小麦平衡施用氮磷钾肥经济效益的研究

采用“3414”回归最优设计原理设置小麦肥效试验,根据试验结果获得肥料效应函数方程,由此数学模型得出理论氮、磷、钾最佳施肥量分别为N184．5kg／hm^2、P2O5 78．0kg／hm^2、K2O 58．5kg／hm^2,最佳产量7021．5kg／hm^2。综合分析结合当地农业生产实际,潮土类两合土的氮、磷、钾建议施肥量分别为180～225kg／hm^2、75～90kg／hm^2和60～75kg／hm^2。     

Clinical applications of adipose-derived stromal vascular fraction in veterinary practice

Adipose tissue-derived stromal vascular fraction (AdSVF) comprises a heterogeneous cell population, including the multipotent mesenchymal stem cells, hematopoietic stem cells, immune cells, endothelial cells, fibroblasts, and pericytes. As such, multipotent adipose tissue-derived mesenchymal stem cells (AdMSCs), are one of the important components of AdSVF. Commonly used techniques to harvest AdSVF involve enzymatic or non-enzymatic methods. The enzymatic method is considered to be the gold standard technique due to its higher yield. The cellular components of AdSVF can be resuspended in normal saline, platelet-rich plasma, or phosphate-buffered saline to produce a ready-to-use solution. Freshly isolated AdSVF has exhibited promising osteogenic and vasculogenic capacity. AdSVF has already been proven to possess therapeutic potential for osteoarthritis management. It is also an attractive therapeutic option for enhancing wound healing. In addition, the combined use of AdSVF and platelet-rich plasma has an additive stimulatory effect in accelerating wound healing and can be considered an alternative to AdMSC treatment. It is also widely used for managing various orthopaedic conditions in clinical settings and has the potential for regenerating bone, cartilage, and tendons. Autologous AdSVF cells are used along with bone substitutes and other biological factors as an alternative to conventional bone grafting techniques owing to their promising osteogenic and vasculogenic capacity. It can also be used for treating osteonecrosis, meniscus tear, chondromalacia, and tendon injuries in veterinary practice. It has several advantages over in vitro expanded AdMSC, including precluding the need for culturing, reduced risk of cell contamination, and cost-effectiveness, making it ideal for clinical use.     

氟环唑对广东水稻纹枯病的田间防治试验

连续2009-2010两年田间药效试验结果表明,试验药剂氟环唑125g/L悬浮剂对水稻纹枯病具有较好防效,施药用量为有效成分75g/hm2处理的末次防效达72%以上,保产率达12%以上,是防治水稻纹枯病较为理想的药剂,对水稻安全。     

不同品质粗饲料日粮及添加酵母培养物对绵羊瘤胃内主要纤维素酶及纤维物质降解的影响

研究不同品质粗饲料混合日粮及添加酵母培养物对瘤胃内几种主要纤维素酶酶活及纤维物质降解的影响。试验将带有永久瘤胃瘘管的9只绵羊随机分成3组,以粗饲料分级指数GI为指标对粗饲料品质进行划分,在精粗比为2:8条件下配制3种混合日粮,形成含高GI粗饲料日粮组(H组,粗料GI值=2.89)、含中GI粗饲料日粮组(M组,粗料GI值=1.73)和含低GI粗饲料日粮组(L组,粗料GI值=0.65)。利用DNS法研究瘤胃内几种主要纤维素酶酶活的变化以及对纤维物质降解的影响。试验结果表明:3个试验组木聚糖酶的活性在饲后2h内下降,以后逐渐升高,到8h时达到最高并保持平稳;滤纸酶活和果胶酶活与粗饲料品质之间没有明显的规律性;添加酵母培养物(YC)后能够增加木聚糖酶活和果胶酶活,而M组和L组滤纸酶活有减少的趋势,但差异均不显著(P>0.05)。由本研究可知:3个试验组随着粗饲料品质的升高,木聚糖酶活有增加的趋势;果胶酶活和滤纸酶活未表现出一定的规律性。添加酵母培养物后有提高木聚糖酶活和果胶酶活的趋势,3个试验组的DM、NDF、ADF有效降解率都有不同程度的增加。     

Study on in vitro Culture of Donkey Skin Fibroblasts

This study was aimed to compare different culture methods for donkey skin fibroblasts to establish a highly efficient and rapid culture system. The dermal tissue of the healthy and 6-7 year old Xinjiang donkey was cultured for primary cells of skin fibroblast with tissue explants adherent method and double enzyme digestion method. Then,the growth characteristics,proliferation,cell cycle and mycoplasma contamination were detected and analyzed. The results showed that the fibroblast cells entered the logarithmic growth phase after cultured for 3 days by double enzyme digestion(0.25% trypsin digestion for 1 h and then 0.5% collagenase Ⅰ for 6 h), while it required 15 days with tissue explants adherent method. Flow cytometry analysis showed that nearly half of the cells were in G0/G1 stage,the other half in S+G2+M stage, while just 5.17% of the cells were apoptosis,which indicated that most of the cells were in the active stage of division and proliferation,and had strong vitality. Moreover, there was no mycoplasma contamination in the cultured cells. In summary,the culture system of donkey skin fibroblasts was rapidly and effectively established with the double enzyme digestion method in vitro.     

华山松种子园花粉管理的技术研究

从1986年起,我们对平坝华山松幼龄林种子园进行开花习性,人工辅助混合授粉和施肥促进开花的研究。研究结果表明,幼龄林种子园具有一定的结实能力,开雌花株数达45.5%。但是雌球花败育率高(73%),有雄花株少(12%),着果率低(59%)。通过人工辅助混合授粉,可提高着果率16.7%,施肥可增加雄球花花数1倍以上。     

The use of multilocation trials to select cultivars that are better than a control

Multilocation trials are a common means for evaluating new cultivars or strains. Their statistical analysis is often based on multiple comparisons with one or more controls. The purpose of this paper is to demonstrate the use of the Chen-Picket procedure for selecting cultivars that are better than a control, which does not require stringent statistical assumptions and allows conclusions about the probability that the selected subset contains all cultivars that are better than the control. This procedure appears preferable to the multiple t-test, which is often used in the evaluation of yield trials.     

Effects of processing and in vitro proteolytic digestion on soybean and yambean hemagglutinins

Some conventional processing methods were applied on yambean and soybean seeds and flour samples. They include soaking, fermentation, cooking whole seeds in the presence and absence of trona, autoclaving and dry heat treatment of flour samples. Hemagglutinating activity was assayed for after processing treatments. The hemagglutinating proteins from these seeds were classified based on their solubility properties. Effects of the presence of 0.01% concentration of trypsin, pepsin and proteases on agglutination of human red blood cells were also evaluated. Most processing methods, particularly cooking whole seeds for 1–2h, soaking and fermentation, reduced hemagglutinating activity on cow red blood cells. Size reduction accompanied by heat treatment was effective in eliminating hemagglutination. Both the albumin and globulin fractions of the soybean showed hemagglutinating activity but only the albumin fraction of the yambean had agglutinating properties. Proteolytic action of proteases was more effective in reduction of hemagglutinating activity than that of trypsin and pepsin.     

啤酒糟厌氧发酵生产沼气系统工程的设计

本文设计了一个以啤酒糟为原料的设备投资少、产气率高、综合利用好、经济效益高的沼气系统工程。归纳出该工程中主要设备的设计计算公式、结构类型、并绘制了图、可供农村能源工作者设计时参考。     

应用羊粪液取代瘤胃液两级离体消化试验的探索

应用羊粪液法和瘤胃液法两级离体消化试验的配对比较,通过七次不同日期所做的配对试验,测得同一种标准苜(?)草粉干物质消化率平均值分别为56.40±1.78％和56 64±3.53％,两音差异,(?)著、P>0.05)。测得32种饲草的干物质消化率平均值(?)为52.54±10.16％和50 12±10.74％,差异亦不显著(P>0.05)。瘤胃液法测草消化率(Y)和羊粪液测得相应结果(X)呈高度相关(r 0.97,P<0.01)。它(?)回归方程为:Y=1.022x-0.598 n=32,RSD=2.77。上述结果可为羊粪(?)的体外消化试验的完善和应用提供参考。