模猪场猪瘟野毒感染情况调查

利用ELISA方法对广西6个规模猪场送检的1626份猪血清进行猪瘟野毒感染情况调查。结果6个规模猪场均存在猪瘟野毒感染,其中母猪感染率为7.86%~29.21%,平均为17.52%,种公猪感染率为0%~23.52%,平均为11.83%,育肥猪感染率为5%~22.45%,平均为15.5%,断奶仔猪感染率为8.24%~18.57%,平均为12.69%。此检测结果与猪场临床发病情况基本一致,病猪多表现为繁殖障碍型、温和型的非典型猪瘟。     

改进粒子群算法在车间作业调度中的研究

车间作业调度（JSP）是典型的NP难题,传统求解方法都有各自的特色和不足。免疫系统强大的信息处理能力为人工免疫应用提供了丰富的暗示,因此,免疫算法被提出,并逐渐应用于许多工程实际。针对车间作业调度这个优化问题的难处理性,提出了基于免疫粒子群算法（IPA）的JSP求解方法。在该求解方法中,结合免疫原理和粒子群算法应用于JSP的算法流程;算法采用基于操作的编码方式;依据接种疫苗和变异、免疫选择的机制来设计算子。并通过仿真,证明了IPA算法在JSP中的有效性。     

弓形虫棒状蛋白2研究进展

棒状蛋白2(ROP2)为弓形虫生活史各期均能分泌的抗原,具有高度的保守性和免疫反应性.综述了近年来关于ROP2分子生物学特征、免疫特性、疫苗的研究进展.     

猪源马链球菌兽疫亚种灭活疫苗对小鼠的免疫效力评价

 【目的】评价中国不同地区猪源马链球菌兽疫亚种分离株抗原性的差异,为猪链球菌病疫苗的研制提供指导。【方法】取从国内10省市分离的10株猪源马链球菌兽疫亚种分离株,分别制备灭活疫苗,免疫12周龄ICR小鼠,用同源菌株和ATCC35246株攻击。【结果】以同源菌株攻击免疫小鼠,除CG-74-63株外,其余菌株的保护率均在90％以上;而以异源菌株ATCC35246进行攻击,免疫小鼠的免疫保护率为80%～100%。【结论】中国不同地区的猪源马链球菌兽疫亚种分离株的抗原性差异不大,单一菌株制备的疫苗具有保护性。     

Effect of cell mediated immunity regulation of duck enhanced by duck IFN-α eukaryon expression plasmid and inoculated with DPV attenuated vaccine by gene-gun

In order to study the effect of cell mediated immunity regulation of duck IFN-α eukaryon expression plasmid (pcDNA-SDIFN-α) on duck plague virus (DPV) attenuated vaccine in ducks, pcDNA-SDIFN-α was administered to 28-day-old ducks at doses of 1, 3 and 6 μg per duck, respectively, by gene-gun. PBS and empty vector pcDNA were used as control. Fifteen days later, all ducks were injected with DPV attenuated vaccine and blood samples were collected at 3, 7, 14, 21, 28, 35, 49, 63 and 84 days after injection. T-lymphocyte proliferation tests (MTT) were used to detect the T-lymphocyte proliferation in the peripheral blood (PBL) of ducks. Blood samples collected at 7, 14, 21, 28, 35 and 49 days after injection were detected by fluorescence-activated cell sorter (FACS) for recording the number of CD₃ ⁺ T-lymphocytes of ducks. Results were as follows: (1) Reaction of T-lymphocytes in PBL to ConA (OD value) of ducks treated with pcDNA-SDIFN-α was higher than that of PBS and pcDNA control groups in 3–84 days. There were highly significant differences between the 1 μg per duck group and the two control groups in 3–84 days (P ⩽ (0.01), between the 3 μg per duck group and the two control groups in 3–84 days (P ⩽ 0.01, P ⩽ 0.05), and between the 6 μg per duck group and the two control groups in 7–49 days (P ⩽ 0.01, P ⩽ 0.05). The significant difference was also present between the groups of 1, 3 and 6 μg per duck in 3–35 days (P ⩽ 0.05). However, there was no significant difference between the 3 and 6 μg per duck groups (P ⩾ 0.05). The pcDNA control group was higher than PBS control group, but no difference was detected (P ⩾ 0.05). (2) Change of the number of CD₃ ⁺ T-lymphocytes in ducks administered with different doses of pcDNA-SDIFN-α was higher than that of PBS and pcDNA control groups in 7–49 days. The change in the 1 μg per duck group was significantly higher than that in PBS and pcDNA control groups in 14–49 days (P ⩽ 0.01). There were significant differences between the 3 μg per duck group and the two control groups in 21–49 days (P ⩽ 0.01, P ⩽ 0.05) and between the 6 μg per duck group and the two control groups in 7–49 days (P ⩽ 0.01, P ⩽ 0.05). However, no significant differences among the groups of 1, 3, and 6 μg per duck groups (P ⩾ 0.05) and between the two control groups (P ⩾ 0.05) were found. The results indicated that pcDNA-SDIFN-α administered 15 days before injection of DPV-attenuated vaccine could significantly enhance cellular immunity induced by DPV-attenuated vaccine. pcDNA-SDIFN-α is an excellent DPV-attenuated vaccine molecular adjuvant and the best result can be obtained with the dose of 1 μg per duck of pcDNA-SDIFN-α inoculated by gene-gun. __________ Translated from Acta Veterinaria et Zootechnica Sinica, 2007, 38 (10): 1066–1071 [译自: 畜牧兽医学报]     

GC02鸡球虫病疫苗免疫对鸡红白细胞数的影响

通过GC02鸡球虫病疫苗免疫雏鸡,研究其对鸡红白细胞数的影响。选择100只1日龄肉杂鸡随机分成两组,每组50只。Ⅰ组为对照组,Ⅱ组为试验组,饲喂同种日粮。Ⅱ组分别在7日龄、14日龄给每只鸡免疫8000个GC02鸡球虫病疫苗孢子化卵囊。Ⅰ组和Ⅱ组分别在21、28、35和42日龄采血,进行红、白细胞计数。结果表明在21日龄,Ⅱ组红、白细胞数与Ⅰ组相比差异均显著（P〈0．05）,28、35和42日龄Ⅱ组与Ⅰ组相比差异均不显著（P〉0．05）。从试验组与对照组的红、白细胞数百分比[（E／C）％]来看,免疫后试验组红细胞数先减少,21日龄最低（为对照组的93．89％）,随后慢慢升高,超过对照组,最大值出现在35日龄（为对照组的100．36％）,42日龄又下降;试验组白细胞数均高于对照组,21日龄最高（为对照组的106．64％）,随后慢慢降低,最低值出现在35日龄（为对照组的100．85％）,42日龄稍稍上升。说明GC02鸡球虫病疫苗免疫雏鸡后,对鸡的红白细胞数影响不大。     

乌桕根皮醇提物萃取部位抗猪大肠杆菌活性分析

[目的]探讨乌桕根皮醇提物各部位对猪大肠杆菌4种血清型的抗菌活性。[方法]用95%乙醇80℃回流提取乌桕根皮,将醇提液浓缩后依次用石油醚、乙酸乙酯和正丁醇萃取,最后得到萃余的水相,测试各部位的抗菌活性。[结果]醇提物的石油醚部位、正丁醇部位和水部位没有抑制作用,乙酸乙酯部位对血清型K88F、41和987P具有抑制效果,而对血清型K99无效。因此,用中药材防治猪大肠杆菌疾病时,应该考虑到血清型差异。[结论]乌桕根皮提取物可以在一定程度上防治大肠杆菌引起的猪病。     

从全长cDNA克隆恢复猪瘟病毒方法的研究

以携带猪瘟病毒Thiverval株全长cDNA克隆的pAC/F101/T1-7载体质粒为模板,在体外转录病毒基因组RNA,并转染PK-15和BHK-21细胞,通过传代、RT-PCR、免疫过氧化物酶细胞单层试验鉴定,成功地在两种细胞中拯救出具有感染性的病毒粒子。同时,通过2种细胞转染效率的对比试验,成功建立了利用高转染效率的其它真核细胞作为病毒拯救的过渡细胞,然后再在猪肾细胞系上进行增殖的病毒拯救方式,极大提高了猪瘟病毒拯救的效率。     

猪瘟病毒囊膜蛋白E2基因真核分泌型表达载体的构建及其表达

【目的】构建猪瘟病毒囊膜蛋白(E2 protein)基因的真核表达载体,并将其在猪脐静脉血管内皮细胞中进行表达。【方法】采用PCR技术扩增出E2蛋白全长基因(E2qc)序列和去除跨膜基因(E2sh)序列,并将其克隆入真核表达载体pSecTag2(A)中,构建pSecTag-E2qc和pSecTag-E2sh表达载体,分别进行PCR、双酶切及测序鉴定。用脂质体法将阳性克隆瞬时转染猪脐静脉血管内皮细胞,对转染细胞进行RT-PCR检测目的基因的转录情况,同时对转染细胞及细胞上清液进行SDS-PAGE和Western-blot分析,以检测目的蛋白的表达情况。【结果】成功克隆了E2蛋白基因全长序列1119bp和去除E2蛋白跨膜基因序列999bp的目的基因。构建的表达载体经PCR、双酶切法及测序鉴定均无误。转染后细胞的RT-PCR结果显示,目的基因被成功转录。转染后细胞及细胞上清液的SDS-PAGE和Western-blot结果显示,目的基因被成功表达。【结论】成功构建了猪瘟病毒E2蛋白的真核表达载体,转染猪脐静脉血管内皮细胞后,分泌表达了猪瘟病毒E2重组蛋白。     

鸡减蛋综合症油乳剂灭活疫苗的免疫效果观察

将试验鸡分成3组,第一组、第二组和第三组鸡分别接种鸡减蛋综合症油乳剂灭活苗1ml、 1．5ml和2ml。接种疫苗后3、4、5、6、7、9、11、13、15d采取各组鸡血,检测HI抗体。接种2ml油乳剂灭 活苗的组鸡HI抗体效价上升快,效价高,但下降也非常快,较适合于紧急接种。1ml和1．5ml油乳剂 灭活苗接种鸡HI抗体效价上升较慢,产生免疫力的时间较迟,但维持高抗体水平的时间较长,适于 预防接种。