拖拉机田间作业节能对比试验方法

随着世界能源危机问题日益突出,拖拉机工业面临着严峻的挑战.为此,根据拖拉机运用学的理论提出了拖拉机田间作业燃油经济性的评价指标,阐述了拖拉机田间作业节能对比的试验方法,并在实践中得以验证,旨在为评价拖拉机田间作业的燃油经济性提供依据.     

微机交流采样系统的研制

交流电量测量是水电站自动化中的基本环节。文章作者在湖南省嘉禾县调塘水电站实现微机监控系统中研制了基于8098单片机的交流采样,通过初步调试证明:用微机来直接采集交流电量可使系统结构得以简化,采集数据稳定性及精度均较高,便于现场调试,可以充分利用8098芯片的功能,满足中小型电站测量要求,具有实用价值。     

X195柴油机优化调整节能技术的研究

动力性和经济性是柴油机的两个重要指标，广大发动机研究者一直致力于改善这两个指标。特别是在能源短缺的今天，如何提高发动机的动力性和经济性的问题就显得尤为重要。通过大量的台架实验．研究了X195柴油机的3项主要的调整参数(供油提前角、喷油压力、气门间隙)对其动力性和经济性的影响，分析了其影响因素，分别找出了最佳的调整范围。     

摄食水平对黄颡鱼氮和能量收支的影响

在25℃、自然光照条件下,测定黄颡鱼在摄食水平为1/3饱食、2/3饱食和饱食时的氮和能量收支.结果显示,摄食水平对黄颡鱼的氮收支和能量收支均有显著影响,随着摄食水平的提高,生长氮和生长能的比例显著提高,排泄氮、粪氮和代谢能的比例显著下降,但在饱食2/3和饱食水平时,生长氮、生长能、排泄氮和代谢能比例差异都不显著.     

德国可再生能源发展的借鉴与启示

近年来,可再生能源逐渐成为世界各国关注的热点,并成为各国应对气候变化以及生态环境保护的共同选择,其在经济发展以及维护生态环境中开始被重视起来.德国能源转型的成功已经成为欧美国家中的典范,为此农业农村部科技教育司组团赴德国参加"农业废弃物资源化利用和农村清洁能源综合利用技术"培训项目,笔者结合培训内容和实地考察形成该篇文...     

湘潭市能源的生态足迹及森林固碳减排效应的分析

生态足迹作为可持续发展定量研究的一种新方法,具有简明而综合的特点。采用生态足迹成分法对湘潭市2003年能源的生态足迹进行了计算,并对湘潭市2003年CO2排放量及森林固碳减排效应进行了分析。结果表明:2003年湘潭市人均能源生态足迹为0.653 hm2,实有人均能源生态足迹为0.077 hm2,人均能源生态足迹赤字为0.576hm2;2003年湘潭市CO2排放量为1 186.42万t,现有森林CO2年吸收量为140.25万t。通过扩大森林面积和提高森林生产力,每年可多吸收74.27万t CO2,实现减排6.26%。     

赤眼鳟和麦瑞加拉鲮的化学组成、能量密度及对鳜的营养价值

为评价赤眼鳟(Squaliobarbus curriculus)和麦瑞加拉鲮(Cirrhinus mrigala)对鳜(Siniperca chuatsi)的营养价值,分别测定了体质量为(5.27±1.03)g的赤眼鳟、(5.05±1.21)g的麦瑞加拉鲮和(5.19±0.92)g的鳜的化学组成,估算了这3种鱼的能量密度,以探讨赤眼鳟代替麦瑞加拉鲮养殖鳜的可行性。结果显示,赤眼鳟和麦瑞加拉鲮的全鱼蛋白质含量显著高于鳜,水分显著低于鳜(P0.05),必需氨基酸指数分别高达92.56%±2.31%和93.17%±0.36%,多数必需氨基酸的化学评分≥0.82。而赤眼鳟的脂肪和能量密度更高,显著高于麦瑞加拉鲮和鳜,灰分显著低于麦瑞加拉鲮(P0.05)。研究表明,这两种鱼对鳜都具有较高的营养价值,但赤眼鳟的营养价值更高,以赤眼鳟代替麦瑞加拉鲮养殖鳜是可行的,这为转变鳜的养殖方式提供了参考。     

扇吸式太阳能杀虫灯在鲜食玉米田的应用效果及对节肢动物生物多样性的影响

为明确扇吸式杀虫灯在鲜食玉米田间的应用效果及对节肢动物生物多样性的影响,采用抽样鉴定方法调查扇吸式杀虫灯的诱虫谱,通过田间试验调查其对主要害虫亚洲玉米螟Ostrinia furnacalis的控制范围和防控效果,并采用黄板、糖醋酒液和陷阱法取样调查其对玉米田间节肢动物生物多样性的影响。结果表明,扇吸式杀虫灯在鲜食玉米田可诱集9目40科的昆虫,对亚洲玉米螟的有效控制距离在60 m左右,单灯控制面积约1.1 hm²。在大喇叭口期,灯光防控处理对亚洲玉米螟的株防效为25.83%,显著低于化学防控处理的76.74%;在乳熟期,化学防控处理的虫孔减退率为75.29%,显著高于灯光防控处理的21.18%,灯光防控处理、化学防控处理和对照的茎秆活虫数、虫孔隧道长度和雌穗防效差异均不显著。距灯80 m范围内不同距离的主要害虫与天敌种群数量基本一致,玉米不同生育期节肢动物群落多样性指数、均匀性指数、优势集中性指数和相似系数变化较大,但在同一生育期不同距离间均无显著差异,说明田间节肢动物群落主要受玉米生育期的影响,扇吸式杀虫灯对田间主要害虫和天敌种群无显著影响。表明扇吸式杀虫灯对亚洲玉米螟的防治效果有限,对田间节肢动物多样性无显著影响,可作为鲜食玉米田防治亚洲玉米螟的辅助手段,但无法替代化学防治。     

Effects of different active constituents of Gynostemma pentaphyllum on mitochondrial energy metabolism-related proteins in endothelial cells induced by ox-LDL

AIM To investigate the effects of different components of Gynostemma pentaphyllum ［gypenosides (Gps), gypenoside XLIX (GpXLIX) and ginsenoside Rb3 (GRb3)］ on mitochondrial energy metabolism-related proteins in endothelial cells induced by oxidized low-density lipoprotein (ox-LDL). METHODS EA.hy926 cells were divided into control group, model group, Gps group, GpXLIX group and GRb3 group. The cells in control group were cultured only in DMEM complete medium. The cells in model group were treated with 100 mg/L ox-LDL for 48 h. The cells in Gps group, GpXLIX group and GRb3 group were treated with 100 mg/L ox-LDL for 24 h, and then treated with Gps, GpXLIX and GRb3 at 100 mg/L for another 24 h, respectively. The ATP content in each group was detected by ELISA. The expression levels of mitochondrial energy metabolism-related proteins, cytochrome C oxidase subunit 5a （Cox5a）, NADH:ubiquinone oxidoreductase core subunit S1 （Ndufs1）, ATP synthase F1 subunit alpha (ATP5a) and cytochrome C (Cyt C）, were determined by Wes automatic Western blot quantitative analysis system and Western blot. RESULTS Compared with control group, the ATP content in model group was decreased (P<0.01). After drug intervention, the ATP content increased to different degrees in Gps group, GpXLIX group and GRb3 group (P<0.01). The results of Wes automatic Western blot quantitative analysis system were consistent with those of Western blot. These results showed that compared with control group, the protein expression of Cox5a, Ndufs1 and ATP5a in model group was decreased, and the protein expression of Cyt C was increased (P<0.01). After intervention, the protein expression of Cox5a, Ndufs1 and ATP5a was increased and the protein expression of Cyt C was decreased in Gps group, GpXLIX group and GRb3 group (P<0.05 or P<0.01). Among them, the effect of Gps on the protein expression of Cox5a, Ndufs1 and Cyt C was significantly stronger than those of the 2 monomer components, and the effect of GRb3 was found to be superior in the 2 monomer components. The effect of GpXLIX on ATP5a protein was superior to the other 2 components. CONCLUSION Gynostemma total saponins and related active ingredients protect ox-LDL-induced endothelial cells by affecting mitochondrial energy metabolism-related proteins, thereby preventing and treating atherosclerosis.