Canine C-reactive protein (CRP) does not share common antigenicity with human CRP

Differences in antigenicity between human and canine C-reactive proteins were investigated by Western blotting analysis. It was confirmed that several commercial anti-human CRP sera reacted with canine CRP. However, 34 anti-canine CRP sera prepared by immunization of rabbits and goats with canine CRP all reacted with canine CRP but not with human CRP in either immunoelectrophoresis or Western blotting.Immunization with human CRP produced a cross-reacting antibody that reacted with canine CRP. Conversely, immunization with canine CRP did not produce a cross-reacting antibody that reacted with human CRP. These findings may be interpreted as showing that, while canine and human CRPs do not share common antigenicity, they do contain structurally similar antigenic determinants.Abbreviations CRP C-reactive protein - PBS phosphate-buffered saline - SDS-PAGE sodium dodecyl sulphate-polyacrylamide gel electrophoresis     

Crossed immunoelectrophoresis of fungal antigens in tissues as a means of diagnosing systemic aspergillosis and zygomycosis in cattle

A novel method for diagnosing bovine aspergillosis and zygomycosis is described. Rabbit hyperimmune antisera raised against somatic antigens ofAspergillus fumigatus andAbsidia corymbifera were used in crossed immunoelectrophoresis with supernatants from disintegrated tissues from acute necrohaemorrhagic mycotic lesions from cattle. The method specifically identified 4 of 5 lesions with aspergillosis and 2 of 5 lesions with zygomycosis. One lesion dually infected with aspergillosis and zygomycosis was negative. The method worked with unabsorbed sera, was specific, and required only standard electrophoretic equipment. It can therefore supplement chemical detection of fungi in tissues in the diagnosis of bovine aspergillosis and zygomycosis.Abbreviations APAAP alkaline phosphatase anti-alkaline phosphatase - XIE crossed immunoelectrophoresis     

水貂阿留申病诊断技术的研究进展

水貂阿留申病是由水貂阿留申病毒引起的自身免疫性疾病,目前尚无有效疫苗,每年结合打皮期筛查淘汰病貂是该病防控的主要手段.目前,水貂阿留申病的主要诊断方法是依据临床症状和血清的对流免疫电泳检测.对流免疫电泳需要在实验室内进行,使用范围局限.综述了国外该病诊断的最新研究进展,以期为该病诊断技术的开发提供思路.     

Serological reactivity of duck sera to three electrophoretically characterized extracts of Aspergillus

Three different antigens, whole culture, cell sap shaker culture and culture filtrate extracts of Aspergillus were characterized by crossed immunoelectrophoresis. Thirteen to fifteen precipitin lines were produced with their homologous antisera. Application of these extracts to the assay of wild duck sera showed that the whole culture extract was the most sensitive of the three antigens in detecting antibody. Countercurrent immunoelectrophoresis, used with a modified buffer, produced results comparable in sensitivity to immunodiffusion in detecting avian antibody.     

蝴蝶兰新品种‘翔凤’

‘翔凤’蝴蝶兰是以‘聚宝红玫瑰’蝴蝶兰为母本,‘红龙’蝴蝶兰为父本杂交育成的新品种,为深紫红中大花品种。平均主枝花朵数8.5朵,总花朵数9.0朵,花朵排列整齐有序;平均花径9.2 cm;温室栽培2月中旬始花,2-3月为盛花期。     

滇红食用玫瑰茎段增殖培养基的试验筛选研究

植物组织培养育苗是解决食用玫瑰种苗市场紧缺的一种重要技术途径,但其中最为关键的问题是如何高效高质量地实现茎段增殖。以滇红食用玫瑰为对象,通过实施L₉(3⁴)正交试验,研究不同因素对玫瑰茎段增殖的影响规律,并分析筛选适宜茎段增殖的最佳培养基。结果表明：不同的试验处理,滇红食用玫瑰的茎段分化增殖差异较大,表现出了4 种主要的植物形态类型;基础培养基对茎段的增殖系数影响显著(P＜0.05),而6-BA、NAA 和GA₃ 3 个因素对茎段增殖系数的影响没有达到显著水平(P＞0.05),4 种因素对增殖系数影响的主次关系为：基础培养基＞6-BA＞NAA＞GA₃;最佳茎段增殖培养基为WPM+6-BA 1.0 mg/L+NAA 0.01 mg/L+GA₃ 0.5 mg/L+白砂糖30 g/L,其具备的优点为茎段成株率高、植株高、笔直整齐、茎杆粗壮、节间较长、枝条多、节段数多、增殖系数大。研究结果可应用于滇红食用玫瑰的工厂化组织培养育苗。     

蝴蝶兰黄花系品种Phalaenopsis‘Taipei Gold’和P.‘Sara Gold’杂交及其后代的遗传表现

以蝴蝶兰Phalaenopsis黄花系品种P.‘Taipei Gold’(2n=3x=57)作母本进行杂交育种有一个突出的问题是不育性障碍,为了克服这种障碍,多年来选择不同的父本进行杂交,终于发现采用P.‘Sara Gold’作父本进行杂交,收到蒴果1个,培育出杂交后代群体180株,其中有开花株99株。对杂交后代株高、冠幅、着花量、花径、花箭数、侧枝数及花色、唇瓣颜色和花瓣上的斑点进行观测记载。结果表明:杂交后代生长发育指标优于双亲的株数百分比:株高、冠幅、着花量、花径、侧枝数和花箭数分别占67.68%,86.87%,61.62%,24.24%,21.21%和26.26%;花瓣和唇瓣的颜色,基本遗传了双亲的特点,91%的后代花瓣颜色与双亲相似,74%的后代唇瓣颜色与双亲相似,90%的后代花瓣上出现了斑点。以P.‘Taipei Gold’作为母本成功杂交在国内首次报道,为蝴蝶兰黄花系杂交育种提供了实践依据。     

板栗杂交后代3个重要经济性状的遗传特点

通过对4个杂交组合417株实生苗的分析,研究了3个经济性状的遗传特点.结果表明:坚果单粒重、刺苞大小和出实率的遗传均符合数量性状连续变异的遗传特点.单粒重和刺苞大小表现同样的遗传趋势,遗传中非加性效应占有很大比重,杂交后代由于非加性效应解体,单粒重量和刺苞大小有变小的倾向.出实率的遗传以加性效应控制为主,杂交后代由于非加性效应累加,出实率有提高的倾向.     

马·花杂交F1的鲜茸产量和繁殖方法及探讨

系统报道了48年来我国几个主要单位开展家养马鹿(♀)与梅花鹿(♂)杂交,即马.花杂交试验研究工作及其马.花杂交F154只290副茸的鲜茸产量和2只创记录者各锯龄的锯茸规格茸型和鲜茸产量、本交繁殖方法,并探讨了采用马鹿人工授精和同期发情等现代繁殖技术,大规模高效开展育成杂交,尤其清.双杂交和清.西杂交,乃至培育茸肉血兼用型鹿与该项最佳杂交组合的显效性,为养鹿业种鹿场、良繁体系、良繁基地和大型企业名牌鹿的产业化高效生产经营提供了新理念和科学依据。     

贵州省玉米三交种的选育与利用

简要介绍了玉米三交种的特点,对玉米三交种的生产特性与单交种进行了比较,同时通过对贵州省玉米三交种的选育与利用情况分析,认为三交种更适于山区复杂生产条件和低投入、粗放管理的生产模式,应加强三交种选育的理论研究和投入.总结了贵州省玉米三交种的亲本来源和组配方式三交种的父本主要采用植株高大、雄穗发达、花粉量大、配合力高的自交系,来源为热带种质和地方种质.组成母本杂交种的自交系的组配方式一般采用地方种质×含有地方种质的自交系、地方种质或含有地方种质的自交系×温带种质.