Antibacterial Cyclic Tripeptides from Antarctica-Sponge-Derived Fungus Aspergillus insulicola HDN151418

Three new aspochracin-type cyclic tripeptides, sclerotiotides M–O (1–3), together with three known analogues, sclerotiotide L (4), sclerotiotide F (5), and sclerotiotide B (6), were obtained from the ethyl acetate extract of the fungus Aspergillus insulicola HDN151418, which was isolated from an unidentified Antarctica sponge. Spectroscopic and chemical approaches were used to elucidate their structures. The absolute configuration of the side chain in compound 4 was elucidated for the first time. Compounds 1 and 2 showed broad antimicrobial activity against a panel of pathogenic strains, including Bacillus cereus, Proteus species, Mycobacterium phlei, Bacillus subtilis, Vibrio parahemolyticus, Edwardsiella tarda, MRCNS, and MRSA, with MIC values ranging from 1.56 to 25.0 µM.     

Role of cAMP and cGMP in the regulation of bFGF gene expression of rat cardiomyocytes

AIM: To investigate the role of cAMP and cGMP in the regulation of bFGF gene expression of rat cardiomyocytes. METHODS: 8-Bromo-cAMP and sodium nitroprusside were added into the media to raise the concentrations of cAMP and cGMP of cultured cardiomyocytes, in situ hybridization and immunohistochemistry methods were used to identify the mRNA and protein expression of bFGF. The amount of bFGF mRNA and protein expression were detected by computor imaging analysis system MIAS exe. RESULTS: At 24 h after adding 8-Bromo-cAMP(0 1 mmol/L), bFGF mRNA and protein expression elevated significantly , at 48 h and 72 h it still expressed higher than that of control group( P< 0.05). But after adding sodium nitroprusside (1 mmol/L),bFGF expression decreased significantly,and especially at 48 h. CONCLUSION: The regulation of bFGF expression of cardiomyocytes is related to cAMP signal pathway.     

Cyclic fluctuations of blue whiting (Micromesistius poutassou) linked to open‐sea convection processes in the northwestern Mediterranean

In the Mediterranean, blue whiting, Micromesistius poutassou, constitutes a traditional fisheries resource. Over several decades, blue whiting landings in the Catalan coast (northwestern Mediterranean) have displayed cyclical variations, of c. 6 yrs, slightly decreased to five in the last two decades, as shown through wavelet analysis. These fluctuations have persisted under very different levels of fishing effort. This study evaluates the hypothesis that deep‐water formation in the adjacent Gulf of Lions, and the enhanced primary productivity related to it, determines recruitment strength in blue whiting that results ultimately in the observed periodicity of the blue whiting landings. The link between landings and environmental drivers was explored using lagged cross‐correlations, with 0‐ and 1‐yr lag. The variables considered included large‐scale indices [North Atlantic Oscillation (NAO) and the Atlantic Multi‐decadal Oscillation (AMO)], Mediterranean climate indices [MO and Western Mediterranean Oscillation (WeMO)], and variables defining the local environmental conditions in the northwestern Mediterranean (sea‐air heat flux, winter air temperature anomaly and Rhône river runoff). Significant correlations were only found between landings (1961–2011) and sea‐air heat flux, which is generally taken as an indicator of processes of deep water convection, at 0 and 1‐yr lag. These results suggest that the observed fluctuations in blue whiting landings respond to oceanographic processes taking place in the Gulf of Lions.     

植物纤维复合墙体抗震性能研究

制作2榀内填材料分别为植物纤维生土基砌块和植物纤维水泥基砌块的1/2比例模型植物纤维复合墙体,进行低周反复加载抗震试验,介绍模型试件的主要破坏过程及受力特点;对比2榀墙体的破坏机制、承载力、滞回特性、抗倒塌能力等抗震性能。试验结果表明,内填植物纤维生土基砌块复合墙体发生"强柱弱板"的剪切型破坏,内填植物纤维水泥基砌块的复合墙体发生"强板弱柱"的弯曲型破坏;内填砌块的抗压强度、抗裂能力和弹性模量等参数的大小是影响植物纤维复合墙体受力性能的主要因素;两种植物纤维复合墙体都具有良好的抗震性能,应用于工程实际是完全可行的。     

氮素在枣树中贮藏位置、形态及循环利用研究

红枣作为人们日常生活中的常见水果之一,其有着极高的维生素,并具有滋阴补阳之 功效,这也使其深受人们的青睐与欢迎。现如今,枣树种植已经成为一种重要的农业产业,由于 枣树的生长在很大程度上取决于氮素,因此对枣树中氮素的贮藏位置、形态及其循环利用进行 研究是具有重要意义的,其能够为氮素的施肥提供科学的指导,从而促进枣树生长,提高红枣品 质。鉴于此,本文便对氮素在枣树中的贮藏位置分布、存在形态及循环利用做出深入的研究,以 期能够促进我国枣树种植产业的发展。     

类风湿因子与抗环瓜氨酸肽抗体在诊断类风湿关节炎中的应用

目的通过对类风湿关节炎（RA）患者及非RA患者血清中的类风湿因子（RF）与抗环瓜氨酸肽抗体（抗-CCP抗体）水平测定,探讨抗-CCP抗体在RA的诊断价值．方法用酶联免疫吸附方法对49例RA、6l例非RA患者测定血清抗CCP抗体及RF,并对检测结果进行比较．结果49例RA患者中抗-CCP抗体阳性23例,61例非RA患者抗...     

22L毒株朊蛋白错误折叠循环扩增方法的建立

参考Saborio和Soto创建的名为蛋白质错误折叠循环扩增(protein misfolding cyclic amplification,PMCA)的方法建立一种22L毒株系朊蛋白错误折叠循环扩增方法,以便研究22L毒株系异常朊蛋白(scrapie PrP,PrPSc)的合成机制。在阴离子去污剂十二烷基硫酸钠(SDS)存在的条件下,这种方法可以通过超声降解从而重复扩增产物,并在仓鼠脑匀浆中快速有效的模仿体内朊病毒PrPSc的复制,因此可以在几个小时内高浓度扩增PrPSc。已有试验结果发现,PrPSc体外转化率在使用粗提的脑组织匀浆时比使用纯化重组PrP更高,这个发现暗示着高效率的PrPSc转化可能还需要探索其他未知的宿主因素。研究结果表明,建立的这种改良的PMCA技术可用于研究PrPSc合成的生物化学机制,以此来进行诊断与治疗方法的研究。     

转变经济发展方式视角下县域循环经济的构建

转变经济发展方式体现在空间上,就是要转变县域经济的发展方式。本文在分析国外三大循环经济模式的基础上,结合我国地域和经济发展的特点,提出了我国转变经济发展方式应当大力发展区域中循环经济模式,即县域循环经济模式,并指出产业协同是发展县域循环经济的核心,最后探讨了政府在县域循环经济构建中的作用。     

“6R”原则在旅游循环经济发展中的应用

从人们对环境和发展问题的认识过程入手,提出发展旅游循环经济应遵循6个原则一再思考、减量化、再利用、再循环、再修复和再整合,即“6R”原则,并对其在旅游业中的应用作了具体阐述．     

普者黑旅游区生态型旅游产业集群发展战略

以云南丘北普者黑旅游区为例,提出该旅游区生态型旅游产业集群的构想,并寻找生态型旅游产业集群的发展思路,以加快其旅游产业的生态化和集群化发展进程．