中华补血草和德国补血草的耐盐性比较

【目的】比较中华补血草(Limonium sinense Kuntze)和德国补血草（Limonnium tataricum）的耐盐能力。【方法】以中华补血草和德国补血草为试材,设置1.66 （对照CK）,4,6,8,10,12,14 mg/g NaCl胁迫处理,分析不同处理下2种补血草的生长情况及叶片中丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性和电解质外渗率等指标变化,并比较2种补血草叶片的盐腺分布特征。【结果】在1.66～8 mg/g NaCl胁迫下,2种补血草植株外部形态无明显变化;在10～14 mg/g NaCl胁迫下,随盐胁迫程度的加剧,2种补血草盐害症状加重,且德国补血草比中华补血草盐害程度深,出现盐害症状时间早。在 1.66～10 mg/g NaCl胁迫下,2种补血草叶片中的MDA含量变化小,德国补血草在NaCl含量为12 mg/g时、中华补血草在NaCl含量为14 mg/g时,MDA含量上升;SOD活性均随NaCl含量的增加而先升后降,中华补血草增幅大,而德国补血草降幅大;2种补血草细胞电解质外渗率均随NaCl含量的增加而总体呈上升趋势,德国补血草上升幅度大。中华补血草叶表皮分布的盐腺直径极显著小于德国补血草,分布密度极显著大于德国补血草,盐腺面积所占比例较德国补血草大。【结论】中华补血草能耐NaCl含量为14 mg/g的土壤,德国补血草能耐NaCl含量为12 mg/g的土壤,中华补血草的耐盐性强于德国补血草。     

Are Primary Cultures of Trout (<Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>) Ventricular Cardiomyocytes Metabolically Viable?

The objectives of this study were to establish a protocol for the isolation of metabolically viable ventricular cardiomyocytes from the rainbow trout and to determine which measures may best reflect viability 24 h after isolation. Cardiomyocytes were isolated by enzymatic digestion and maintained in cell suspension. Viability was assessed using Trypan blue dye exclusion, ATP content, oxygen consumption and lactate dehydrogenase (LDH) leakage into the medium. Viability, assessed by these measures did not significantly change over the time period of this study. ATP content did correlate significantly with oxygen consumption but not with Trypan blue exclusion. We conclude that primary cultured cardiomyocytes remain metabolically viable for at least 24 h after isolation. Also, it appears that ATP content and oxygen consumption most adequately reflect metabolic cell viability. To be confident with a culture, however, a combination of viability measures is necessary when isolating cardiomyocytes from fish.     

Improving the Field Emergence Performance of Super Sweet Corn by Sand Priming

Abstract

A priming method called sand priming was developed using sand as a priming solid matrix. The effect of sand priming on improving the field emergence performance of five super sweet corn cultivars was investigated. Sand priming significantly improved field emergence performance of all super sweet corn cultivars, and there was marked improvement by priming at 20ºC for 24 hr. After sand priming at 20ºC for 24 hr, field emergence percentage (FEP) of “Green Superman”, “Huatian 1”, “Yangtian 1”, “Mitian 8”, and “Chaotian 43” was increased by 52.1%, 37.5%, 38.0%, 40.9%, and 33.3%, respectively. Their field emergence speed (FES) was 2.3, 1.8, 2.0, 2.0, and 1.8 times of the control, respectively. To further elucidate the effect of sand priming on improving the field emergence performance of super sweet corn, we analyzed the membrane system integrity, α-amylase activity and protein content. Sand priming at 20ºC for 24 hr improved membrane system integrity and α-amylase activity in all super sweet corn cultivars. Furthermore, sand priming at 20ºC for 24 hr accelerated the degradation of embryo protein after 1 d germination in “Green Superman”.     

二极管反向漏电流特性测试仪的设计

设计了一款在0~100℃和反向电压VR为0~1 000 V条件下,能够测试二极管反向漏电流特性的测试仪.它采用桥式输入I、CL7650组成的高精度放大电路、ADC0804和AT89S51完成了高压到低压的转换、信号的放大、采样、量化及显示,通过编程软件对测试结果进行修正,提高系统的测量精确度.对二极管IN4007的测试结果表明:所得反向漏电流分辨率达0.1 nA,准确度达1.79%.     

浅谈外墙渗漏

首先从诸多方面分析了外墙渗漏的原因,然后论述了具体的防治渗漏措施并介绍了可使用的两种新材料。     

钻孔灌注桩施工中岩层漏浆故障处理

本文通过对某大桥钻孔灌注桩施工情况分析。阐述了钻孔灌注桩施工中岩层漏浆故障的原因。同时介绍了几种处理方法。如补浆法、封堵法、内护筒法。     

船用钢板漏磁检测的三维有限元分析

为保证船舶的安全,满足对船用钢板进行定期检测的需要,提出利用漏磁检测钢板缺陷的方法,并对该方法进行了三维有限元分析和实验验证.在ANSYS软件中建立检测传感系统、磁化装置和钢板缺陷的三维模型,进行钢板缺陷附近的磁场分布状态的数值仿真,得出了船用钢板磁化后的磁场分布状态,计算出缺陷与磁场分布之间的对应关系曲线,并对缺陷进行判断.检测实验结果证明有限元计算是可靠的.     

硬盘数据的安全防御

随着信息和网络技术的快速发展,越来越多的重要数据存储在硬盘上,硬盘数据的安全保护逐渐成为备受关注的问题。通过对计算机硬盘数据结构的分析,结合人们对计算机硬盘数据安全的认识误区,全面阐述了威胁计算机硬盘数据安全的几个因素及其防范措施。     

一种油罐底板实时监漏的新方法

针对当前被广泛使用的通过罐内油位变化和感官查库对油罐底板进行实时监漏的方法存在的弊端,提出了油罐底板实时监漏的新方法,即油罐基础内钻孔油气体积分数监漏法.介绍了油罐底板实时监漏方法的探漏原理:在油罐基础上按每9m(弧长)钻取一个直达砂垫层的探漏孔,使用高灵敏度的油气含量检测仪探测到油气,可判断罐底是否发生渗漏.以实例阐述了操作步骤和现场应用情况,证明了其有效性,详述了其技术要求.基于现场实验数据,提出了汽油油罐的判漏标准,可为今后油库的扩容和改造提供借鉴.     

一种流动体泄漏自动检测新方法

流动体泄漏自动检测系统是基于通信传输理论，根据流动体泄漏的特点，提出了一种全新的泄漏检测方法。该方法采用在流动体发送端加入测试物质或测试信号，在接收端检测该物质或信号，依据其变化确定是否有泄漏。