我国数字图书馆发展现状

对我国数字图书馆的初始阶段，发展阶段和应用阶段做了简要的阐述，并介绍了一些图书馆和教育部的数字化工程。     

以图书馆搬迁为契机重塑高校图书馆整体形象

文章结合我馆搬迁的实际工作,论述了以科学规划为指导的图书馆搬迁推动了高校图书馆服务方式扣管理模式的改进,更新思雏方法,树立以人为本的服务理念,重新塑造高校图书馆的职业道德形象、学术形象、优质服务形象、内部环境形象等整体形象。     

基层图书馆为弱势群体服务的现状与思考——以徐州地区为例

在分析弱势群体构成和文化需求的基础上,以徐州地区为例论述了基层图书馆为农村弱势群体服务的若干举措和实践活动,探讨为农村弱势群体服务的方式方法,并对今后工作提出具体建议。     

图书馆馆藏数据中存在的问题及对策

馆藏数据是读者了解馆藏文献资源的窗口,规范、优化馆藏数据,进而提高馆藏数据的质量是图书馆为读者提供满意服务的有力保障。结合图书馆馆藏数据维护工作实际,分析了馆藏数据中存在的问题,提出了提高馆藏数据质量的应对策略。     

高校图书馆开展网络“图书漂流”服务的平台选择和模式分析

阐述了高校图书馆“图书漂流”服务的开展现状及存在的问题,并对网络“图书漂流”服务模式的主要平台进行调查和对比分析,结合高校图书馆的特殊环境和特点,探索一种网站结合微信公众平台的“图书漂流”服务模式的开展,并对平台结合模式进行了具体分析。     

Lib2.0下开展图书馆学科化服务

Lib2.0的理念和方法引入高校图书馆,有利于提高图书馆的创新服务,开展学科化服务对提升图书馆服务水平、保障图书馆资源利用的最大化等有重要价值。     

图书馆建筑设计的原则

文章通过对现代图书馆建筑特点的分析研究,阐述现代图书馆对建筑的要求及现代图书馆建筑设计应遵循的基本原则。     

Preparation of high molecular weight （HMW） genomic DNA from tea plant （Camellia sinensis） for BAC library construction

A bacterial artificial chromosome （BAC） library is an invaluable resource tool to initiate tea plant genomics research, and the preparation of high molecular weight （HMW） genomic DNA is a crucial first step for constructing a BAC Library. In order to construct a BAC library for enhancing tea plant genomics research, a new method for the preparation of tea pant high molecular weight （HMW） genomic DNA must be developed due to young tea plant leaves and shoots are notably rich in both tea polyphenols and tea polysaccharides. In this paper, a modified method for preparing high quality tea plant HMW genomi~ DNA was optimized, and the quality of tea plant genomic DNA was evaluated. The results were as follows： Critical indicators of HMW DNA preparation were the appearance of the smooth nuclei in solution （as opposed to sticky-gummy） before agarose plug solidification, non-dark colored nuclei plugs after lysis with an SDS/proteinase K solution, and the quality and quantity of HMW DNA fragments after restriction enzyme digestion. Importantly, 1% dissolved PVP-40 and 1% un-dissolved PVP-40 during the nuclei extraction steps, in conjunction with the removal of PVP-40 from the plug washing and nuclei lysis steps, were critical for achieving HWM tea plant DNA suitable for BAC library construction. Additionally, a third PFGE fraction selection step to eliminate contaminating small DNA fragments. The modifications provided parameters that may have prevented deleterious interactions from tea polyphenols and tea polysaccharides. The HMW genomic DNA produced by this new modified method has been used to successfully construct a large-insert tea plant BAC library, and thus may be suitable for BAC library construction from other plant species that contain similarly interfering compounds.     

高校图书馆文献资源建设的九项原则

我国高校图书馆文献资源建设工作受文献剧增、读者需求扩大、经费短缺等问题困绕,应坚持针对性、特色化、整体性和发展性的基本建设原则,注重成本效益和资源优化配置,以集团购买、共建共享方式,扬长避短建立切实有效的文献资源保障体系。