鼠源细胞TaqMan实时荧光定量PCR检测方法的建立

为对鼠源细胞进行鉴别检测,以鼠线粒体16S rRNA基因序列为靶位点设计特异性引物及探针,建立实时荧光定量PCR检测方法,并评价该方法的特异性及敏感性。结果显示,所建立的检测方法特异性好,针对鼠源细胞基因组荧光定量PCR扩增曲线良好,其他物种来源细胞基因组及生物制品原辅材料未出现特异性扩增曲线;敏感性高,基因拷贝数检出限度为45.3拷贝。本试验建立的荧光定量PCR检测方法能够有效地对鼠源细胞进行快速检测,为细胞质量控制提供了有效方法。     

土地利用动态监测中多源影像融合方法的比较研究--以陕北黄土丘陵沟壑区为例

在陕北黄土丘陵沟壑区,采用单一传感器的遥感影像提取土地利用信息,存在着识别的土地利用类别少、某些类别混分现象较严重、分类结果的精度较低等问题。以TM多光谱数据和SPOT全色光谱数据的融合为例,提出了适宜于该地区的两种影像融合方法:主成分变换法和乘积运算法,并从影像的光谱质量、纹理信息和目视效果等方面对其进行了对比与评价。结果显示,主成分变换法为较理想的融合方法。以陕北无定河流域为实验样区的土地利用自动分类结果表明,该方法的应用使土地利用各类别的提取精度都有不同程度的提高;水体、水田和城镇用地等面积较小的类别分类正确率提高达到10%以上;坡耕地与林草地的混分明显减少,分类精度均提高了5%以上;分类总精度从82.0%提高到89.2%,取得了良好的分类效果。此研究对于遥感影像融合技术的评价与应用进行了有益的探索,同时为该地区的土地利用动态监测提供了关键技术。     

棉花黄萎病菌毒素ELISA检测方法的建立及其应用

以纯化的棉花黄萎病菌毒素(PLPC)免疫新西兰白兔制备了PLPC特异性抗血清,建立了可特异性检测棉花黄萎病菌毒素的A蛋白双抗体夹心ELISA方法。应用建立的ELISA方法测定了病菌培养滤液、人工接种幼苗和大田病株不同组织中的毒素,结果表明:不同产毒能力菌株(VD 8和VD-5)在25℃下振荡培养3d后就能在培养滤液中检测到毒素,这比生物测定要早2～3d。将VD-8菌株的孢子以灌根方式接种泗棉3号幼苗5d后,就能从接种幼苗的茎杆和叶柄中检测到毒素,这比幼苗自然发病显症要早3～5d。在测定的30份大田病株茎杆、叶柄、叶脉样品中,阳性样品率为100%。这些结果表明建立的ELISA方法可用于菌株产毒能力的测定和大田棉花黄萎病的早期诊断。     

Model of Survivable Architecture

Critical information system in Interact is in face of increasingly security problems. More attention has been focused on the survivability of information system, which is the capability of system to continue its mission even in the presence of damage to the system. The authors first introduce the definition of survivability. According to four function properties of survivability, which are Resistance, Recognition, Recovery, and Adaption, the authors design a model of survivable system structure and then explain survivability requirement, survivability strategy in this model. Additionally, they provide possible technical solutions for the survivability of systems, categorized by different aspects of our survivability strategy.     

Wavelet Edge Detection Method Based-on Multi-scale Fuzzy Logic

A novel edge detection algorithm is proposed by implementing fuzzy image enhancement at different scales.The implement of this algorithm can reduce the negative influence bring from the non-maximum suppression algorithm which select only one threshold.The success of the approach depends on the selection of a general fuzzy operator(GFO) for the enhancement of contrast of region after smoothing of image at different scales.Consequently,it is more obvious that coarse intensity changes are obtained at a large scale whereas the fine details of intensity changes are obtained at a small scale.A pretty performance is obtained by used this work.     

MACHINE PARAMETER IDENTIFICATION AND FAULT DETECTION FOR THE ALTERNATOR SYSTEM

In this paper, the machine( physical) parameters of the alternator system are on-line estimated on the basis of the derived system continuous-tims model, using Recursive Least-squares Estimation and Inverse Calculation Method proposed by Authors. Therefore, the estimated machine parameters can effectively track the changes of their true values and accomplish the fault detection for the alternator system. This paper gives a new way for machne parameter estimation and fault detection of the alternator systems.     

五轴数控加工叶片无干涉刀位轨迹的计算

根据微分几何特性，提出五坐标数控加工叶片时对叶片的工作面和背面的刀位轨迹计算分开予以讨论，对工作面而言，利用平头立铣刀加工时不存在干涉，而对背面而言，可能存在干涉。根据干涉的种类，分别对全局干涉和局部刀具干涉提出了快速判定依据，并对干涉点进行了修正。指出利用Iso-scallop法可获得最优的轨迹和蔗，并给出了实例。