Oncology Virtual Issue Articles

The following three articles have been published online as part of the EVE Oncology Virtual Issue which is available online at wileyonlinelibrary.com/journal/eve     

薄木层积材颈椎夹板构造机制及其弹性模量计算

采用现代颈椎夹板设计理论,从微观结构研究薄木层积材特性,构造新型薄木层积材颈椎夹板.文中给出模压颈椎夹板的薄木最优尺寸,求出其理论弹性模量,给出对强度产生影响的相关系数,并推导出这些系数和薄木层积材颈椎夹板的弹性模量之间定量的数学关系和力学表达式,为颈椎夹板复合弹性力学的微观力学理论提供基础,并给出试验证明.     

人体颈部动力学响应分析有限元模型的建立和验证

根据汽车碰撞事故中人体颈部损伤防护技术研究的需要。建立了一个人体颈部三维有限元模型。该模型参照人体解剖结构模拟了颈椎骨、椎间盘、小关节、韧带、肌肉和软骨等组织，共由15811个单元构成。各组织的材料特性采用了弹性、粘弹性、和刚性材料模型来描述．模型用前碰撞志愿者实验数据进行了验证。其头部的加速度、位移曲线与实验数据得到了较好的吻合。本文初步研究了软组织特性参数对于颈部运动的影响。该模型具有较高的生物逼真度，可对头颈部在汽车碰撞载荷条件下的加速度、速度、位移等动力学响应参数进行分析研究。     

刚棘颚口线虫的扫描电镜观察

刚棘颚口线虫扫描电镜显示：头球顶端有两片半球形的大侧唇，大侧唇背面有1对双乳突和1个头感器。在轮节皮棘第6-7排或第8-9排或第10-11之间各有1对颈乳突，与樊氏等及古贺正崇等描述颈乳突位置有所差异。并观察到雄虫尾端长交合刺的皮纹上有细小的凹陷小乳，其顶端有开口处；有2对尾乳突。雌虫阴门位于虫体腹面中线之后；尾端前腹面有半月状的肛门，有1对尾乳突。其中头钩、体棘、交合刺和尾乳突等形态特征与前人记述相似。     

猪颈部食管传出神经元的起源——用HRP法研究

将 HRP 注入颈部食管壁后,在延髓的两侧面神经后核出现大量密集的中、小型标记细胞,在疑核的全长可见散在的大型细胞,在迷走神经背核可见少量小型细胞,以核的前区腹外侧缘标记细胞较多。在闩以上的孤束内侧核和第一颈髓的中间外侧核(疑后核)也看到零星标记细胞。颈前神经节和星状神经节出现大量标记细胞,而以颈前节标记细胞较多,还多次发现小标记细胞紧偎着大标记细胞配对的现象。     

Anti-Sonic hedgehog blocking antibody enhances killing effect of PBMCs on cervical carcinoma HeLa cells

AIM: To investigate the effect of anti-Sonic hedgehog(Shh) blocking antibody on the killing effect of peripheral blood mononuclear cells(PBMCs) on cervical carcinoma HeLa cells. METHODS: The expression levels of Shh and Shh signaling molecules in HeLa cells were detected by immunocytochemistry and RT-PCR. PBMCs from health peoples were isolated by the method of Ficoll density gradient centrifugation, and then co-cultured with HeLa cells in vitro. The expression of CD3, CD69 and CD71 was assayed by flew cytometry. The concentrations of IFN-γ, IL-10 and IL-4 in culture supernatants were detected by ELISA. The killing effect of PBMCs on HeLa cells was observed under microscope. RESULTS: Shh and Shh signaling molecules were expressed in HeLa cells. The level of Shh expression didn't change significantly in the 6th passage of HeLa cells. CD3⁺ cells were increased in the co-culture system. The expression of CD69 and CD 71, and the secretion of IFN-γ were increased, while the secretion of IL-10 was decreased in the co-culture system treated with anti-Shh blocking antibody. Anti-Shh blocking antibody has no effect on the secretion of IL-4. The killing effect of PBMCs on HeLa cells was strengthened by anti-Shh blocking antibody. CONCLUSION: Anti-Shh blocking antibody promotes the activation of PBMCs and enhances the killing effect of PBMCs on cervical carcinoma HeLa cells.     

奶牛宫颈粘液CMPx,CMEC与发情及排卵的关系

选取３０头健康黑白花奶牛，在发情期不同时间测定宫颈粘液过氧化物酶（ＣＭＰｘ）的活性及电导率（ＣＭＥＣ），以探讨ＣＭＰｘ、ＣＭＥＣ与发情及排卵的关系。结果表明，ＣＭＰｘ在卵泡成熟期明显下降，与卵泡初期及排卵初期的酶活性差异极显著（Ｐ＜０．０１）；而ＣＭＥＣ在卵泡初期、卵泡成熟期及排卵初期变化不显著（Ｐ＞０．０５）。本研究提示，ＣＭＰｘ在奶牛发情期有特定的变化规律，可以作为预告排卵的指标     

人类表皮生长因子受体2蛋白在宫颈癌组织中的表达及意义

目的观察宫颈癌组织中人类表皮生长因子受体2（HER-2/neu）蛋白表达及其在宫颈癌发生发展中的作用。方法应用免疫组织化学方法检测20例正常宫颈组织、30例宫颈上皮内瘤样病变（CINⅠ-Ⅲ）和50例宫颈癌组织中HER-2/neu蛋白表达。结果宫颈癌组织中HER-2/neu蛋白表达显著高于宫颈上皮内瘤样病变（P〈0.01）,正常宫颈组织未见阳性表达;宫颈癌组织中HER-2/neu蛋白表达与肿瘤浸润深度、淋巴结转移、临床分期密切相关（P〈0.05）。结论 HER-2/neu蛋白表达上调可能在宫颈癌发生发展中起重要作用。