Potpourri oil toxicity in cats: 6 cases (2000–2007)

Objective – To describe the clinical and laboratory features, treatment, and outcome of cats with a history of potpourri oil exposure. Design – Retrospective study. Setting – Veterinary teaching hospital. Animals – Six client‐owned cats. Measurements and Main Results – The medical records of animals with oral or lingual ulceration and exposure to potpourri oil from January 2000 to December 2007 were reviewed. Information obtained from the medical records included signalment, product to which the animal was exposed, clinical signs, physical examination findings, diagnostic test results, treatment, duration of hospitalization, and outcome. Six cats qualified for inclusion in the study; 3 were castrated males and 3 were spayed females. Age range was 0.3–3.6 years. All cats had severe oral and lingual ulceration. Additional clinical findings included hyperthermia, tachypnea, ptyalism, and lethargy. Treatment of these cats included IV fluids, analgesic therapy, antimicrobial therapy, and gastrointestinal protectants. Two of these cats received an esophagostomy tube for nutritional support. Duration of hospitalization was 0–5 days. All cats survived to discharge. Follow‐up for 3 cats revealed complete healing of the oral ulceration. Conclusions – Potpourri oil toxicity should be considered as a differential diagnosis in any cat with oral and lingual ulceration. Prognosis for recovery is good with supportive care.     

禽流感HA基因疫苗脂质体的制备及其理化性质

用改良的逆相蒸发法制备了Ｈ７亚型禽流感保护性抗原血凝素（ＨＡ）基因重组质粒ｐＳＶＨ７脂质体。电镜观察表明,该脂质体多呈球形的单层或多层结构,脂质体粒径分布均匀,粒径范围２０～７００ｎｍ,平均粒径１６３ｎｍ,主要粒径分布位于５０～２００ｎｍ（８４．８０％）。重组质粒ＤＮＡ在脂质体的制备过程中保持了稳定性,并能抵抗ＤＮＡ酶的水解破坏作用。吸收光谱表明,在２４８ｎｍ及２６８ｎｍ处脂质体有２个特征吸收峰,ＤＮＡ的存在不改变吸收峰的位置,但能增加吸收峰的强度。     

阳离子改性茧衣的吸附性研究

为了拓展茧衣在过滤和废水处理领域的应用,对茧衣进行阳离子接枝改性,测试了改性茧衣对活性染料和细菌的吸附性及稳定性。通过染液Abs值和染液颜色的变化以及平板茵落数来判断改性茧衣对染料和细菌的吸附作用。结果表明：改性茧衣对活性染料和细菌的吸附效果明显,有较强抑茵效果;经高温高压处理后的改性茧衣对染料和细菌的吸附作用没有大的影响。该研究结果为茧衣在吸附材料、过滤材料、废水处理等方面的应用提供了有益的借鉴和参考。     

猪肠道碱性氨基酸转运载体(CAT1)mRNA表达的组织特异性和发育性变化

本研究旨在探讨不同品种猪肠道碱性氨基酸转运载体（CAT1）mRNA表达的组织特异性和发育规律。试验选取1、7、26、30、60、90和150日龄长白和蓝塘公猪各5头（同一品种同一日龄且体重接近）,共70头,测体重后屠宰,采集十二指肠、空肠、回肠和结肠组织样品。以18S基因为内标,用实时荧光定量RT-PCR法（SYBR Green Ⅰ试剂盒）检测CAT mRNA在60日龄长白猪十二指肠、空肠、回肠和结肠表达的组织特异性,以及在长白和蓝塘猪不同日龄其十二指肠、空肠、回肠表达的发育性变化。结果显示：60日龄长白猪CAT1 mRNA的表达丰度从十二指肠到回肠呈逐渐升高的趋势,到结肠开始下降,回肠极显著高于其他3个肠段（P〈0．01）,十二指肠最低;十二指肠和空肠CAT mRNA的表达在1～26d（即哺乳期）都呈上升的趋势,随后都开始有所下降;蓝塘猪十二指肠CAT1 mRNA的表达丰度在150d时显著低于其他各个阶段（P〈0．05）,而长白猪90和150d都显著低于其他各阶段（P〈0．05）;空肠CAT1 mRNA的表达在26～150d各阶段都差异不显著,而26d显著高于1和7d（P〈0．05）;1～60d长白和蓝塘猪回肠CAT1 mRNA的表达都呈逐渐上升的趋势,60d后都显著下降（P〈0．05）。两品种猪不同日龄时十二指肠和空肠CAT1 mRNA的表达量都没有显著差异（P〉0．05）;长白猪回肠CAT mRNA表达在26d时显著高于蓝塘猪（P〈0．05）;在90和150d时,长白猪都显著低于蓝塘猪（P〈0．05）,其他各阶段没有显著差异。结果说明,CAT mRNA在不同肠段及不同发育阶段的表达存在明显的差异,这可能与肠腔中氨基酸的浓度和氨基酸的需要水平及相关激素水平有关。     

阿米卡星脂质体的研制及体外抑菌试验

为研究阿米卡星脂质体的体外抑菌效果 ,采用逆相蒸发法制备阿米卡星脂质体 ,利用Hantzsh反应测定其包封率 ,并比较阿米卡星脂质体和游离阿米卡星对大肠杆菌、链球菌、巴氏杆菌、金葡菌的最小抑菌浓度和最小杀菌浓度。结果表明 :阿米卡星脂质体的包封率较高 ,达到6 1 .8%,阿米卡星脂质体对大肠杆菌、链球菌、巴氏杆菌、金葡菌的最低抑菌浓度分别约为游离阿米卡星的 1/3.5,1/7,1 /2 ,1/2 ,最低杀菌浓度分别约为游离阿米卡星的 1/4,1 /4,1/1 .8,1 /4,证明阿米卡星脂质体的体外抗菌作用明显优于游离阿米卡星     

脂质体法转染水牛肾脏成纤维细胞条件的优化

试验旨在研究脂质体介导基因转染水牛肾脏成纤维细胞时如何获得较高的转染效率。本研究将以绿色荧光蛋白基因为标记基因,利用Lipofectamine LTX介导外源DNA转染水牛肾脏成纤维细胞,探讨脂质体用量、质粒用量、质粒大小、转染时间、细胞初始接种密度对转染效率的影响。结果表明,4 μg pmaxGFP质粒DNA与4 μL脂质体转染6 h,其细胞活性达98%,可获得较满意的转染效率。     

动物免疫增强剂的研究进展

免疫增强剂可促进动物对抗原的免疫应答反应,显著增强疫苗的免疫效果。文章综述了动物疫苗研究领域中的免疫增强剂,如免疫刺激复合物、脂质体、细胞因子、胞嘧啶-鸟嘌呤寡脱氧核苷酸和天然产物对动物疫苗免疫效果的影响,并对免疫增强剂的发展趋势作了展望。     

广西红壤去除水中阳离子染料的研究

以广西红壤为吸附剂,研究了其对阳离子染料的吸附性能。结果表明,广西红壤对所研究的5种阳离子染料均有较强的吸附能力,吸附速度快,吸附量不随pH的变化而变化,吸附规律符合Langmuir等温吸附方程和Freundlich等温吸附方程。     

斜叶黄檀精油脂质体的制备及其抑制黑色素合成的研究

研究不同浓度斜叶黄檀精油(0、5、10、15、20 mg/L)对制备的脂质体稳定性及其抑制斑马鱼胚胎黑色素合成的影响.通过粒径、多分散系数、Zeta电位、包封率指标来表征斜叶黄檀精油脂质体,评价其在4℃下储藏4周的稳定性.结果发现,10 mg/L的斜叶黄檀精油脂质体在储藏过程中最为稳定,包封率达到最大,为45.10％±...     

Encapsulated Spirulina Powder Feed for the Nutritional Enrichment of Adult Brine Shrimp (Artemia salina)

Abstract

Liposomes were prepared in the laboratory with lipids obtained from chicken egg yolk to encapsulate Spirulina powder. Artemia napulii were hatched and fed with lipid-encapsulated Spirulina or Spirulina powder. After 20 days of rearing, the experimental A. salina were harvested and measured for total body length and total content of protein and carbohydrates. Total body length was 45% higher, protein content was 49% higher, and carbohydrate was 35% higher in Artemia fed with liposomes as compared with those fed only Spirulina powder.