without an osteochondral defect over the weight bearing surface of the medial femoral condyle. All stifles had abnormal SI on all sequences within the patellar ligaments that corresponded with adipose tissue infiltrating between the collagen bundles. Other abnormalities identified included articular cartilage fibrillation of the tibial condyles in three stifles, and articular cartilage fibrillation with chondral defects in the patella in three stifles. All abnormalities detected with low‐field MRI were corroborated by gross dissection. Findings from the current study supported the use of low‐field MRI for detection of stifle joint lesions in horses and demonstrated that some stifle joint pathologies may be subclinical in horses. 相似文献