Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.     

Immunogenicity of multiple-epitope antigen gene of HCV carried by novel biodegradable polymers

In order to develop a promising vaccine candidate utilizing a combined approach to induce both antibody production and T-cell activity, the DNA fragment containing MA of HCV with five conserved epitopes was synthesized. Two types of HCV vaccine candidates (the DNA type and DNA/polymers) were constructed using MA. PLA-PEG-PLA and PLGA-PEG-PLGA were synthesized and used as micelles with encapsulated plasmid pcDNA3.1(+)-MA. The preparation of copolymers, the cloning and analysis of recombinant plasmid DNA, in vitro expression, and immunogenicity in transgenic mice were evaluated in detail. The results indicated that even single immunization and oral immunization with DNA/polymers achieved satisfying immune responses in vivo tests. As biodegradable and nontoxic triblock copolymers, the novel copolymers demonstrated a great advantage, as they made long-term and single-immunizing vaccines possible; in addition, the copolymers showed a better adjuvant effect and scarcely any side effects.     

基于层次分析法的食用菌电商平台营销系统的改进模型

针对食用菌电商平台营销策略的改进问题,基于层次分析法,设计了食用菌电商平台营销策略评价指标体系,并结合模糊综合评价模型对该评价体系进行了定量和定性分析,构建了评价模型。该评价模型能够科学、全面地反映食用菌电商平台的营销能力和发展情况,对提高食用菌企业和电商平台的营销能力具有重要意义。     

《梅山续稿》中食(药)用菌意象解读

南宋诗人姜特立所著的《梅山续稿》是一本内容丰富的诗文集。与大多数诗人不同的是姜特立在诗中塑造了独特的食(药)用菌意象。"意象"塑造是中国古典诗歌独特的审美追求。通过对诗中"意象"的解读,可以准确把握诗人创作倾向,思想所指和情感蕴含。选择《香菌诗》、《子陵瀬》、《人送岩桂之二》、《感物》4首具有代表性的诗歌进行研究和分析,对诗中食(药)用菌意象进行解读。探讨诗中的意象内蕴,挖掘诗歌的深刻内涵,以丰富我车优秀的诗歌文化。     

基于协同理论产业融合的食用菌电子商务系统构建

基于企业战略协同理论,对食用菌企业协同战略的必要性进行了讨论,介绍了几种食用菌电子商务的模式。在竞争日益激烈的电子商务领域,食用菌行业的电子商务模式也需要战略上的协同与合作,企业在竞争与合作之间要张弛有度、合理控制彼此间的关系,保持自身的核心竞争力,实现合作共赢。     

基于层次分析法的食用菌产业扶贫绩效评价

特色产业扶贫是一种创新式的扶贫模式,通过扶持贫困地区发展地方特色产业脱贫致富。食用菌营养丰富,销售市场前景乐观,也响应了我国绿色环保节能的理念,成为最具优势的产业发展趋势。辽宁省某地区作为我国脱贫攻坚战的重点地区,利用食用菌产业不断优化自身的农业结构,助力当地脱贫致富。对该地区的食用菌产业扶贫绩效进行了具体分析和评价。     

食用菌产业助力脱贫攻坚与乡村振兴实证分析

以连云港市食用菌产业推动连云港经济发展为例,阐明食用菌产业助力乡村脱贫攻坚和农民致富的显著效果。食用菌产业的发展促进了当地农业增产增效,带动了农民增收致富,凸显了其重要的社会经济效益和生态效益。在政府扶贫支持的带动下,发展食用菌产业助力脱贫攻坚与乡村振兴已在连云港市基本实现。     

传统图形元素在食用菌包装创意设计中的运用

通过研究食用菌包装中传统图形元素的创意设计,可以使食用菌产品与现代消费者的需求更加贴合,进一步拓展食用菌消费市场。融合地域特色传统文化,满足现代人的审美观念,对传统图形元素进行有效创新,并使用科学的方法进行设计,更好的凸显地域特色文化内涵。     

食用菌产业发展对农业循环经济的影响

针对我国食用菌产业发展对农业循环经济的影响问题,提出了食用菌产业转变生产方式、实施专业化分工协作,以节约经济运行成本;在分析成本效益的基础上,建立了食用菌产业发展的影响因素评价体系,对农业循环经济发展过程中的多种复杂影响因素进行科学评价,重点评价了经济发展的可持续性、经济性和环保性三个影响因素。该评价体系对我国食用菌产业发展对农业循环经济的影响因素做出科学的评价,能够辅助相关政府部门进行经济决策。     

互联网订单模式与食用菌产业融合的可行性发展

系统阐述了互联网订单生产模式在我国食用菌产业中实施的可行性。首先从我国目前食用菌产业的低成本生产现状分析了订单生产的战略可行性;其次从食用菌产业的国际环境和互联网科技的发展方面分析了发展订单式生产的有利因素,提出了创新食用菌产业订单模式的一些建议。指出了巩固订单模式实践效果应注意的若干问题。对促进我国食用菌产业创新发展模式提供了借鉴。